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Product Info Summary
|Reactive Species:||Human, Mouse|
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Anti-ONECUT2 Antibody Picoband™
Boster Bio Anti-ONECUT2 Antibody Picoband™ catalog # A09652-1. Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ONECUT2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09652-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence of human ONECUT2 (MKAAYTAYRCLTKDLEGCAMNPELTMESLGTLH).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A09652-1 is reactive to ONECUT2 in Human, Mouse
A09652-1 is guaranteed for WB Boster Guarantee
Background of ONECUT2/OC-2
In molecular biology, the CUT domain (also known as ONECUT) is a DNA-binding motif which can bind independently or in cooperation with the homeodomain, which is often found downstream of the CUT domain. It is mapped to 18q21.31. This gene encodes a member of the onecut family of transcription factors, which are characterized by a cut domain and an atypical homeodomain. The protein binds to specific DNA sequences and stimulates expression of target genes, including genes involved in melanocyte and hepatocyte differentiation.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ONECUT2 using anti-ONECUT2 antibody (A09652-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ONECUT2 antigen affinity purified polyclonal antibody (Catalog # A09652-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ONECUT2 at approximately 54KD. The expected band size for ONECUT2 is at 54KD.
Gene/Protein Information For ONECUT2 (Source: Uniprot.org, NCBI)
One cut domain family member 2
CUT homeobox family
one cut domain family member 2; HNF-6B; OC2; OC-2; one cut homeobox 2; ONECUT2 ONECUT2 OC-2, OC2 one cut homeobox 2 one cut domain family member 2|HNF-6-beta|ONECUT-2 homeodomain transcription factor|hepatocyte nuclear factor 6-beta|onecut 2|transcription factor ONECUT-2*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on ONECUT2, check out the ONECUT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ONECUT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
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