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Anti-Carboxypeptidase B2/CPB2 Antibody Picoband™
Boster Bio Anti-Carboxypeptidase B2/CPB2 Antibody Picoband™ catalog # PB9998. Tested in WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Carboxypeptidase B2/CPB2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9998)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived mouse CPB2 recombinant protein (Position: K165-D387). Mouse CPB2 shares 86.1% and 96.9% amino acid (aa) sequence identity with human and rat CPB2, respectively.
No cross reactivity with other proteins
PB9998 is reactive to Cpb2 in Mouse
PB9998 is guaranteed for WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CPB2 using anti-CPB2 antibody (PB9998).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPB2 antigen affinity purified polyclonal antibody (Catalog # PB9998) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPB2 at approximately 48 kDa. The expected band size for CPB2 is at 49 kDa.
Gene/Protein Information For Cpb2 (Source: Uniprot.Org, NCBI)
peptidase M14 family
carboxypeptidase B2 (plasma); carboxypeptidase B2 (plasma, carboxypeptidase U); Carboxypeptidase B2; Carboxypeptidase U; CPB2; CPU; CPUEC 126.96.36.199; EC 3.4.17; PCPB; Plasma carboxypeptidase B; TAFI; TAFIcarboxypeptidase B-like protein; Thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor Cpb2|1110032P04Rik, 4930405E17Rik, AI255929, C, CP, CPR, Cpu, TA, TAFI|carboxypeptidase B2 (plasma)|carboxypeptidase B2|carboxypeptidase R|carboxypeptidase U|thrombin-activable fibrinolysis inhibitor|thrombin-activatable fibrinolysis inhibitor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Cpb2, check out the Cpb2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cpb2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PB9998 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
INVOLVEMENT OF PROLYLCARBOXYPEPTIDASE IN THE EFFECT OF RUTAECARPINE ON THE REGRESSION OF MESENTERIC ARTERY HYPERTROPHY IN RENOVASCULAR HYPERTENSIVE RATS
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