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Product Info Summary
| SKU: | M01389-3 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-Hexokinase II (HK2) Mouse Monoclonal Antibody [Clone ID: OTI4C5]
SKU/Catalog Number
M01389-3
Size
100 µl
Description
Boster Bio Anti-HK2 (Hexokinase II) mouse monoclonal antibody, clone OTI4C5 (formerly 4C5). Catalog# M01389-3. Tested in FC, IF, WB. This antibody reacts with Human, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Hexokinase II (HK2) Mouse Monoclonal Antibody [Clone ID: OTI4C5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01389-3)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI4C5
Isotype
IgG1
Immunogen
Full length human recombinant protein of human HK2 (NP_000180) produced in HEK293T cell.
Reactive Species
M01389-3 is reactive to HK2 in Dog, Human, Mouse, Rat
Calculated molecular weight
102.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01389-3 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IF: 1:100
Flow cytometry: 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HK2.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HK2 monoclonal antibody.
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HEK293T cells transfected with either pCMV6-ENTRY HK2 (Red) or empty vector control plasmid (Blue) were immunostained with anti-HK2 mouse monoclonal (M01389-3)
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Anti-HK2 mouse monoclonal antibody (M01389-3) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HK2.
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Immunofluorescent staining of HepG2 cells using anti-HK2 mouse monoclonal antibody (M01389-3).
Specific Publications For Anti-Hexokinase II (HK2) Mouse Monoclonal Antibody [Clone ID: OTI4C5] (M01389-3)
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