Anti-HSP27 Hspb1 Monoclonal Antibody
Mouse IgG monoclonal antibody for HSP27, heat shock 27kDa protein 1 (HSPB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;mouse;rat;rabbit. No cross reactivity with other proteins. Cited in 1 publication(s).
Anti-HSP27 Hspb1 Monoclonal Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rabbit, Rat|
|Applications:||IHC, ICC, WB|
|Product Name||Anti-HSP27 Hspb1 Monoclonal Antibody
See all HSP27 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Monoclonal clone# G2 antibody for HSP27/HSPB1 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. HSP27/HSPB1 information: Molecular Weight: 22893 MW; Subcellular Localization: Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles (By similarity); Tissue Specificity: Expressed in a variety of tissues. High levels in lung, adrenal, xiphoid, adipose tissue, heart and striated and smooth muscle, lower levels in the CNS. Adult levels are much higher in the slow-twitch soleus muscle than in the fast-twitch rectus femoris and extensor digitorum muscles.|
|Cite This Product||Anti-HSP27 Hspb1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1048)|
|Similar Products From
|Anti-HSP27 Hspb1 Monoclonal Antibody may replace the following items: sc 53718.|
|Reactivity/Species||Human, Mouse, Rabbit, Rat|
|Applications||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||Partially purified inhibitor of actin polymerization(IAP) protein from turkey gizzard smooth muscle.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For Hspb1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||24471|
|Species Of This Entry||Rat|
|Protein Name||Heat shock protein beta-1|
|Superfamily||small heat shock protein (HSP20) family|
|Alternative Names||HSP27|28 kDa heat shock protein; DKFZp586P1322; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; heat shock protein beta-1; HMN2B; HS.76067; HSP25; HSP27; HSP27HSP 27; HSP28CMT2F; HSPB1; SRP27; Stress-responsive protein 27|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Dephosphorylation; Ubiquitination; Reduction; Oxidation; Methylation; Deacetylation; Acetylation; Demethylation; Sumoylation; Carboxylation; Hydroxylation; Myristoylation|
|Gene Location||12q12, on Chromasome 12, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Hspb1 (Source: Uniprot.org, NCBI)
|Protein Function||Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding- competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.|
|Tissue Specificity||Expressed in a variety of tissues. High levels in lung, adrenal, xiphoid, adipose tissue, heart and striated and smooth muscle, lower levels in the CNS. Adult levels are much higher in the slow-twitch soleus muscle than in the fast-twitch rectus femoris and extensor digitorum muscles.|
|Research Areas||Cancer; Cell Biology; Cell Cycle and Replication; Golgi Apparatus Markers; Membrane Trafficking and Chaperones; Neuroscience; Phospho-Specific|
|Related Diseases||Malignant Neoplasms; Neoplasms; Carcinoma; Ischemia; Inflammation; Neoplasm Metastasis; Malignant Neoplasm Of Breast; Mammary Neoplasms; Physiological Stress; Neurodegenerative Disorders; Adenocarcinoma; Tissue Adhesions; Charcot-marie-tooth Disease; Nervousness; Malignant Squamous Cell Neoplasm; Malignant Paraganglionic Neoplasm; Reperfusion Injury; Hypoxia|
|Related Pathways||Cell Death; Localization; Pathogenesis; Cell Cycle; Cell Proliferation; Cell Growth; Transport; Secretion; Drug Resistance; Cell Migration; Translation; Aging; Response To Heat; Cell Differentiation; Regeneration; Inflammatory Response; Response To Stress; Protein Folding; Neuroprotection; Autophagy|
|Background||The heat-shock proteins(HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Heat-shock 27-kD protein1also knows as HSPB. Synthesis of the small(27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. HSP27 gene is mapped to 7q11.23. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.|
|Scientific References||PMID: 8793069 by Welsh M.J., et al. Variation in expression of hsp27 messenger ribonucleic acid during the cycle of the seminiferous epithelium and co-localization of hsp27 and microfilaments in Sertoli cells of the rat.|
PMID: 7916612 by Uoshima K., et al. Isolation and characterization of a rat HSP 27 gene.
Our Boster Quality Guarantee for Anti-HSP27 Hspb1 Monoclonal Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rabbit, rat, By Heat
Immunocytochemistry , 1μg/ml, Human, mouse, rabbit, rat, -
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rabbit, rat, -
Western blot, 0.5-2μg/ml, Human, mouse, rabbit, rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
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Total number of citations: 1
|Wu Yw, Hao T, Kou Xx, Gan Yh, Ma Xc. Arch Oral Biol. 2015 Sep;60(9):1310-8. Doi: 10.1016/J.Archoralbio.2015.05.011. Epub 2015 Jun 11. Synovial Trpv1 Is Upregulated By 17-??-Estradiol And Involved In Allodynia Of Inflamed Temporomandibular Joints I...||PubMed ID 26117090|
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