Overview
| Product Name |
Anti-EPO/Erythropoietin Antibody
See all Erythropoietin/EPO primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
RP1004 |
| Pack Size |
100μg/vial |
| Storage & Handling |
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing. |
| Description |
Polyclonal antibody for Erythropoietin/EPO detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. Erythropoietin/EPO information: Molecular Weight: 21307 MW; Subcellular Localization: Secreted; Tissue Specificity: Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. |
| Cite This Product |
Anti-EPO/Erythropoietin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1004)
|
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Anti-EPO/Erythropoietin Antibody may replace the following items: sc 1310|sc 7956|sc 5290|sc 80995|sc 1310 R. |
Product Specs
| Host |
Rabbit |
| Reactivity/Species |
Human |
| Applications |
ELISA, IHC, WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Related Products |
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round. |
| Immunogen |
E. coli-derived human EPO recombinant protein(Position: A1-R166). |
| Cross Reactivity |
No cross reactivity with other proteins |
Gene/Protein Basic Information For EPO (Source: Uniprot.org, NCBI)
| Uniprot Id | P01588 |
|---|
| NCBI Gene Id | 2056 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | EPO |
|---|
| Protein Name | Erythropoietin |
|---|
| Superfamily | EPO/TPO family |
|---|
| Alternative Names | Erythropoietin/EPO|ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2 |
|---|
| Molecular Weight | 21307 |
|---|
| Post Tranlational Modifications | Phosphorylation; Glycosylation; Carbamylation; Oxidation; Cleavage; Dephosphorylation; Biotinylation; Hydroxylation; Reduction; Ubiquitination; Nitration; Methylation; Acetylation; Deglycosylation; Iodination; Bromination; Demethylation; Ribosylation; Geranylgeranylation; Phosphorothioation |
|---|
| Subcellular Localization | Secreted. |
|---|
| Gene Location | 7q22.1, on Chromasome 7, gene sequence: NC_000007.14 (100720468..100723700) |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For EPO (Source: Uniprot.org, NCBI)
| Protein Function | Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3. |
|---|
| Tissue Specificity | Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. |
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| Interacting Proteins | EPHB4; EPOR |
|---|
| Related Diseases | Anemia; Kidney Failure, Chronic; Hypoxia; Neoplasms; Kidney Diseases; Malignant Neoplasms; Kidney Failure; Inflammation; Anoxia; Ischemia; Leukemia; Polycythemia; Hemorrhage; Hypertensive Disease; Iron Deficiency; Tumor Angiogenesis; Acute Erythroblastic Leukemia; Infarction; Erythrocytosis; Dysmyelopoietic Syndromes |
|---|
| Related Pathways | Secretion; Cell Proliferation; Pathogenesis; Angiogenesis; Neuroprotection; Cell Death; Cell Growth; Response To Hypoxia; Transport; Response To Erythropoietin; Regeneration; Cell Cycle; Localization; Cell Differentiation; Glomerular Filtration; Glycosylation; Excretion; Immune Response; Inflammatory Response; Neurogenesis |
|---|
| Background | EPO, Erythropoietin, is an acidic glycoprotein hormone with a molecular mass of 34 kD. It is a cytokine for erythrocyte(red blood cell) precursors in the bone marrow. This gene is mapped to 7q22.1. It is produced by interstitial fibroblasts in the kidney in close association with peritubular capillary and tubular epithelial cells. It is also produced in perisinusoidal cells in the liver. While liver production predominates in the fetal and perinatal period, renal production is predominant during adulthood. As the prime regulator of red cell production, its major functions are to promote erythroid differentiation and to initiate hemoglobin synthesis. It also has other known biological functions. For example, erythropoietin plays an important role in the brain's response to neuronal injury. EPO is also involved in the wound healing process. |
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| Scientific References | PMID: 3838366 by Jacobs K., et al. Isolation and characterization of genomic and cDNA clones of human erythropoietin.
PMID: 3865178 by Lin F.-K., et al. Cloning and expression of the human erythropoietin gene. |
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Our Boster Quality Guarantee for Anti-EPO/Erythropoietin Antibody covers its use in the following applications.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Anti-human EPO antibody, RP1004, Western blotting
Lane 1: Recombinant human EPO Protein 10ng
Lane 2: Recombinant human EPO Protein 5ng
Lane 3: Recombinant human EPO Protein 2
Anti-human EPO antibody, RP1004, IHC(P)
IHC(P): Human Placenta Tissue
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all Erythropoietin/EPO primary antibodies,
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