Click image to see more details
Product Info Summary
| SKU: | MA1049 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Chicken, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-HSP60 Hspd1 Antibody (Monoclonal, LK1)
SKU/Catalog Number
MA1049
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HSP60 Hspd1 Antibody (Monoclonal, LK1) catalog # MA1049. Tested in IHC-P, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSP60 Hspd1 Antibody (Monoclonal, LK1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1049)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
LK1
Isotype
Mouse IgG1
Immunogen
Recombinant human heat shock protein 60 (HSP60)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross Reactivity
No cross reactivity with other proteins
Reactive Species
MA1049 is reactive to Hspd1 in Chicken, Human, Mouse, Rat
Applications
MA1049 is guaranteed for IHC-P, WB Boster Guarantee
Background of HSP60
Heat shock 60KD protein (HSP60) is a member of the chaperonin class of protein factors and the nuclear encoded mitochondrial HSP60 is required for the assembly into oligomeric complexes of proteins imported into the mitochondrial matrix. HSP60 is linked head to head comprising approximately 17 kb and consist of 12 exons. HSP60 is a self-molecule, it can downregulate adaptive immune responses by upregulating Tregs innately through TLR2 signaling.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
Submit A Review
Assay dilution & Images
Reconsitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 2-4μg/ml, Human, chicken, rat
Immunohistochemistry (Paraffin-embedded Section), 4-8μg/ml, Human, chicken, mouse, rat, By Heat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of HSP60 using anti-HSP60 antibody (MA1049).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSP60 antigen affinity purified monoclonal antibody (Catalog # MA1049) at 2 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSP60 at approximately 65 kDa. The expected band size for HSP60 is at 65 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Hspd1 (Source: Uniprot.Org, NCBI)
Uniprot ID
P63039
Gene ID
63868
Gene Name
Hspd1
Full Name
60 kDa heat shock protein, mitochondrial
Weight
60.955kDa
Superfamily
chaperonin (HSP60) family
Alternative Names
60 kDa chaperonin; Chaperonin 60; cpn60; GROEL; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1 (chaperonin); Heat shock protein 60; heat shock protein 65; HLD4; HSP60; HSP-60; HSP60SPG13; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; spastic paraplegia 13 (autosomal dominant); SPG13 Hspd1|Hsp60-30p, Hspd1|heat shock protein family D (Hsp60) member 1|60 kDa heat shock protein, mitochondrial|60 kDa chaperonin|CPN60|HSP-60|HSP-65|chaperonin 60|heat shock 60kD protein 1 (chaperonin)|heat shock 60kDa protein 1|heat shock protein 1 (chaperonin)|heat shock protein 60 (liver)|heat shock protein family D member 1|mitochondrial matrix protein P1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Hspd1, check out the Hspd1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Hspd1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-HSP60 Hspd1 Antibody (Monoclonal, LK1) (MA1049)
MA1049 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression and location of HSP60 and HSP10 in the heart tissue of heat-stressed rats
Hepatic mitochondrial and ER stress induced by defective PPAR? signaling in the pathogenesis of hepatic steatosis
Cui Yd, Du Yz, Lu Mx, Qiang Ck. J Insect Sci. 2010;10:100. Doi: 10.1673/031.010.10001. Cloning Of The Heat Shock Protein 60 Gene From The Stem Borer, Chilo Suppressalis, And Analysis Of Expression Characteristics Under Heat Stress.
Hager L, Li L, Pun H, Liu L, Hossain Ma, Maguire Gf, Naples M, Baker C, Magomedova L, Tam J, Adeli K, Cummins Cl, Connelly Pw, Ng Ds. J Biol Chem. 2012 Jun 8;287(24):20755-68. Doi: 10.1074/Jbc.M112.340919. Epub 2012 Apr 12. Lecithin:Cholesterol Ac...
Li L, Nan P, Zhai S, Wang L, Si S, Chang Z. Mol Cell Biochem. 2014 Feb;387(1-2):143-50. Doi: 10.1007/S11010-013-1879-0. Epub 2013 Nov 21. Molecular Cloning, Characterization, And Expression Of Hsp60 In Caudal Fin Regeneration Of Misgurnus Anguilli...
Li L, Zhai S, Wang L, Si S, Wu H, Chang Z. Fish Shellfish Immunol. 2014 Feb;36(2):401-8. Doi: 10.1016/J.Fsi.2013.12.016. Epub 2013 Dec 29. Hsp60 In Caudal Fin Regeneration From Paramisgurnus Dabryanus: Molecular Cloning And Expression Characteriza...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-HSP60 Hspd1 Antibody (Monoclonal, LK1)?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-HSP60 Hspd1 Antibody (Monoclonal, LK1)
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
