Human TLR3/Toll-like receptor 3 ELISA Kit PicoKine™

Human TLR3/Toll-like receptor 3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TLR3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0907
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human TLR3/Toll-like receptor 3 ELISA Kit PicoKine™

See all TLR3 products

SKU/Catalog Number

EK0907

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TLR3/Toll-like receptor 3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TLR3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human TLR3/Toll-like receptor 3 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0907)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: K27-S711

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0907 is reactive to TLR3 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0907 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0907-CAP Anti-Human TLR3 Pre-coated 96-well strip microplate 1
EK0907-ST Human TLR3 Standard 2 vials, 10 ng/tube
EK0907-DA Human TLR3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0790.1740.2580.4070.6841.0561.5702.274

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TLR3, Dilution ratio of 1:1, concentration in serum is 47-125pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TLR3/Toll-like receptor 3 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2901771532730117335141
Standard deviation14.21125.74292.9818.96152.5374.9%9
CV (%)4.9%7.1%5.5%8.8%%8.8%7.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TLR3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12903072972932966.412.1%
Sample 217711687195517181782103.885.8%
Sample 353276029636252875751459.87.9%
*number of samples for each test n=16.

Gene/Protein Information For TLR3 (Source: Uniprot.Org, NCBI)

Uniprot ID

O15455

Gene ID

7098

Gene Name

TLR3

Full Name

Toll-like receptor 3

Weight

103.829kDa

Superfamily

Toll-like receptor family

Alternative Names

CD283 antigen; CD283; TLR3; toll-like receptor 3 TLR3 CD283, IIAE2 toll like receptor 3 toll-like receptor 3

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TLR3, check out the TLR3 Infographic

TLR3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TLR3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Human TLR3/Toll-like receptor 3 ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated TLR3 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TLR3 antigen?

J. Thompson

Verified customer

Asked: 2020-05-15

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-05-15

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis TLR3 using the EK0907 Human TLR3 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-04-12

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2020-04-12

Question

Q: Can TLR3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-11-30

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-11-30

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

K. Jackson

Verified customer

Asked: 2018-02-04

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-02-04

Question

Could I use this kit to test tissue extracts? Keyword: applications

Verified Customer

Verified customer

Asked: 2017-12-26

Answer

Human TLR3 ELISA Kit (EK0907) is for quantitative detection of human TLR3 in cell culture supernates, serum and plasma. We did not test tissue samples, but the kit can work if the concentration of target protein is in the test range of the kit. We suggest the customers to do a pilot experiment if they want to use the kit to test tissue samples. Please contact us for suggested tissue sample processing method.

Boster Scientific Support

Answered: 2017-12-26

Question

Q: how to analyze ELISA data? I measured TLR3 level in cell lysates.

Verified Customer

Verified customer

Asked: 2017-04-07

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2017-04-07

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of TLR3 in Human cell lysates? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2015-05-27

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TLR3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2015-05-27

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