Rat EPO/Erythropoietin PicoKine® Quick ELISA Kit

Erythropoietin/EPO ELISA kit, 120-minute protocol

Rat EPO/Erythropoietin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Epo in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Product Info Summary

SKU: FEK1351
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).
Assay Time: 120 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Rat EPO/Erythropoietin PicoKine® Quick ELISA Kit

See all Erythropoietin/EPO products

SKU/Catalog Number

FEK1351

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat EPO/Erythropoietin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Epo in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat EPO/Erythropoietin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1351)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: T44-K226

Sensitivity

<10 pg/ml

Assay Range

46.9 pg/ml - 3,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK1351 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1351 is reactive to EPO in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

FEK1351 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Erythropoietin/EPO

Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. This gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What’s more, EPO is also involved in the wound healing process.

Kit Components

Catalog number Description Quantity
FEK1351-CAP 96-well plate precoated with anti-Rat Erythropoietin/EPO antibody 1
EK1351-ST lyophilized recombinant Rat Erythropoietin/EPO standard 10 ng/tube
FEK1351-DA Biotinylated anti-Rat Erythropoietin/EPO detection antibody (50x) 100 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)046.993.8187.537575015003000
O.D.0.0000.0080.0160.0660.1620.4491.4202.290

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)10834213321133221373
Standard deviation5.425.3%970.596.6630.2686.49
CV (%)5%7.6%5.3%5.9%9.4%6.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Erythropoietin/EPO in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11081171101151123.643.2%
Sample 234237438637336816.264.4%
Sample 31332118912271391128480.666.2%
*number of samples for each test n=16.

Gene/Protein Information For EPO (Source: Uniprot.Org, NCBI)

Uniprot ID

P01588

Gene ID

2056

Gene Name

EPO

Full Name

Erythropoietin

Weight

21.307kDa

Superfamily

EPO/TPO family

Alternative Names

ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2 EPO DBAL, ECYT5, EP, MVCD2 erythropoietin erythropoietin|epoetin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on EPO, check out the EPO Infographic

EPO infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for EPO: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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