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HIF1A antibody (PB9253) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 82 Publications

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pb9253 3 IHC anti hif 1 alpha picoband antibody

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IHC analysis of HIF 1 alpha using anti-HIF 1 alpha antibody (PB9253).
HIF 1 alpha was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HIF 1 alpha Antibody (PB9253) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Designing a Western blot for HIF1A? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the HIF1A Western Blot Guide

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Anti-HIF-1-alpha/HIF1A Antibody Picoband®

Name: Anti-HIF-1-alpha/HIF1A Antibody Picoband®
Brand: Boster Bio
SKU: PB9253
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

HIF1A antibody

82 Publications|

1 Review

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Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IHC, WB

Tested Applications

HIF1A antibody was positively detected by WB in:

human HepG2 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

HIF1A antibody was positively detected by IHC in:

mouse intestine tissue, rat intestine tissue, human intestinal cancer tissue

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Mouse

Tested

Recommend

Rat

Tested

Recommend

Monkey

Predicted

Rabbit

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Product Information

Product Name

Anti-HIF-1-alpha/HIF1A Antibody Picoband®
View all HIF1A Antibodies

Form

Lyophilized

Uniprot

Q16665

Gene ID

3091

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminal of human HIF-1-alpha, different from the related mouse and rat sequences by three amino acids.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

120 kDa

Calculated Molecular Weight

92.7 kDa

Background of HIF1A

HIF-1α (Hypoxia-inducible factor 1α, HIF1A) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced O2 availability in mammals, including angiogenesis, erythropoiesis and glycolysis. This gene was mapped to 14q21-q24. HIF-1α transactivate genes required for energy metabolism and tissue perfusion and is necessary for embryonic development and tumor explant growth. HIF-1alpha is over expressed during carcinogenesis, myocardial infarction and wound healing. It is crucial for the cellular response to hypoxia and is frequently over expressed in human cancers, resulting in the activation of genes essential for cell survival. HIF-1α regulates the survival and function in the inflammatory microenvironment directly. It is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability.

Cite This Product

Anti-HIF-1-alpha/HIF1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9253)

Protocols

Western Blot protocol for HIF1A antibody

Review Protocol

Immunohistochemistry (IHC) protocol for HIF1A antibody

Review Protocol

Publications

IDO1 Inhibitor RY103 Prevents Sepsis-Associated Immunosuppression in Cecal Ligation and Puncture Mice

Published: 2026-08-13

Journal: Phenomics

Application: WB

Impact Factor: 5.50

Species: Mouse

SKU	PB9253
Application	Western Blot
Sample	mouse 4T1 cells
Sample Processing Description	normal 4T1 cells, LPS-stimulated 4T1 cells, cells treated with low, medium, or high doses of the drug, and cells treated with a positive control drug.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody	HIF-1-alpha/HIF1A Antibody Picoband®
Primary Incubation	1:2500, overnight at 4 ℃
Secondary Antibody	HRP Goat Anti-Rabbit IgG
Secondary Incubation	1:10000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	With equal loading amounts, the HIF1A protein levels showed clear differences among the groups: the blank group had the lowest level, the model group the highest, and in the drug-treated groups, the levels decreased sequentially from low, medium, to high dose. The positive control group was comparable to the high-dose group.

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Anti-HIF-1-alpha/HIF1A Antibody Picoband®

HIF1A antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

IDO1 Inhibitor RY103 Prevents Sepsis-Associated Immunosuppression in Cecal Ligation and Puncture Mice

Effects of interstitial photodynamic therapy on transplanted tumors of human lung adenocarcinoma A549 cells in nude mice

Rational design of curcumin-borneol hybrids for rheumatoid arthritis pathological intervention via AKT/mTOR/HIF-1α pathway modulation

Anmei decoction alleviates myocardial injury in sleep-deprived rats by PI3K/Akt/mTOR/HIF-1 pathway

Hungatella hathewayi: A Tumor-Derived Bacterium Enriched in Colorectal Cancer Tissues and a Potential Diagnostic Biomarker

