Anti-Angiostatin K1-3/PLG Antibody

Boster Bio Anti-Angiostatin K1-3/PLG Antibody catalog # RP1027. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: RP1027
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-Angiostatin K1-3/PLG Antibody

See all PLG products

SKU/Catalog Number

RP1027

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Angiostatin K1-3/PLG Antibody catalog # RP1027. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Angiostatin K1-3/PLG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1027)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Immunogen

E. coli-derived human Angiostatin K1-3 recombinant protein (Position: C103-C352).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1027 is reactive to PLG in Human

Applications

RP1027 is guaranteed for ELISA, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For PLG (Source: Uniprot.Org, NCBI)

Uniprot ID

P00747

Gene ID

5340

Gene Name

PLG

Full Name

Plasminogen

Weight

90.569kDa

Superfamily

peptidase S1 family

Alternative Names

DKFZp779M0222; EC 3.4.21; EC 3.4.21.7; plasmin; Plasminogen; Plg PLG plasminogen plasminogen|plasmin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PLG, check out the PLG Infographic

PLG infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PLG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for RP1027

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17 Customer Q&As for Anti-Angiostatin K1-3/PLG Antibody

Question

We are currently using anti-Angiostatin K1-3/PLG antibody RP1027 for human tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?

S. Carter

Verified customer

Asked: 2019-12-09

Answer

The anti-Angiostatin K1-3/PLG antibody (RP1027) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-09

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right lobe of liver using anti-Angiostatin K1-3/PLG antibody RP1027. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-19

Question

We are interested in using your anti-Angiostatin K1-3/PLG antibody for activation of matrix metalloproteinases studies. Has this antibody been tested with western blotting on hepg2 whole cell lysates? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-11-18

Answer

Thank you for your inquiry. This RP1027 anti-Angiostatin K1-3/PLG antibody is validated on hepg2 whole cell lysates. It is guaranteed to work for ELISA, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-11-18

Question

Does anti-Angiostatin K1-3/PLG antibody RP1027 work on pig ELISA with liver?

S. Parker

Verified customer

Asked: 2019-08-29

Answer

Our lab technicians have not validated anti-Angiostatin K1-3/PLG antibody RP1027 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Angiostatin K1-3/PLG antibody RP1027 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in ELISA, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-29

Question

I was wanting to use your anti-Angiostatin K1-3/PLG antibody for WB for human right lobe of liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human right lobe of liver identification?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

As indicated on the product datasheet, RP1027 anti-Angiostatin K1-3/PLG antibody has been validated for ELISA, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human right lobe of liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-09

Question

Will anti-Angiostatin K1-3/PLG antibody RP1027 work for WB with right lobe of liver?

W. Singh

Verified customer

Asked: 2019-07-04

Answer

According to the expression profile of right lobe of liver, PLG is highly expressed in right lobe of liver. So, it is likely that anti-Angiostatin K1-3/PLG antibody RP1027 will work for WB with right lobe of liver.

Boster Scientific Support

Answered: 2019-07-04

Question

I see that the anti-Angiostatin K1-3/PLG antibody RP1027 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-04-02

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-04-02

Question

We bought anti-Angiostatin K1-3/PLG antibody for ELISA on right lobe of liver in a previous project. I am using human, and We want to use the antibody for IHC next. I would like examining right lobe of liver as well as milk in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2018-09-18

Answer

I have checked the website and datasheets of our anti-Angiostatin K1-3/PLG antibody and it seems that RP1027 has been validated on human in both ELISA and IHC. Thus RP1027 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-09-18

Question

Is there a BSA free version of anti-Angiostatin K1-3/PLG antibody RP1027 available?

Verified Customer

Verified customer

Asked: 2017-12-26

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Angiostatin K1-3/PLG antibody RP1027 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-12-26

Question

Is this RP1027 anti-Angiostatin K1-3/PLG antibody reactive to the isotypes of PLG?

Verified Customer

Verified customer

Asked: 2017-12-11

Answer

The immunogen of RP1027 anti-Angiostatin K1-3/PLG antibody is E. coli-derived human Angiostatin K1-3 recombinant protein(Position: C103-C352). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-12-11

Question

We were content with the WB result of your anti-Angiostatin K1-3/PLG antibody. However we have seen positive staining in kidney secreted using this antibody. Is that expected? Could you tell me where is PLG supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-07-06

Answer

Based on literature, kidney does express PLG. Generally PLG expresses in secreted. Regarding which tissues have PLG expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 14574404, 24275569
Milk, Pubmed ID: 18780401

Boster Scientific Support

Answered: 2017-07-06

Question

you antibody to test anti-Angiostatin K1-3/PLG antibody RP1027 on human right lobe of liver for research purposes, then I may be interested in using anti-Angiostatin K1-3/PLG antibody RP1027 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

L. Jones

Verified customer

Asked: 2017-01-09

Answer

The products we sell, including anti-Angiostatin K1-3/PLG antibody RP1027, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-01-09

Question

Is a blocking peptide available for product anti-Angiostatin K1-3/PLG antibody (RP1027)?

C. Mitchell

Verified customer

Asked: 2015-05-14

Answer

We do provide the blocking peptide for product anti-Angiostatin K1-3/PLG antibody (RP1027). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2015-05-14

Question

We have observed staining in human liver. What should we do? Is anti-Angiostatin K1-3/PLG antibody supposed to stain liver positively?

M. Williams

Verified customer

Asked: 2015-04-06

Answer

From literature liver does express PLG. From Uniprot.org, PLG is expressed in right lobe of liver, liver, kidney, milk, among other tissues. Regarding which tissues have PLG expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 14574404, 24275569
Milk, Pubmed ID: 18780401

Boster Scientific Support

Answered: 2015-04-06

Question

See below the WB image, lot number and protocol we used for right lobe of liver using anti-Angiostatin K1-3/PLG antibody RP1027. Please let me know if you require anything else.

P. Zhang

Verified customer

Asked: 2014-10-15

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-10-15

Question

I have a question about product RP1027, anti-Angiostatin K1-3/PLG antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

E. Yang

Verified customer

Asked: 2014-09-02

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1027 anti-Angiostatin K1-3/PLG antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-09-02

Question

Does RP1027 anti-Angiostatin K1-3/PLG antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

P. Rodriguez

Verified customer

Asked: 2013-11-21

Answer

As indicated on the product datasheet, RP1027 anti-Angiostatin K1-3/PLG antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-11-21

Conjugation

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RP1027
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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