|Validated Species:||Human, Mouse, Rabbit, Rat|
|Application:||IHC, ICC, WB|
Data & Images
|Product Name||Anti-HSP27 Monoclonal Antibody|
|Description||Mouse IgG monoclonal antibody for HSP27, heat shock 27kDa protein 1 (HSPB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;mouse;rat;rabbit. No cross reactivity with other proteins.|
|Cite This Product||Anti-HSP27 Monoclonal Antibody IAP-9 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1048)|
|Replacement Item||This antibody may replace the following items: sc-53718 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rabbit, Rat|
|Application||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Partially purified inhibitor of actin polymerization(IAP) protein from turkey gizzard smooth muscle.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Heat shock protein beta-1|
|Molecular Weight||22893 MW|
|Protein Function||Involved in stress resistance and actin organization.|
|Tissue Specificity||Expressed in a variety of tissues. High levels in lung, adrenal, xiphoid, adipose tissue, heart and striated and smooth muscle, lower levels in the CNS. Adult levels are much higher in the slow-twitch soleus muscle than in the fast-twitch rectus femoris and extensor digitorum muscles. .|
|Sequence Similarities||Belongs to the small heat shock protein (HSP20) family.|
|Subcellular Localization||Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles (By similarity). .|
|Alternative Names||Heat shock protein beta-1;HspB1;Heat shock 27 kDa protein;HSP 27;Hspb1;Hsp27;|
|Research Areas|||signal transduction|protein trafficking|chaperones|heat shock proteins| cancer|tumor biomarkers| cardiovascular|atherosclerosis|ischemia / reperfusion|heart|contractility|contractile proteins|actins||