Babaodan suppresses inflammatory and vascular microenvironment in triple-negative breast cancer by inhibiting the cGAS-STING/NF-κB signaling pathway

Bifunctional Artificial Enzymes-Loaded Microgels With LOX- and CAT-Like Activities for Metabolic Reprogramming and Scarless Wound Repair

Hypoxia Imaging in Hepatic Fibrosis: A Preliminary Application of CAIX-Targeted MRI Probe in Rats

Hypoxia-induced secretory autophagy in cancer-associated fibroblasts promotes ECM remodelling through serglycin secretion in oral squamous cell carcinoma

DNMT3A-dependent suppression of HIF-1α alleviates myopia by reducing scleral ferroptosis and fibroblast extracellular matrix remodeling

Deferoxamine improves femoral trochlear dysplasia following patellar dislocation through HIF-1α/VEGF-mediated angiogenesis-osteogenesis coupling

Biomimetic Nanoplatform-Mediated CRISPR/Cas9 Delivery for Dual-Pathway Metabolic Blockade in Head and Neck Squamous Cell Carcinoma

Chlorella-derived natural photosynthetic system for in situ energy metabolism enhancement in cardiomyocytes

Comprehensive analysis and validation of angiogenesis in vascular dementia from the perspective of diagnosis, prevention, and treatment

Investigation of the molecular mechanism of Xiaoluo Wan in thyroid-associated ophthalmopathy: network analysis and in vivo study

Hypoxia inducible factor-1alpha expression correlates with inflammatory injury of blood-brain barrier which influences perihaematomal edema after intracerebral hemorrhage

Assessment of hypoxia status in a rat chronic liver disease model using IVIM and T1 mapping

Rod-Shaped Microgel Scaffolds with Interconnective Pores and Oxygen-generating Functions Promote Skin Wound Healing and Alleviate Hypertrophic Scar Formation

Zhishi Xiebai Guizhi Decoction modulates hypoxia and lipid toxicity to alleviate pulmonary vascular remodeling of pulmonary hypertension in rats

HIF-1α expression is associated with the pathological response to neoadjuvant chemotherapy in pancreatic ductal adenocarcinoma patients and can be predicted using CECT features

Phillygenin regulates the colorectal cancer tumor microenvironment by inhibiting hypoxia-inducible factor 1 alpha

Correlation between CT spectral quantitative parameters and expression levels of HIF-1α and ALX1 in non-small cell lung cancer

Nicotinamide Mononucleotide (NMN) Ameliorates Free Fatty Acid-Induced Pancreatic β-Cell Dysfunction via the NAD+/AMPK/SIRT1/HIF-1α Pathway

Prognostic implications of TOR1B expression across cancer types: a focus on basal-like breast cancer and cellular adaptations to hypoxia

Inhibition of Notch3/Hey1 ameliorates peribiliary hypoxia by preventing hypertrophic hepatic arteriopathy in biliary atresia progression

Hydrogen Attenuates Chronic Intermittent Hypoxia-Induced Cardiac Hypertrophy by Regulating Iron Metabolism

Remodeling of the liver fibrosis microenvironment based on nilotinib-loaded multicatalytic nanozymes with boosted antifibrogenic activity

Effect of RNAi-Mediated Survivin and Hypoxia-Inducible Factor 1α Gene Silencing on Proliferation, Invasion, Migration and Apoptosis of Gastric Cancer BGC-823 Cells

Xiaojianzhong decoction prevents gastric precancerous lesions in rats by inhibiting autophagy and glycolysis in gastric mucosal cells

Combinatorial treatment with Silybum marianum essential oil enhances the therapeutic efficacy of a 5-fluorouracil base therapy for hepatocellular carcinoma

Role of hypoxia-inducible factor-1α and survivin in enhancing radiosensitivity of breast cancer

Effects of lactate on metabolism and differentiation of CD4+T cells

Genetically engineered nano-melittin vesicles for multimodal synergetic cancer therapy

Iron supplementation inhibits hypoxia-induced mitochondrial damage and protects zebrafish liver cells from death

Deacetylation mechanism and potential reversal strategy of long QT syndrome on hERG K+ channel under hypoxia

