Anti-EPO/Erythropoietin Antibody

Boster Bio Anti-EPO/Erythropoietin Antibody catalog # RP1004. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: RP1004
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-EPO/Erythropoietin Antibody

See all Erythropoietin/EPO products

SKU/Catalog Number

RP1004

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-EPO/Erythropoietin Antibody catalog # RP1004. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EPO/Erythropoietin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1004)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Immunogen

E. coli-derived human EPO recombinant protein (Position: A1-R166).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1004 is reactive to EPO in Human

Applications

RP1004 is guaranteed for ELISA, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For EPO (Source: Uniprot.Org, NCBI)

Uniprot ID

P01588

Gene ID

2056

Gene Name

EPO

Full Name

Erythropoietin

Weight

21.307kDa

Superfamily

EPO/TPO family

Alternative Names

ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2 EPO DBAL, ECYT5, EP, MVCD2 erythropoietin erythropoietin|epoetin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on EPO, check out the EPO Infographic

EPO infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for EPO: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for RP1004

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5 Customer Q&As for Anti-EPO/Erythropoietin Antibody

Question

We were satisfied with the WB result of your anti-EPO/Erythropoietin antibody. However we have been able to see positive staining in brain secreted. using this antibody. Is that expected? Could you tell me where is EPO supposed to be expressed?

K. Moore

Verified customer

Asked: 2020-04-02

Answer

Based on literature, brain does express EPO. Generally EPO expresses in secreted. Regarding which tissues have EPO expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Urine, Pubmed ID: 3949763, 6698989, 3346214, 3219367

Boster Scientific Support

Answered: 2020-04-02

Question

We are currently using anti-EPO/Erythropoietin antibody RP1004 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

The anti-EPO/Erythropoietin antibody (RP1004) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-19

Question

We have been able to see staining in human urine. Are there any suggestions? Is anti-EPO/Erythropoietin antibody supposed to stain urine positively?

Verified Customer

Verified customer

Asked: 2018-10-23

Answer

From what I have seen in literature urine does express EPO. From what I have seen in Uniprot.org, EPO is expressed in right lobe of liver, brain, urine, among other tissues. Regarding which tissues have EPO expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Urine, Pubmed ID: 3949763, 6698989, 3346214, 3219367

Boster Scientific Support

Answered: 2018-10-23

Question

you antibody using your anti-EPO/Erythropoietin antibody for erythropoietin activates ras studies. Has this antibody been tested with western blotting on human placenta tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-07-06

Answer

We appreciate your inquiry. This RP1004 anti-EPO/Erythropoietin antibody is tested on human placenta tissue. It is guaranteed to work for ELISA, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-07-06

Question

Our lab used your anti-EPO/Erythropoietin antibody for IHC on brain in a previous experiment. I am using human, and I plan to use the antibody for WB next. I would like examining brain as well as right lobe of liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2017-10-27

Answer

I have checked the website and datasheets of our anti-EPO/Erythropoietin antibody and it appears that RP1004 has been validated on human in both IHC and WB. Thus RP1004 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-10-27

Conjugation

Size

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RP1004
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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