Anti-HSP60 Hspd1 Antibody (Monoclonal, LK1)

Product Name

Anti-HSP60 Hspd1 Antibody (Monoclonal, LK1)

View all HSP60 Antibodies

SKU/Catalog Number

MA1049

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HSP60 Hspd1 Antibody (Monoclonal, LK1) catalog # MA1049. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HSP60 Hspd1 Antibody (Monoclonal, LK1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1049)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

LK1

Isotype

Mouse IgG1

Immunogen

Recombinant human heat shock protein 60 (HSP60)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1049 is reactive to Hspd1 in Chicken, Human, Mouse, Rat

Applications

MA1049 is guaranteed for IHC, WB Boster Guarantee

Background of HSP60

Heat shock 60KD protein (HSP60) is a member of the chaperonin class of protein factors and the nuclear encoded mitochondrial HSP60 is required for the assembly into oligomeric complexes of proteins imported into the mitochondrial matrix. HSP60 is linked head to head comprising approximately 17 kb and consist of 12 exons. HSP60 is a self-molecule, it can downregulate adaptive immune responses by upregulating Tregs innately through TLR2 signaling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 2-4μg/ml, Human, chicken, rat
Immunohistochemistry (Paraffin-embedded Section), 4-8μg/ml, Human, chicken, mouse, rat, By Heat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of HSP60 using anti-HSP60 antibody (MA1049).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HSP60 antigen affinity purified monoclonal antibody (Catalog # MA1049) at 2 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for HSP60 at approximately 65 kDa. The expected band size for HSP60 is at 65 kDa.

Gene/Protein Information For Hspd1 (Source: Uniprot.org, NCBI)

Alternative Names

60 kDa chaperonin; Chaperonin 60; cpn60; GROEL; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1 (chaperonin); Heat shock protein 60; heat shock protein 65; HLD4; HSP60; HSP-60; HSP60SPG13; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; spastic paraplegia 13 (autosomal dominant); SPG13 Hspd1|Hsp60-30p, Hspd1|heat shock protein family D (Hsp60) member 1|60 kDa heat shock protein, mitochondrial|60 kDa chaperonin|CPN60|HSP-60|HSP-65|chaperonin 60|heat shock 60kD protein 1 (chaperonin)|heat shock 60kDa protein 1|heat shock protein 1 (chaperonin)|heat shock protein 60 (liver)|heat shock protein family D member 1|mitochondrial matrix protein P1

For more info on Hspd1, check out the Hspd1 Infographic

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In this infographic, you will see the following information for Hspd1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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