Product Info Summary
SKU: | A00396-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-hnRNP A2B1/HNRNPA2B1 Antibody Picoband™
View all hnRNP A2B1 Antibodies
SKU/Catalog Number
A00396-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-hnRNP A2B1/HNRNPA2B1 Antibody Picoband™ catalog # A00396-1. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-hnRNP A2B1/HNRNPA2B1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00396-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human hnRNP A2B1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00396-1 is reactive to HNRNPA2B1 in Human, Mouse, Rat
Applications
A00396-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
37 kDa
Calculated molecular weight
37.43kDa
Background of hnRNP A2B1
Heterogeneous nuclear ribonucleoproteins A2/B1 is a protein that in humans is encoded by the HNRNPA2B1 gene. This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. This gene has been described to generate two alternatively spliced transcript variants which encode different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot,0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of hnRNP A2B1 using anti-hnRNP A2B1 antibody (A00396-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Hacat whole cell lysates,
Lane 3: human RT4 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: human SH-SY5Y whole cell lysates,
Lane 6: human U2OS whole cell lysates,
Lane 7: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-hnRNP A2B1 antigen affinity purified polyclonal antibody (Catalog # A00396-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for hnRNP A2B1 at approximately 37 kDa. The expected band size for hnRNP A2B1 is at 37 kDa.
Click image to see more details
Figure 2. IHC analysis of hnRNP A2B1 using anti-hnRNP A2B1 antibody (A00396-1).
hnRNP A2B1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-hnRNP A2B1 Antibody (A00396-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of hnRNP A2B1 using anti-hnRNP A2B1 antibody (A00396-1).
hnRNP A2B1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-hnRNP A2B1 Antibody (A00396-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of hnRNP A2B1 using anti-hnRNP A2B1 antibody (A00396-1).
hnRNP A2B1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-hnRNP A2B1 Antibody (A00396-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IF analysis of hnRNP A2B1 using anti-hnRNP A2B1 antibody (A00396-1).
hnRNP A2B1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-hnRNP A2B1 Antibody (A00396-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 6. Flow Cytometry analysis of A431 cells using anti-hnRNP A2B1 antibody (A00396-1).
Overlay histogram showing A431 cells stained with A00396-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-hnRNP A2B1 Antibody (A00396-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For HNRNPA2B1 (Source: Uniprot.org, NCBI)
Gene Name
HNRNPA2B1
Full Name
Heterogeneous nuclear ribonucleoproteins A2/B1
Weight
37.43kDa
Alternative Names
DKFZp779B0244; FLJ22720; heterogeneous nuclear ribonucleoprotein A2/B1; heterogeneous nuclear ribonucleoprotein B1; heterogeneous nuclear ribonucleoproteins A2/B1; hnRNP A2 / hnRNP B1; HNRNPA2; HNRNPB1; HNRPA2; HNRPA2B1hnRNP A2/B1; HNRPB1; nuclear ribonucleoprotein particle A2 protein; RNPA2; SNRPB1 HNRNPA2B1 HNRNPA2, HNRNPB1, HNRPA2, HNRPA2B1, HNRPB1, IBMPFD2, RNPA2, SNRPB1 heterogeneous nuclear ribonucleoprotein A2/B1 heterogeneous nuclear ribonucleoproteins A2/B1|HNRNPA2B1/MYC fusion|epididymis secretory sperm binding protein|hnRNP A2 / hnRNP B1|nuclear ribonucleoprotein particle A2 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HNRNPA2B1, check out the HNRNPA2B1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HNRNPA2B1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-hnRNP A2B1/HNRNPA2B1 Antibody Picoband™ (A00396-1)
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Customer Q&As
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1 Customer Q&As for Anti-hnRNP A2B1/HNRNPA2B1 Antibody Picoband™
Question
We are currently using anti-hnRNP A2B1/HNRNPA2B1 antibody A00396-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
The anti-hnRNP A2B1/HNRNPA2B1 antibody (A00396-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-13