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Product Info Summary
| SKU: | M02158-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IHC-P, IHC-F |
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Product info
Product Name
Anti-Hu CD1b Purified Monoclonal Antibody
SKU/Catalog Number
M02158-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD1b Purified Monoclonal Antibody (Catalog# M02158-1). Tested in Flow Cytometry, IP, IHC-P, IHC-F application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD1b Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02158-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
SN13
Isotype
Mouse IgG1 kappa
Immunogen
A cell membrane antigen preparation that was isolated from normal human thymocytes. The mouse monoclonal antibody SN13 (also known as K5-1B8) recognizes an extracellular epitope of CD1b, a 44 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M02158-1 is reactive to CD1B in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
36.9 kDa
Background of CD1B
CD1b (also known as R1) together with CD1a and c, belongs to group 1 of CD1 antigens. These non-classical MHC-like glycoproteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. The trafficking routes of the particular CD1 types differ and correspond to their ability to bind and present different groups of antigens. Besides non-peptide glycolipid antigen presentation to CD1-restricted T cells, CD1b has been implicated in thymocyte development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02158-1 is guaranteed for Flow Cytometry, IP, IHC-P, IHC-F Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
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Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells using anti-human CD1b (SN13) purified antibody (concentration in sample 9 µg/ml, GAM PE) and anti-human CD14 (MEM-15) Pacific Blue™ antibody (4 µl reagent per milion cells in 100 µl of cell suspension).
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Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells using anti-human CD1b (SN13) purified antibody (concentration in sample 9 µg/ml, GAM PE) and anti-human HLA-DR (L243) APC antibody (10 µl reagent per milion cells in 100 µl of cell suspension).
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Separation of cells stained using anti-human CD1b (SN13) purified antibody (concentration in sample 9 µg/ml, GAM PE, red-filled) from cells unstained by primary antibody (GAM PE, black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells.
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Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD1b (SN13) purified antibody (concentration in sample 9 µg/ml, GAM PE).
Specific Publications For Anti-Hu CD1b Purified Monoclonal Antibody (M02158-1)
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