Click image to see more details
-
-
-
-
-
+2
Product Info Summary
| SKU: | P03300 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho-DAPK3 (T265) Antibody
SKU/Catalog Number
P03300
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Phospho-DAPK3 (T265) Antibody catalog # P03300. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-DAPK3 (T265) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03300)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human DAPK3 around the phosphorylation site of Thr265. AA range:241-290
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P03300 is reactive to DAPK3 in Human, Mouse, Rat
Observed Molecular Weight
52 kDa
Calculated molecular weight
52.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P03300 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DAPK3 (Phospho-Thr265) Antibody
Click image to see more details
Western Blot analysis of HuvEc cells using Phospho-DAPK3 (T265) Polyclonal Antibody
Click image to see more details
Western blot analysis of lysates from HUVEC cells, using DAPK3 (Phospho-Thr265) Antibody. The lane on the right is blocked with the phospho peptide.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
Click image to see more details
Immunofluorescence analysis of Hela cell. 1,DAPK3 (phospho Thr265) Polyclonal Antibody (red) was diluted at 1:200 (4° overnight). α-tubulin Monoclonal Antibody (8F11) (green) was diluted at 1:200 (4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
Click image to see more details
Immunofluorescence analysis of A549 cells, using DAPK3 (Phospho-Thr265) Antibody. The picture on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho-DAPK3 (T265) Antibody (P03300)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho-DAPK3 (T265) Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Phospho-DAPK3 (T265) Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
3 Customer Q&As for Anti-Phospho-DAPK3 (T265) Antibody
Question
We are currently using anti-Phospho-DAPK3 (T265) antibody P03300 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
The anti-Phospho-DAPK3 (T265) antibody (P03300) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-26
Question
I am interested in to test anti-Phospho-DAPK3 (T265) antibody P03300 on mouse brain for research purposes, then I may be interested in using anti-Phospho-DAPK3 (T265) antibody P03300 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
The products we sell, including anti-Phospho-DAPK3 (T265) antibody P03300, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-19
Question
Is this P03300 anti-Phospho-DAPK3 (T265) antibody reactive to the isotypes of DAPK3?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
The immunogen of P03300 anti-Phospho-DAPK3 (T265) antibody is Synthesized peptide derived from human DAPK3 around the phosphorylation site of T265. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-04
