Data & Images
|Product Name||Anti-HIF1 Beta Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Aryl hydrocarbon receptor nuclear translocator(ARNT) detection. Tested with WB in Human.|
|Cite This Product||Anti-HIF1 Beta Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9129)|
|Replacement Item||This antibody may replace the following items: sc-161853|sc-161854|sc-17811|sc-17811-X|sc-17812|sc-17812-X|sc-271801|sc-271801-X|sc-366098|sc-393613|sc-393613-X|sc-393683|sc-393683-X|sc-393916|sc-55526|sc-5580|sc-5580-X|sc-5581|sc-5581-X|sc-56620|sc-65639|sc-70383|sc-8076|sc-8076-X|sc-8077|sc-8077-X|sc-8078|sc-8078-X from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||E.coli-derived human HIF1 beta recombinant protein (Position: V416-E789). Human HIF1 beta shares 86% and 83% amino acid (aa) sequences identity with mouse and rat HIF1 beta, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Aryl hydrocarbon receptor nuclear translocator|
|Molecular Weight||86636 MW|
|Protein Function||Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.|
|Sequence Similarities||Contains 1 bHLH (basic helix-loop-helix) domain.|
|Alternative Names||Aryl hydrocarbon receptor nuclear translocator;ARNT protein;Class E basic helix-loop-helix protein 2;bHLHe2;Dioxin receptor, nuclear translocator;Hypoxia-inducible factor 1-beta;HIF-1-beta;HIF1-beta;ARNT;BHLHE2;|
|Research Areas|||neuroscience|neurology process|neural signal transduction| epigenetics and nuclear signaling|transcription|polymerase associated factors|pol ii transcription|transcription factors| cancer|cancer metabolism|response to hypoxia| metabolism|pathways and processes|metabolism processes|types of disease||
Background for Aryl hydrocarbon receptor nuclear translocator
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-HIF1 Beta Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-HIF1 Beta Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti HIF1 beta (PB9129) at 0.5ug/ml
WB: Recombinant Human HIF1 beta Protein 0.5ng
Predicted bind size: 49KD
Observed bind size: 49KD
All lanes: Anti HIF1 beta (PB9129) at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: Jurkat Whole Cell Lysate at 40ug
Lane 4: U87 Whole Cell Lysate at 40ug
Lane5: Colo320 Whole Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 87KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,