|Validated Species:||Chicken, Human, Rat|
Data & Images
|Product Name||Anti-HSP60 Antibody (Monoclonal, LK1)|
|Description||Mouse IgG monoclonal antibody for HSP60, heat shock 60kDa protein 1 (chaperonin) (HSPD1) detection. Tested with WB, IHC-P in Human;rat;chicken. No cross reactivity with other proteins.|
|Cite This Product||Anti-HSP60 Antibody (Monoclonal, LK1) LK1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1049)|
|Replacement Item||This antibody may replace the following items: sc-1052|sc-13115|sc-136291|sc-13966|sc-1722|sc-1722-R|sc-271215|sc-376240|sc-376261|sc-59567|sc-65568 from Santa Cruz Biotechnology.|
|Validated Species||Chicken, Human, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Recombinant human heat shock protein 60(HSP60)|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||60 kDa heat shock protein, mitochondrial|
|Molecular Weight||60955 MW|
|Protein Function||Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.|
|Sequence Similarities||Belongs to the chaperonin (HSP60) family.|
|Subcellular Localization||Mitochondrion matrix .|
|Alternative Names||60 kDa heat shock protein, mitochondrial;60 kDa chaperonin;Chaperonin 60;CPN60;HSP-65;Heat shock protein 60;HSP-60;Hsp60;Mitochondrial matrix protein P1;Hspd1;Hsp60;|
|Research Areas|||tags & cell markers|subcellular markers|organelles|mitochondria| signal transduction|protein trafficking|chaperones|heat shock proteins| kits/ lysates/ other|elisa kits|signal transduction proteins elisa kits| isotype/loading controls|hsp60||
Background for 60 kDa heat shock protein, mitochondrial
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-HSP60 Antibody (Monoclonal, LK1) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 4-8μg/ml, Human, chicken, rat, By Heat
Western blot, 2-4μg/ml, Human, chicken, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.