Anti-Hu CD177 Purified Monoclonal Antibody

Product Name

Anti-Hu CD177 Purified Monoclonal Antibody

View all CD177 Antibodies

SKU/Catalog Number

M03958

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Hu CD177 Purified Monoclonal Antibody (Catalog# M03958). Tested in Flow Cytometry, IP, WB application(s). This antibody reacts with Primate, Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-Hu CD177 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03958)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

MEM-166

Isotype

Mouse IgG1

Immunogen

Human granulocytes. The antibody MEM-166 reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M03958 is reactive to CD177 in Human, Primate

Observed Molecular Weight

42 kDa

Calculated molecular weight

46.4 kDa

Background of CD177

CD177 (NB1/HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03958 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow cytometry: 1 μg/ml.
Western blotting: Non-reducing conditions.

Validation Images & Assay Conditions

See full target information CD177

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