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Product Info Summary
| SKU: | RP1004 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-EPO/Erythropoietin Antibody Picoband®
SKU/Catalog Number
RP1004
BA14163 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-EPO/Erythropoietin Antibody catalog # RP1004. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-EPO/Erythropoietin Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1004)
Host
Rabbit
Contents
Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human EPO recombinant protein (Position: M1-R166).
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1004 is reactive to EPO in Human
Observed Molecular Weight
26 kDa
Calculated molecular weight
21.3 kDa
Background of EPO
EPO, Erythropoietin, is an acidic glycoprotein hormone with a molecular mass of 34 kD. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. This gene is mapped to 7q22.1. It is produced by interstitial fibroblasts in the kidney in close association with peritubular capillary and tubular epithelial cells. It is also produced in perisinusoidal cells in the liver. While liver production predominates in the fetal and perinatal period, renal production is predominant during adulthood. As the prime regulator of red cell production, its major functions are to promote erythroid differentiation and to initiate hemoglobin synthesis. It also has other known biological functions. For example, erythropoietin plays an important role in the brain's response to neuronal injury. EPO is also involved in the wound healing process.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1004 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Human
Positive Control
IHC: Human Placenta Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-human EPO antibody, RP1004, Western blotting
Lane 1: Recombinant human EPO Protein 10ng
Lane 2: Recombinant human EPO Protein 5ng
Lane 3: Recombinant human EPO Protein 2
Click image to see more details
Anti-human EPO antibody, RP1004, IHC(P)
IHC(P): Human Placenta Tissue
Specific Publications For Anti-EPO/Erythropoietin Antibody Picoband® (RP1004)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-EPO/Erythropoietin Antibody Picoband®
Question
We were satisfied with the WB result of your anti-EPO/Erythropoietin antibody. However we have been able to see positive staining in brain secreted. using this antibody. Is that expected? Could you tell me where is EPO supposed to be expressed?
K. Moore
Verified customer
Asked: 2020-04-02
Answer
Based on literature, brain does express EPO. Generally EPO expresses in secreted. Regarding which tissues have EPO expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Urine, Pubmed ID: 3949763, 6698989, 3346214, 3219367
Boster Scientific Support
Answered: 2020-04-02
Question
We are currently using anti-EPO/Erythropoietin antibody RP1004 for human tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
The anti-EPO/Erythropoietin antibody (RP1004) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-19
Question
We have been able to see staining in human urine. Are there any suggestions? Is anti-EPO/Erythropoietin antibody supposed to stain urine positively?
Verified Customer
Verified customer
Asked: 2018-10-23
Answer
From what I have seen in literature urine does express EPO. From what I have seen in Uniprot.org, EPO is expressed in right lobe of liver, brain, urine, among other tissues. Regarding which tissues have EPO expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Urine, Pubmed ID: 3949763, 6698989, 3346214, 3219367
Boster Scientific Support
Answered: 2018-10-23
Question
you antibody using your anti-EPO/Erythropoietin antibody for erythropoietin activates ras studies. Has this antibody been tested with western blotting on human placenta tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-07-06
Answer
We appreciate your inquiry. This RP1004 anti-EPO/Erythropoietin antibody is tested on human placenta tissue. It is guaranteed to work for ELISA, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-07-06
Question
Our lab used your anti-EPO/Erythropoietin antibody for IHC on brain in a previous experiment. I am using human, and I plan to use the antibody for WB next. I would like examining brain as well as right lobe of liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-10-27
Answer
I have checked the website and datasheets of our anti-EPO/Erythropoietin antibody and it appears that RP1004 has been validated on human in both IHC and WB. Thus RP1004 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-10-27
