Anti-HICE1 HAUS8 Antibody

Haus8 antibody

Boster Bio Anti-HICE1 HAUS8 Antibody (Catalog # A12994). Tested in ELISA, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A12994
Size: 100uL
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-HICE1 HAUS8 Antibody

View all Haus8 Antibodies

SKU/Catalog Number

A12994

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-HICE1 HAUS8 Antibody (Catalog # A12994). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-HICE1 HAUS8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12994)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

Anti-HICE1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant full length Hice1 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12994 is reactive to Haus8 in Human

Applications

A12994 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

41.653kDa

Background of Haus8

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Hice1 contributes to the mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Normal bipolar spindle formation is critical for accurate chromosome segregation and proper mitotic progression. Failure in this event leads to spindle checkpoint activation and chromosome missegregation that ultimately leads to aneuploidy. Hice1 binds to microtubules directly, and promotes spindle integrity and chromosome stability. Hice1 has also shown to play an important role in targeting the gamma TuRC complex to the mitotic spindle, a step that appears to be required for spindle-mediated microtubule generation and normal chromosome segregation. The HAUS augmin-like complex's interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. During interphase, it is primarily cytoplasmic, associating with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, it additionally associates with the spindle midzone and midbody, respectively. Further characterization of the function of Hice1 will likely be important for better understanding the mechanism of normal mitotic progression and high fidelity chromosome segregation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:250,000
WB: 1:10,000

Validation Images & Assay Conditions

Gene/Protein Information For Haus8 (Source: Uniprot.org, NCBI)

Gene Name

Haus8

Full Name

HAUS augmin-like complex subunit 8

Weight

41.653kDa

Superfamily

HAUS8 family

Alternative Names

DGT4; HAUS Augmin Like Complex Subunit 8; HEC1/NDC80 interacting, centrosome associated 1; HEC1/NDC80-Interacting Centrosome-Associated Protein 1; Hec1-Interacting And Centrosome-Associated 1; HICE1; NY-SAR-48; Sarcoma Antigen NY-SAR-48

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Haus8, check out the Haus8 Infographic

Haus8 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Haus8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-HICE1 HAUS8 Antibody

Question

I have attached the WB image, lot number and protocol we used for cervix carcinoma using anti-HICE1 antibody A12994. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-09

Question

We are currently using anti-HICE1 antibody A12994 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-18

Answer

The anti-HICE1 antibody (A12994) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-18

Question

Does A12994 anti-HICE1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

It shows on the product datasheet, A12994 anti-HICE1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-17

Question

Is there a BSA free version of anti-HICE1 antibody A12994 available?

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HICE1 antibody A12994 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-20

Question

I was wanting to use your anti-HICE1 antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

It shows on the product datasheet, A12994 anti-HICE1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-03

Order DetailsPrice
A12994-100uL

100uL

$599

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