radio
SKU MA1048
Size 100μg/vial
Reactivity Human, Mouse, Rabbit, Rat
Clonality Monoclonal
Clone Number IAP-9
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, ICC, WB

Overview

Product Name Anti-HSP27 Monoclonal Antibody
SKU/Catalog Number MA1048
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for HSP27, heat shock 27kDa protein 1 (HSPB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;mouse;rat;rabbit. No cross reactivity with other proteins.
Cite This Product Anti-HSP27 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1048)
Host Mouse
Immunogen Partially purified inhibitor of actin polymerization(IAP) protein from turkey gizzard smooth muscle.
Reactivity Human, Mouse, Rabbit, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rabbit, rat, By Heat
Immunocytochemistry , 1μg/ml, Human, mouse, rabbit, rat, -
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rabbit, rat, -
Western blot, 0.5-2μg/ml, Human, mouse, rabbit, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P42930
Gene Name Hspb1
Protein Name Heat shock protein beta-1
Tissue Specificity Expressed in a variety of tissues. High levels in lung, adrenal, xiphoid, adipose tissue, heart and striated and smooth muscle, lower levels in the CNS. Adult levels are much higher in the slow-twitch soleus muscle than in the fast-twitch rectus femoris and extensor digitorum muscles. .
Alternative Names Heat shock protein beta-1;HspB1;Heat shock 27 kDa protein;HSP 27;Hspb1;Hsp27;
Subcellular Localization Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles (By similarity). .
Molecular Weight 22893 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Involved in stress resistance and actin organization.
Background The heat-shock proteins(HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Heat-shock 27-kD protein1also knows as HSPB. Synthesis of the small(27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. HSP27 gene is mapped to 7q11.23. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.

Order Product (MA1048)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

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Publications

Wu Yw, Hao T, Kou Xx, Gan Yh, Ma Xc. Arch Oral Biol. 2015 Sep;60(9):1310-8. Doi: 10.1016/J.Archoralbio.2015.05.011. Epub 2015 Jun 11. Synovial Trpv1 Is Upregulated By 17-??-Estradiol And Involved In Allodynia Of Inflamed Temporomandibular Joints In...

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.