Combined HIF-1α and SHH Up-Regulation Is a Potential Biomarker to Predict Poor Prognosis in Postoperative Hepatocellular Carcinoma

miR-138-5p Inhibits Vascular Mimicry by Targeting the HIF-1α/VEGFA Pathway in Hepatocellular Carcinoma

Protective Effect of Brassica rapa Polysaccharide against Acute High-Altitude Hypoxia-Induced Brain Injury and Its Metabolomics

Efficient starvation therapy with three-pathway blocking in combination with PTT/CDT for TME reversal and tumor apoptosis

Inactivation of mouse transmembrane prolyl 4-hydroxylase increases blood brain barrier permeability and ischemia-induced cerebral neuroinflammation

A NO/ROS/RNS cascaded-releasing nano-platform for gas/PDT/PTT/immunotherapy of tumors

AhR activation attenuates calcium oxalate nephrocalcinosis by diminishing M1 macrophage polarization and promoting M2 macrophage polarization

A Comparison of Ramipril and Bevacizumab to Mitigate Radiation-Induced Brain Necrosis: An Experimental Study

Engineering HepG2 spheroids with injectable fiber fragments as predictable models for drug metabolism and tumor infiltration

The study of effect and mechanism of 630-nm laser on human lung adenocarcinoma cell xenograft model in nude mice mediated by hematoporphyrin derivatives

Deletion of narfl leads to increased oxidative stress mediated abnormal angiogenesis and digestive organ defects in zebrafish

Propionate relieves pentylenetetrazol-induced seizures, consequent mitochondrial disruption, neuron necrosis and neurological deficits in mice

MiR-203a-3p inhibits retinal angiogenesis and alleviates proliferative diabetic retinopathy in oxygen-induced retinopathy (OIR) rat model via targeting VEGFA and HIF-1α

The effect of BimEL on gill remodeling of goldfish (Carassius auratus)

Role of the HIF‑1 signaling pathway in chronic obstructive pulmonary disease

HER2 and Src co-regulate proliferation, migration and transformation by downstream signaling pathways in arsenite-treated human uroepithelial cells

Resolvin D2 Limits Secondary Tissue Necrosis After Burn Wounds in Rats

Comparison of the expression of TGF‑β1, E‑cadherin, N‑cadherin, TP53, RB1CC1 and HIF‑1α in oral squamous cell carcinoma and lymph node metastases of humans and mice

Effect of synaptic adhesion-like molecule 3 on epileptic seizures: Evidence from animal models

Zebrafish let-7b acts downstream of hypoxia-inducible factor-1α to assist in hypoxia-mediated cell proliferation and cell cycle regulation

CD47 deficiency in tumor stroma promotes tumor progression by enhancing angiogenesis

Exposure to concentrated ambient fine particulate matter disrupts vascular endothelial cell barrier function via the IL-6/HIF-1α signaling pathway

Hypoxic conditioned medium of placenta-derived mesenchymal stem cells protects against scar formation

Role of hypoxia-inducible factor-1α in pathogenesis and disease evaluation of ulcerative colitis

Nitric oxide signaling pathway activation inhibits the immune escape of pancreatic carcinoma cells

2-Methoxyestradiol synergizes with sorafenib to suppress hepatocellular carcinoma by simultaneously dysregulating hypoxia-inducible factor-1 and -2

Sp1 modification of human endothelial nitric oxide synthase promoter increases the hypoxia-stimulated activity

Inactivation of Vhl in Osteochondral Progenitor Cells Causes High Bone Mass Phenotype and Protects Against Age-Related Bone Loss in Adult Mice

Arsenic induces the expressions of angiogenesis-related factors through PI3K and MAPK pathways in SV-HUC-1 human uroepithelial cells

Expression of Rac1, HIF-1α, and VEGF in Gastric Carcinoma: Correlation with Angiogenesis and Prognosis

Expression of hypoxia-inducible factor 1 alpha and oligodendrocyte lineage gene-1 in cultured brain slices after oxygen-glucose deprivation

How does hypoxia inducible factor-1α participate in enhancing the glycolysis activity in cervical cancer?