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Product Info Summary
| SKU: | A06194-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-HuB/D ELAVL2 Antibody
SKU/Catalog Number
A06194-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-HuB/D ELAVL2 Antibody catalog # A06194-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HuB/D ELAVL2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06194-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ELAV2/4. AA range:61-110
Reactive Species
A06194-1 is reactive to ELAVL2 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
39.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06194-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
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Immunofluorescence analysis of HepG2 cells, using ELAV2/4 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of Hela cells using HuB/D Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from Jurkat and HUVEC cells, using ELAV2/4 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human spleen. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-HuB/D ELAVL2 Antibody (A06194-1)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-HuB/D ELAVL2 Antibody
Question
Here is the WB image, lot number and protocol we used for brain using anti-HuB/D antibody A06194-1. Please let me know if you require anything else.
M. Carter
Verified customer
Asked: 2017-10-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-10
Question
Is this A06194-1 anti-HuB/D antibody reactive to the isotypes of ELAVL2?
Verified Customer
Verified customer
Asked: 2017-08-01
Answer
The immunogen of A06194-1 anti-HuB/D antibody is Synthesized peptide derived from the Internal region of human HuB/D. at AA rangle: 30-110. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-08-01
Question
Will anti-HuB/D antibody A06194-1 work for WB with brain?
B. Carter
Verified customer
Asked: 2016-11-08
Answer
According to the expression profile of brain, ELAVL2 is highly expressed in brain. So, it is likely that anti-HuB/D antibody A06194-1 will work for WB with brain.
Boster Scientific Support
Answered: 2016-11-08
Question
I was wanting to use your anti-HuB/D antibody for WB for mouse brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain identification?
V. Rodriguez
Verified customer
Asked: 2016-08-01
Answer
It shows on the product datasheet, A06194-1 anti-HuB/D antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-08-01
