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- Table of Contents
HK2 antibody (A01389) performs well in Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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Flow Cytometry analysis of HepG2 cells using anti-Hexokinase II antibody (A01389).
Overlay histogram showing HepG2 cells stained with A01389 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hexokinase II Antibody (A01389, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HepG2 cells stained with A01389 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hexokinase II Antibody (A01389, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Hexokinase II/HK2 Antibody Picoband®
HK2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IHC, WB |
Tested Applications
HK2 antibody was positively detected by WB in:
human Hela whole cell, human MCF-7 whole cell, human HepG2 whole cell, human Hela- WT whole cell, human Hela-HK2 KO whole cell, rat skeletal muscle tissue, rat heart tissue, mouse skeletal muscle tissue, mouse heart tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HK2 antibody was positively detected by IHC in:
human breast cancer tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
HK2 antibody was positively detected by FC in:
HepG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
Flow Cytometry(Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Hexokinase II/HK2 Antibody Picoband®
View all HK2 Antibodies
View all HK2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Hexokinase II, different from the related mouse and rat sequences by four amino acids.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
102 kDa
Calculated Molecular Weight
102.4 kDa
Background of HK2
Hexokinase 2, also known as HK2, is an enzyme which in humans is encoded by the HK2 gene on chromosome 2. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
Cite This Product
Anti-Hexokinase II/HK2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01389)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Mouse
89%
Moderate Homology
Rat
89%
Moderate Homology
Rabbit
89%
Moderate Homology
Cow
87%
Moderate Homology
Sheep
84%
Moderate Homology
Dog
82%
Moderate Homology
Zebrafish
63%
Low homology
Drosophila
8%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for HK2 antibody
Immunohistochemistry (IHC) protocol for HK2 antibody
Flow Cytometry (FC) protocol for HK2 antibody
Publications
Application:
Species:
The fluoroquinoline compound exerts anti-erythroleukemic effects by dual-targeting GLUT1 and the PI3K/AKT signaling pathway
Published: 2026-03-27
Journal: Scientific Reports
Application: WB
Impact Factor: 3.90
Species: Human
PMID: 41896383
HCMV immediate-early protein IE2 induces neurotoxicity and hearing loss by disrupting TSC2-mTOR signaling and metabolic homeostasis
Published: 2025-12-02
Journal: Journal of Neuroinflammation
Application: WB
Impact Factor: 10.10
Species: Mouse
PMID: 41331812
Mitochondrial Genome-Encoded lncND5 Regulates Mitophagy in Hypoxic Pulmonary Artery Smooth Muscle Cell
Published: 2025-05-14
Journal: FASEB JOURNAL
Impact Factor: 4.20
PMID: 40364724
Infused juice concentrate of Japanese plum Prunus mume attenuates inflammatory vascular remodeling in a mouse model of hypertension induced by angiotensin II
Published: 2023-06-13
Journal: HYPERTENSION RESEARCH
Application: WB
Impact Factor: 5.40
Species: Rat
PMID: 37308550
Glucose consumption of vascular cell types in culture: toward optimization of experimental conditions
Published: 2022-01-12
Journal: AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
Application: WB
Impact Factor: 5.28
Species: Rat
PMID: 34817269
CircPOSTN/miR-361-5p/TPX2 axis regulates cell growth, apoptosis and aerobic glycolysis in glioma cells
Published: 2020-08-06
Journal: Cancer Cell International
Application: WB
Impact Factor: 4.18
Species: Human
PMID: 32774168
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Product Promise
We promise Anti-Hexokinase II/HK2 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01389
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026