Overview
| Product Name |
Anti-EPO/Erythropoietin Antibody
See more Erythropoietin/EPO products |
| Catalog# |
RP1004 |
| Pack Size |
100μg/vial |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-EPO/Erythropoietin Antibody catalog # RP1004. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. |
| Cite This Product |
Anti-EPO/Erythropoietin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1004)
|
| Antibodies Validation |
Antibodies Validation Information
|
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Anti-EPO/Erythropoietin Antibody may replace the following items: sc 1310|sc 7956|sc 5290|sc 80995|sc 1310 R. |
Product Specs
| Host |
Rabbit |
| Reactive Species |
Human |
| Applications |
ELISA, IHC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $100. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
E. coli-derived human EPO recombinant protein (Position: A1-R166). |
| Cross Reactivity |
No cross reactivity with other proteins |
Gene/Protein Basic Information For EPO (Source: Uniprot.org, NCBI)
| Uniprot Id | P01588 |
|---|
| NCBI Gene Id | 2056 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | EPO |
|---|
| Protein Name | Erythropoietin |
|---|
| Superfamily | EPO/TPO family |
|---|
| Alternative Names | Erythropoietin/EPO|ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2 |
|---|
| Molecular Weight | 21307 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on EPO, check out the EPO Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for EPO: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the EPO infographic
Our Boster Quality Guarantee for Anti-EPO/Erythropoietin Antibody covers its use in the following applications.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Anti-human EPO antibody, RP1004, Western blotting
Lane 1: Recombinant human EPO Protein 10ng
Lane 2: Recombinant human EPO Protein 5ng
Lane 3: Recombinant human EPO Protein 2
Anti-human EPO antibody, RP1004, IHC(P)
IHC(P): Human Placenta Tissue
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Did You Know ?
That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
Other Recommended Resources
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Questions and answers from customers related to RP1004 Anti-EPO/Erythropoietin Antibody
5 Related Questions
Question
We were satisfied with the WB result of your anti-EPO/Erythropoietin antibody. However we have been able to see positive staining in brain secreted. using this antibody. Is that expected? Could you tell me where is EPO supposed to be expressed?
K. Moore
Asked: 2020-04-02
Answer
Based on literature, brain does express EPO. Generally EPO expresses in secreted. Regarding which tissues have EPO expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Urine, Pubmed ID: 3949763, 6698989, 3346214, 3219367
Boster Scientific Support
Answered: 2020-04-02
Question
We are currently using anti-EPO/Erythropoietin antibody RP1004 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Asked: 2019-09-19
Answer
The anti-EPO/Erythropoietin antibody (RP1004) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-19
Question
We have been able to see staining in human urine. Are there any suggestions? Is anti-EPO/Erythropoietin antibody supposed to stain urine positively?
Verified Customer
Asked: 2018-10-23
Answer
From what I have seen in literature urine does express EPO. From what I have seen in Uniprot.org, EPO is expressed in right lobe of liver, brain, urine, among other tissues. Regarding which tissues have EPO expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Urine, Pubmed ID: 3949763, 6698989, 3346214, 3219367
Boster Scientific Support
Answered: 2018-10-23
Question
you antibody using your anti-EPO/Erythropoietin antibody for erythropoietin activates ras studies. Has this antibody been tested with western blotting on human placenta tissue? We would like to see some validation images before ordering.
Verified Customer
Asked: 2018-07-06
Answer
We appreciate your inquiry. This RP1004 anti-EPO/Erythropoietin antibody is tested on human placenta tissue. It is guaranteed to work for ELISA, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-07-06
Question
Our lab used your anti-EPO/Erythropoietin antibody for IHC on brain in a previous experiment. I am using human, and I plan to use the antibody for WB next. I would like examining brain as well as right lobe of liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Asked: 2017-10-27
Answer
I have checked the website and datasheets of our anti-EPO/Erythropoietin antibody and it appears that RP1004 has been validated on human in both IHC and WB. Thus RP1004 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-10-27
