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Product Info Summary
| SKU: | MA1048 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rabbit, Rat |
| Host: | Mouse |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-HSP27 Hspb1 Monoclonal Antibody
SKU/Catalog Number
MA1048
BM1586 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HSP27 Hspb1 Monoclonal Antibody catalog # MA1048. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSP27 Hspb1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1048)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
IAP-9
Isotype
Mouse IgG1
Immunogen
Partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1048 is reactive to Hspb1 in Human, Mouse, Rabbit, Rat
Observed Molecular Weight
50 kDa
Calculated molecular weight
22.9 kDa
Background of Hspb1
The heat-shock proteins (HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Heat-shock 27-kD protein1also knows as HSPB. Synthesis of the small (27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. HSP27 gene is mapped to 7q11.23. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1048 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rabbit, rat
Immunocytochemistry , 1μg/ml, Human, mouse, rabbit, rat, -
Immunohistochemistry (Frozen Section), 1-2μg/ml, Human, mouse, rabbit, rat, -
Western blot, 0.5-2μg/ml, Human, mouse, rabbit, rat
Validation Images & Assay Conditions
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Boster Kit Box
Specific Publications For Anti-HSP27 Hspb1 Monoclonal Antibody (MA1048)
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