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| SKU | MA1049 |
|---|---|
| Size | 100μg/vial |
| Reactivity | Chicken, Human, Rat |
| Clonality | Monoclonal |
| Clone Number | LK1 |
| Host | Mouse |
| Ig Isotype | Mouse IgG1 |
| Applications | IHC, WB |
Overview
| Product Name | Anti-HSP60 Antibody (Monoclonal, LK1) |
|---|---|
| SKU/Catalog Number | MA1049 |
| Storage & Handling | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Size | 100μg/vial |
| Description | Mouse IgG monoclonal antibody for HSP60, heat shock 60kDa protein 1 (chaperonin) (HSPD1) detection. Tested with WB, IHC-P in Human;rat;chicken. No cross reactivity with other proteins. |
| Cite This Product | Anti-HSP60 Antibody (Monoclonal, LK1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1049) |
| Host | Mouse |
| Contents/Buffer | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Form | Lyophilized |
| Immunogen | Recombinant human heat shock protein 60(HSP60) |
| Reactivity | Chicken, Human, Rat |
Assay Details
Assay Dilutions Overview
Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 4-8μg/ml, Human, chicken, rat, By Heat
Western blot, 2-4μg/ml, Human, chicken, rat
Immunohistochemistry(Paraffin-embedded Section), 4-8μg/ml, Human, chicken, rat, By Heat
Western blot, 2-4μg/ml, Human, chicken, rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).Images And Assay Conditions
Boster Kit Box
Target Info
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P63039 |
|---|---|
| Gene Name | HSPD1 |
| Protein Name | 60 kDa heat shock protein, mitochondrial |
| Alternative Names | 60 kDa heat shock protein, mitochondrial;60 kDa chaperonin;Chaperonin 60;CPN60;HSP-65;Heat shock protein 60;HSP-60;Hsp60;Mitochondrial matrix protein P1;Hspd1;Hsp60; |
| Subcellular Localization | Mitochondrion matrix . |
| Molecular Weight | 60955 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. |
|---|---|
| Research Areas | Chaperones, Heat Shock Proteins, Mitochondria, Organelles, Protein Trafficking, Signal Transduction, Subcellular Markers, Tags & Cell Markers *You can search these to find other products in these research areas. |
| Background | Heat shock 60KD protein(HSP60) is a member of the chaperonin class of protein factors and the nuclear encoded mitochondrial HSP60 is required for the assembly into oligomeric complexes of proteins imported into the mitochondrial matrix. HSP60 is linked head to head comprising approximately 17 kb and consist of 12 exons. HSP60 is a self-molecule, it can downregulate adaptive immune responses by upregulating Tregs innately through TLR2 signaling. |
Other Recommended Resources
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Publications
Cui Yd, Du Yz, Lu Mx, Qiang Ck. J Insect Sci. 2010;10:100. Doi: 10.1673/031.010.10001. Cloning Of The Heat Shock Protein 60 Gene From The Stem Borer, Chilo Suppressalis, And Analysis Of Expression Characteristics Under Heat Stress.
Hager L, Li L, Pun H, Liu L, Hossain Ma, Maguire Gf, Naples M, Baker C, Magomedova L, Tam J, Adeli K, Cummins Cl, Connelly Pw, Ng Ds. J Biol Chem. 2012 Jun 8;287(24):20755-68. Doi: 10.1074/Jbc.M112.340919. Epub 2012 Apr 12. Lecithin:Cholesterol Ac...
Li L, Nan P, Zhai S, Wang L, Si S, Chang Z. Mol Cell Biochem. 2014 Feb;387(1-2):143-50. Doi: 10.1007/S11010-013-1879-0. Epub 2013 Nov 21. Molecular Cloning, Characterization, And Expression Of Hsp60 In Caudal Fin Regeneration Of Misgurnus Anguilli...
Li L, Zhai S, Wang L, Si S, Wu H, Chang Z. Fish Shellfish Immunol. 2014 Feb;36(2):401-8. Doi: 10.1016/J.Fsi.2013.12.016. Epub 2013 Dec 29. Hsp60 In Caudal Fin Regeneration From Paramisgurnus Dabryanus: Molecular Cloning And Expression Characteriza...
Customer Q&As
Q: Is your western blot protocol provided from the website applicable for all your antibodies?
Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody?
Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to hsp60 antibody, groel antibody