Click image to see more details
Product Info Summary
| SKU: | M01767-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Hu CD133 Purified PROM1 Monoclonal Antibody
SKU/Catalog Number
M01767-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD133 Purified PROM1 Monoclonal Antibody (Catalog# M01767-1). Tested in Flow Cytometry, WB, ICC application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD133 Purified PROM1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01767-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
293C3
Isotype
Mouse IgG2b
Immunogen
Human CD133 transfectants. The mouse monoclonal antibody 293C3 recognizes the extracellular epitope 2 on human CD133 (CD133/2), a 120 kDa glycoprotein of prominin family, expressed e.g. on progenitor cells. This antibody is important for identification of human renal progenitors.
Reactive Species
M01767-1 is reactive to PROM1 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
97.2 kDa
Background of PROM1
CD133 (prominin 1) is a 5-transmembrane glycoprotein with extracellular N- and intracellular C-terminus. CD133 function remains to be elucidated, but it can be used as a cancer stem cell marker. Its expression pattern in progenitor cells is similar to CD34, i.e. on hematopoietic stem cells in bone marrow, cord blood, neural stem cells, retinoblastoma, or endothelial precursor cells (not mature endothelial cells). It is being used for identification and isolation of hematopoietic stem cells, including isolation for stem cell transplantation. Expression of CD133 correlates with differentiation of human colon cancer cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01767-1 is guaranteed for Flow Cytometry, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry: Formaldehyde fixation can be used.
Flow cytometry: 1-4 µg/ml
Validation Images & Assay Conditions
Click image to see more details
Flow cytometry analysis (surface staining) of human peripheral whole blood showing CD133 positive stem cells (blue) and lymphocytes (red) stained using anti-CD133 (293C3) purified, GAM-FITC.
Specific Publications For Anti-Hu CD133 Purified PROM1 Monoclonal Antibody (M01767-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Hu CD133 Purified PROM1 Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Hu CD133 Purified PROM1 Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
