|Validated Species:||Human, Mouse, Rabbit, Rat|
|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-HSP27 Monoclonal Antibody|
|Description||Mouse IgG monoclonal antibody for HSP27, heat shock 27kDa protein 1 (HSPB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;mouse;rat;rabbit. No cross reactivity with other proteins.|
|Cite This Product||Anti-HSP27 Monoclonal Antibody IAP-9 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1048)|
|Replacement Item||This antibody may replace the following items: sc-53718 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rabbit, Rat|
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Partially purified inhibitor of actin polymerization(IAP) protein from turkey gizzard smooth muscle.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Heat shock protein beta-1|
|Molecular Weight||22893 MW|
|Protein Function||Involved in stress resistance and actin organization.|
|Tissue Specificity||Expressed in a variety of tissues. High levels in lung, adrenal, xiphoid, adipose tissue, heart and striated and smooth muscle, lower levels in the CNS. Adult levels are much higher in the slow-twitch soleus muscle than in the fast-twitch rectus femoris and extensor digitorum muscles. .|
|Sequence Similarities||Belongs to the small heat shock protein (HSP20) family.|
|Subcellular Localization||Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles (By similarity). .|
|Alternative Names||Heat shock protein beta-1;HspB1;Heat shock 27 kDa protein;HSP 27;Hspb1;Hsp27;|
|Research Areas|||signal transduction|protein trafficking|chaperones|heat shock proteins| cancer|tumor biomarkers| cardiovascular|atherosclerosis|ischemia / reperfusion|heart|contractility|contractile proteins|actins||
Background for Heat shock protein beta-1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-HSP27 Monoclonal Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rabbit, rat, By Heat
Immunocytochemistry , 1μg/ml, Human, mouse, rabbit, rat, -
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rabbit, rat, -
Western blot, 0.5-2μg/ml, Human, mouse, rabbit, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.