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Product Info Summary
| SKU: | A03822 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-NIK MAP3K14 Antibody
SKU/Catalog Number
A03822
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-NIK MAP3K14 Antibody (Catalog # A03822). Tested in ELISA, WB, IF, ICC applications. This antibody reacts with Human.
Storage & Handling
NIK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-NIK MAP3K14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03822)
Host
Rabbit
Contents
NIK Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
NIK antibody was raised against a 17 amino acid peptide near the carboxy terminus of human NIK. The immunogen is located within the last 50 amino acids of NIK.
Reactive Species
A03822 is reactive to MAP3K14 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
104.0 kDa
Background of MAP3K14
Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα, LPS and mitogens. A serine/threonine protein kinase which mediates NF-κB activation by IL-1, TNFα and CD95 was identified recently and designated NIK (for NF-κB inducing kinase). NIK is an activator of IκB kinase alpha and beta (IKKα and IKKβ). Therefore, NIK is a key molecule in the NF-κB signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03822 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
NIK antibody can be used for detection of NIK by Western blot at 1 - 2 μg/mL.
Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Immunocytochemistry of NIK in Hek293 cells with NIK antibody at 10 μg/mL.
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Immunofluorescence of NIK in Hek293 cells with NIK antibody at 20 μg/mL.
Green: NIK Antibody (A03822)
Blue: DAPI staining
Specific Publications For Anti-NIK MAP3K14 Antibody (A03822)
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