Anti-ONECUT2 Antibody Picoband™

Boster Bio Anti-ONECUT2 Antibody Picoband™ catalog # A09652-1. Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A09652-1
Size: 100μg/vial
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-ONECUT2 Antibody Picoband™

See all ONECUT2/OC-2 products

SKU/Catalog Number

A09652-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ONECUT2 Antibody Picoband™ catalog # A09652-1. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ONECUT2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09652-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence of human ONECUT2 (MKAAYTAYRCLTKDLEGCAMNPELTMESLGTLH).

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A09652-1 is reactive to ONECUT2 in Human, Mouse

Applications

A09652-1 is guaranteed for WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For ONECUT2 (Source: Uniprot.Org, NCBI)

Uniprot ID

O95948

Gene ID

9480

Gene Name

ONECUT2

Full Name

One cut domain family member 2

Weight

54.303kDa

Superfamily

CUT homeobox family

Alternative Names

one cut domain family member 2; HNF-6B; OC2; OC-2; one cut homeobox 2; ONECUT2 ONECUT2 OC-2, OC2 one cut homeobox 2 one cut domain family member 2|HNF-6-beta|ONECUT-2 homeodomain transcription factor|hepatocyte nuclear factor 6-beta|onecut 2|transcription factor ONECUT-2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ONECUT2, check out the ONECUT2 Infographic

ONECUT2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ONECUT2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Anti-ONECUT2 Antibody Picoband™

Question

See below the WB image, lot number and protocol we used for jejunal mucosa using anti-ONECUT2 antibody A09652-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-06

Question

Would A09652-1 anti-ONECUT2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

It shows on the product datasheet, A09652-1 anti-ONECUT2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-05

Question

We are currently using anti-ONECUT2 antibody A09652-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-15

Answer

The anti-ONECUT2 antibody (A09652-1) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-15

Question

Can you help my question with product A09652-1, anti-ONECUT2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

J. Patel

Verified customer

Asked: 2018-12-14

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09652-1 anti-ONECUT2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-12-14

Question

Does anti-ONECUT2 antibody A09652-1 work on pig WB with jejunal mucosa?

Verified Customer

Verified customer

Asked: 2018-02-22

Answer

Our lab technicians have not tested anti-ONECUT2 antibody A09652-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-ONECUT2 antibody A09652-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig jejunal mucosa in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-02-22

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for jejunal mucosa using anti-ONECUT2 antibody A09652-1. Let me know if you need anything else.

A. Yang

Verified customer

Asked: 2016-02-24

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-02-24

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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A09652-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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