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Product Info Summary
|Reactive Species:||Human, Mouse, Rabbit, Rat|
|Application:||IHC, ICC, WB|
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Anti-HSP27 Hspb1 Monoclonal Antibody
Boster Bio Anti-HSP27 Hspb1 Monoclonal Antibody catalog # MA1048. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSP27 Hspb1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1048)
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross-reactivity with other proteins
MA1048 is reactive to Hspb1 in Human, Mouse, Rabbit, Rat
MA1048 is guaranteed for IHC, ICC, WB Boster Guarantee
Background of HSP27
The heat-shock proteins (HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Heat-shock 27-kD protein1also knows as HSPB. Synthesis of the small (27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. HSP27 gene is mapped to 7q11.23. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rabbit, rat, By Heat
Immunocytochemistry , 1μg/ml, Human, mouse, rabbit, rat, -
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rabbit, rat, -
Western blot, 0.5-2μg/ml, Human, mouse, rabbit, rat
Validation Images & Assay Conditions
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Boster Kit Box
Gene/Protein Information For Hspb1 (Source: Uniprot.org, NCBI)
Heat shock protein beta-1
small heat shock protein (HSP20) family
28 kDa heat shock protein; DKFZp586P1322; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; heat shock protein beta-1; HMN2B; HS.76067; HSP25; HSP27; HSP27HSP 27; HSP28CMT2F; HSPB1; SRP27; Stress-responsive protein 27 Hspb1|Hsp25, Hsp27|heat shock protein family B (small) member 1|heat shock protein beta-1|HSP 27|heat shock 27 kDa protein 1|heat shock 27kDa protein 1|heat shock protein B1*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on Hspb1, check out the Hspb1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Hspb1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
MA1048 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wu Yw, Hao T, Kou Xx, Gan Yh, Ma Xc. Arch Oral Biol. 2015 Sep;60(9):1310-8. Doi: 10.1016/J.Archoralbio.2015.05.011. Epub 2015 Jun 11. Synovial Trpv1 Is Upregulated By 17-??-Estradiol And Involved In Allodynia Of Inflamed Temporomandibular Joints I...
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