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- Table of Contents
Facts about T-cell surface glycoprotein CD1b.
Human | |
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Gene Name: | CD1B |
Uniprot: | P29016 |
Entrez: | 910 |
Belongs to: |
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No superfamily |
CD1; CD1A; CD1b antigen; CD1B antigen, b polypeptide; CD1b molecule; CD1b; cortical thymocyte antigen CD1B; differentiation antigen CD1-alpha-3; MGC125990; MGC125991; R1; T-cell surface glycoprotein CD1b
Mass (kDA):
36.939 kDA
Human | |
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Location: | 1q23.1 |
Sequence: | 1; NC_000001.11 (158285406..158331531, complement) |
Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.
Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes.
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For internalization rates, Fabfragment assays have been used for the detection CD1b molecules at cell surfaces. The Fab-fragments could be detected on the cell's surface at 30 min, 60 min and 120 minutes after the additions of transferrin. Five independent experiments were conducted using monocyte-derived DCs from different donors. The graphs were derived from the data collected during the first measurement.
The CD1b/Ac2SGL-coated THP-1 cells were used to select the phages. The phage clones were then tested against CD1b/THP-1-unloaded cells. After four rounds and two rounds, eight phages clones showed preferential binding after being panned.
Cross-reactive T cells can be detected by immunogold labeling CD1b-expressing T cell cells. This method is useful because it gives a chemically defined target that can be used for future studies. SGLs or Sulfoglycolipids can only be found in Mycobacterium tuberculosis. The authors were capable of determining the molecular requirements for activation or binding of T-cell receptors and SGLs using computational simulations. The binding domains of CD1b showed near-complete collapse in the absence antigen, and spontaneously closed the binding domain entrance. These results indicate that CD1b is a preferred target for cross-reactive cells.
We used a murine anti CD1b mAb called BCD1b3.1 to detect CD1B. At RT, we blocked cells with 3% M–PBS. Next, we added recombinant CD1b from human with Ac2SGL. This antibody is expressed on DCs and macrophages as well as certain populations of B cell populations. This allows for detection of all DC communities.
There are significant implications for the development of molecules specific to Mtb infected cells. The identification of Mtb-infected cells is an important step towards developing new diagnostic and therapeutic approaches. Therefore, the development of new tools capable of discriminating Mtb-infected cells could provide new insights into the disease and aid in TB treatment. The ideal tools should be able to detect markers that are specific for Mtb-related infections, regardless of polymorphisms and genetic background.
Optical density reading against CD1b-Ac2SGL was used to establish a threshold for positivity in the immune system. A threshold of 3 was established. This is a higher level than the usual 2-to-1 ratio. CD1b–Ac2SGL may prove to be an important tool in diagnosing and predicting B-CLPDs. Why should we look into CD1d?
A single domain antibody dAbk11, recognized CD1b-Ac2SGL complexes. The purified dAbk11 antibody is immunoreactive to recombinant CD1b/Ac2SGL combinations. ELISAs were conducted using 9E10 mAb to detect the antibody. The results were presented with OD450 nm and standard deviation.
PMID: 2447586 by Martin L.H., et al. Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c.
PMID: 2701945 by Aruffo A., et al. Expression of cDNA clones encoding the thymocyte antigens CD1a, b, c demonstrates a hierarchy of exclusion in fibroblasts.
*More publications can be found for each product on its corresponding product page