Anti-HSP60 Antibody (Monoclonal, LK1)
Mouse IgG monoclonal antibody for HSP60, heat shock 60kDa protein 1 (chaperonin) (HSPD1) detection. Tested with WB, IHC-P in Human;rat;chicken. No cross reactivity with other proteins.
|Reactivity||Chicken, Human, Rat|
|Ig Isotype||Mouse IgG1|
|Product Name||Anti-HSP60 Antibody (Monoclonal, LK1)
See all Hspd1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Monoclonal clone# G2 antibody for HSP60/HSPD1 detection. Host: Mouse.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Chicken. HSP60/HSPD1 information: Molecular Weight: 60955 MW; Subcellular Localization: Mitochondrion matrix .|
|Cite This Product||Anti-HSP60 Antibody (Monoclonal, LK1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1049)|
|Specificity||Anti-HSP60 Antibody (Monoclonal, LK1) (MA1049) reacts with Chicken, Human, Rat Hspd1, in native form and recombinant. Superfamily members of Hspd1 are not reactive to MA1049.|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Reconstitution||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Immunogen||Recombinant human heat shock protein 60(HSP60)|
|Reactivity||Chicken, Human, Rat|
Our Boster Quality Guarantee for Anti-HSP60 Antibody (Monoclonal, LK1) covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 4-8μg/ml, Human, chicken, rat, By Heat
Western blot, 2-4μg/ml, Human, chicken, rat
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-HSP60 Antibody (Monoclonal, LK1) (MA1049).Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
Images And Assay Conditions
Boster Kit Box
Protein Target Info (Source: Uniprot.org)
|Protein Name||60 kDa heat shock protein, mitochondrial|
|Alternative Names||60 kDa heat shock protein, mitochondrial;60 kDa chaperonin;Chaperonin 60;CPN60;HSP-65;Heat shock protein 60;HSP-60;Hsp60;Mitochondrial matrix protein P1;Hspd1;Hsp60;|
|Subcellular Localization||Mitochondrion matrix .|
|Molecular Weight||60955 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.|
|Research Areas||Chicken, Human, Rat
*You can search these to find other products in these research areas.
|Background||Heat shock 60KD protein(HSP60) is a member of the chaperonin class of protein factors and the nuclear encoded mitochondrial HSP60 is required for the assembly into oligomeric complexes of proteins imported into the mitochondrial matrix. HSP60 is linked head to head comprising approximately 17 kb and consist of 12 exons. HSP60 is a self-molecule, it can downregulate adaptive immune responses by upregulating Tregs innately through TLR2 signaling.|
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to [email protected]
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to hsp60 antibody, groel antibody