Human PLAT/TPA ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human PLAT/TPA ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human PLAT in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EZ0897
Size: For 5 plates, 96 wells each
Reactive Species: Human
Application: ELISA

Product Name

Human PLAT/TPA ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all t-Plasminogen Activator/tPA products

SKU/Catalog Number

EZ0897

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Human PLAT/TPA ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human PLAT in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human PLAT/TPA ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0897)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: M1-P562

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0897 is reactive to PLAT in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Application Guarantee

EZ0897 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ0897-CA Rabbit anti- human PLAT polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ0897-DA Biotinylated goat anti- human PLAT polyclonal antibody (Detection Antibody) 500 µl, 3 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ0897-ST Lyophilized recombinant human PLAT standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0290.1060.1930.3370.6141.0661.7512.326

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)1915129252447321216
Standard deviation11.6524.15017.5742.581.5
CV (%)6.1%4.7%5.4%7.2%5.8%6.7%

Gene/Protein Information For PLAT (Source: Uniprot.Org, NCBI)

Uniprot ID

P00750

Gene ID

5327

Gene Name

PLAT

Full Name

Tissue-type plasminogen activator

Weight

62.917kDa

Superfamily

peptidase S1 family

Alternative Names

Alteplase; DKFZp686I03148; EC 3.4.21; EC 3.4.21.68; plasminogen activator, tissue type; plasminogen activator, tissue; PLAT; Reteplase; tissue plasminogen activator (t-PA); tissue-type plasminogen activator; TPA; T-PA; tPlasminogen Activator; t-Plasminogen Activator PLAT T-PA, TPA plasminogen activator, tissue type tissue-type plasminogen activator|alteplase|plasminogen/activator kringle|reteplase|t-plasminogen activator

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PLAT, check out the PLAT Infographic

PLAT infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PLAT: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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