Product Info Summary
SKU: | A12994 |
---|---|
Size: | 100uL |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-HICE1 HAUS8 Antibody
SKU/Catalog Number
A12994
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-HICE1 HAUS8 Antibody (Catalog # A12994). Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-HICE1 HAUS8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12994)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
Anti-HICE1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant full length Hice1 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12994 is reactive to Haus8 in Human
Applications
A12994 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
41.653kDa
Background of Haus8
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Hice1 contributes to the mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Normal bipolar spindle formation is critical for accurate chromosome segregation and proper mitotic progression. Failure in this event leads to spindle checkpoint activation and chromosome missegregation that ultimately leads to aneuploidy. Hice1 binds to microtubules directly, and promotes spindle integrity and chromosome stability. Hice1 has also shown to play an important role in targeting the gamma TuRC complex to the mitotic spindle, a step that appears to be required for spindle-mediated microtubule generation and normal chromosome segregation. The HAUS augmin-like complex's interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. During interphase, it is primarily cytoplasmic, associating with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, it additionally associates with the spindle midzone and midbody, respectively. Further characterization of the function of Hice1 will likely be important for better understanding the mechanism of normal mitotic progression and high fidelity chromosome segregation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:250,000
WB: 1:10,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of HICE1 expression in right lane (HICE1i): lysates from sh-HICE1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control.. HICE1 at 45KD was detected using rabbit anti-HICE1 Antigen Affinity purified polyclonal antibody (Catalog # A12994) at 1:10000. Personal communication, Kyung S. Lee, NCI, Bethesda, MD.
Protein Target Info & Infographic
Gene/Protein Information For Haus8 (Source: Uniprot.org, NCBI)
Gene Name
Haus8
Full Name
HAUS augmin-like complex subunit 8
Weight
41.653kDa
Superfamily
HAUS8 family
Alternative Names
DGT4; HAUS Augmin Like Complex Subunit 8; HEC1/NDC80 interacting, centrosome associated 1; HEC1/NDC80-Interacting Centrosome-Associated Protein 1; Hec1-Interacting And Centrosome-Associated 1; HICE1; NY-SAR-48; Sarcoma Antigen NY-SAR-48
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Haus8, check out the Haus8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Haus8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HICE1 HAUS8 Antibody (A12994)
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No publications found for A12994
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-HICE1 HAUS8 Antibody
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma using anti-HICE1 antibody A12994. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-09
Question
We are currently using anti-HICE1 antibody A12994 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
The anti-HICE1 antibody (A12994) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-18
Question
Does A12994 anti-HICE1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
It shows on the product datasheet, A12994 anti-HICE1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-17
Question
Is there a BSA free version of anti-HICE1 antibody A12994 available?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HICE1 antibody A12994 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-20
Question
I was wanting to use your anti-HICE1 antibody for WB for human cervix carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
It shows on the product datasheet, A12994 anti-HICE1 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-03