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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.|
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Human PLAT/TPA ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human PLAT/TPA ELISA Kit PicoKine™ (96 Tests). Quantitate Human PLAT in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human PLAT/TPA ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0897)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: CHO; Immunogen sequence: M1-P562
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0897 is reactive to PLAT in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
EK0897 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0897-CAP||Anti-Human PLAT Pre-coated 96-well strip microplate||1|
|EK0897-ST||Human PLAT Standard||2 vials, 10 ng/tube|
|EK0897-DA||Human PLAT Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human PLAT/TPA PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect t-Plasminogen Activator/tPA, Dilution ratio of 1:20, concentration in serum is around 30ng/ml.
Some articles we found to cite concentrations of t-Plasminogen Activator/tPA in samples: 17532723 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human PLAT/TPA ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of t-Plasminogen Activator/tPA in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For PLAT (Source: Uniprot.Org, NCBI)
Tissue-type plasminogen activator
peptidase S1 family
Alteplase; DKFZp686I03148; EC 3.4.21; EC 126.96.36.199; plasminogen activator, tissue type; plasminogen activator, tissue; PLAT; Reteplase; tissue plasminogen activator (t-PA); tissue-type plasminogen activator; TPA; T-PA; tPlasminogen Activator; t-Plasminogen Activator PLAT T-PA, TPA plasminogen activator, tissue type tissue-type plasminogen activator|alteplase|plasminogen/activator kringle|reteplase|t-plasminogen activator*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on PLAT, check out the PLAT Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PLAT: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0897 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Hu, M., Chang, H., Zhang, H., Wang, J., Lei, W.X., Li, B.C.,…, & Ji, J. (2017). Mechanical Adaptability of the MMP-Responsive Film Improves the Functionality of Endothelial Cell Monolayer. Advanced Healthcare Materials. Advance online publication....
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