HERC5 Antibodies

AND HERC5 ELISA kits, HERC5 Proteins

Many biological assays use antibodies to detect HERC5, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with HERC5 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop HERC5 antibodies...

We validate the specificity of these antibodies to HERC5 by testing them on tissues known to express HERC5 positively and negatively. Browse below to find the HERC5 antibody that suites your experiment. We have 3 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

Previous: SEPTIN9 Next: CKLF

HERC5 Infographic by Boster Bio

All HERC5 Products

Filter by Application:

Product

Figures

Specificity

Applications

Price

Anti-HERC5 Antibody Picoband®

5 Q&As

Western blot analysis of HERC5 using anti-HERC5 antibody (A05285-2)

IF analysis of HERC5 using anti-HERC5 antibody (A05285-2)

Human

ELISA, IF, ICC, WB

$370
Cat # A05285-2
100 μg/vial

Human HERC5 Recombinant Protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

SDS-PAGE, Denatured

$250
Cat # PROTQ9UII4
Starting from 20ug

HERC5 (NM_016323) Human Over-expression Lysate

Human

$960
Cat # LS051191
20 µg, 100 µg

HERC5 Overview

Facts about E3 ISG15--protein ligase HERC5.

HERC5 3D structure. Source: alphafold

E3 ISG15--protein ligase HERC5 (HERC5)

Acts as a positive regulator of innate antiviral response in cells induced by interferon. Functions as part of the ISGylation machines that recognizes target proteins in a broad and comparatively non-specific manner.

Catalyzes ISGylation of IRF3 which leads to sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 that attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets.

Gene Information

Human
Gene Name:	HERC5
Uniprot:	Q9UII4
Entrez:	51191

Family

Belongs to:

No superfamily

Alternative Names

CEB1E3 ISG15--protein ligase HERC5; CEBP1; cyclin-E binding protein 1; Cyclin-E-binding protein 1; EC 6.3.2; EC 6.3.2.-; HECT domain and RCC1-like domain-containing protein 5; hect domain and RLD 5; HECT E3 ubiquitin ligase; probable E3 ubiquitin-protein ligase HERC5

Molecular Weight

Mass (kDA):
116.852 kDA

Genomic Context


Human

Location:	4q22.1
Sequence:	4; NC_000004.12 (88456604..88506170)

Tissue Specificity

Expressed in testis and to a lesser degree in brain, ovary and placenta. Found in most tissues at low levels.

Subcellular Localizations

Cytoplasm, perinuclear region. Associated with the polyribosomes, probably via the 60S subunit.

Diseases

Influenza
Innate Immune Response
Hiv Infections
Malignant Neoplasms
Neoplasms
Bacterial Infections
Germline Mutation Abnormality
Autoimmune Diseases
Carcinogenesis
Infective Disorder
Virus Diseases
Autoimmune Reaction

Leukemia
Sclerosis
Neoplasm Metastasis
Malignant Neoplasm Of Breast
Hepatitis C, Chronic
Dermatomyositis
Premature Aging Syndrome

Pathways

Conjugation
Cell Cycle
Pathogenesis
Osteoclast Differentiation
Cell Proliferation
Cellular Senescence
Immune Response
Dna Repair
Neuron Differentiation
Virulence
Localization
Cell Cycle Arrest

Senescence
S Phase
Mitotic Recombination
Chromosome Condensation
Viral Replication
Nuclear Import

PTMs

Isgylation

Ubiquitination

Research Areas

Cell Cycle and Replication

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/HERC5

References

PMID: 10581175 by Mitsui K., et al. A novel human gene encoding HECT domain and RCC1-like repeats interacts with cyclins and is potentially regulated by the tumor suppressor proteins.

PMID: 15331633 by Kroismayr R., et al. HERC5, a HECT E3 ubiquitin ligase tightly regulated in LPS activated endothelial cells.