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Pack Size:100μg/vial
Validated Species:Human
Application:ELISA, IHC, WB
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Product Name Anti-human FGF2 Antibody
SKU/Catalog Number RP1006
Description Rabbit IgG polyclonal antibody for Fibroblast growth factor 2(FGF2) detection. Tested with WB, IHC-P, ELISA in Human.
Cite This Product Anti-human FGF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1006)
Replacement Item This antibody may replace the following items: sc-1360|sc-136255|sc-1390|sc-1390-R|sc-16811|sc-1884|sc-27173|sc-271847|sc-292583|sc-365106|sc-50293|sc-74412|sc-74413|sc-79|sc-79-G|sc-7911 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application ELISA, IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen E.coli-derived human FGF2 recombinant protein(Position: P143-S288).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial


Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source:

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name FGF2
Protein Name Fibroblast growth factor 2
Molecular Weight 30770 MW
Protein Function Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. .
Tissue Specificity Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. .
Sequence Similarities Belongs to the heparin-binding growth factors family.
Subcellular Localization Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
Uniprot ID P09038
Alternative Names Fibroblast growth factor 2;FGF-2;Basic fibroblast growth factor;bFGF;Heparin-binding growth factor 2;HBGF-2;FGF2;FGFB;
Research Areas |cardiovascular|angiogenesis|growth factors|fgf| signal transduction|growth factors/hormones| neuroscience|neurology process|neurogenesis| stem cells|neural stem cells|extracellular| cancer|invasion/microenvironment|angiogenic growth factors| developmental biology|organogenesis|excretory system development|kidney development|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Fibroblast growth factor 2

FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor(FGF) family. Fibroblast growth factors(FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.

Anti-human FGF2 Antibody Images

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Anti-human FGF2 Antibody
Anti- FGF2 antibody, RP1006, Western blotting
All lanes: Anti FGF2(RP1006) at 0.5ug/ml
Lane 1: Recombinant Human FGF2 Protein 10ng
Lane 2: Recombinant Human FGF2 Protein 5ng
Lane 3: Recombinant Human FGF2 Protein 2.5ng
Predicted bind size: 17KD
Observed bind size: 17KD
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Zhou B, Ma R, Si W, Li S, Xu Y, Tu X, Wang Q. Cancer Lett. 2013 Jun 10;333(2):159-69. Doi: 10.1016/J.Canlet.2013.01.028. Epub 2013 Jan 22. Microrna-503 Targets Fgf2 And Vegfa And Inhibits Tumor Angiogenesis And Growth.
Hu C, Zhang T, Ren B, Deng Z, Cai L, Lei J, Ping A. Med Sci Monit. 2015 Apr 27;21:1200-6. Doi: 10.12659/Msm.892939. Effect Of Vacuum-Assisted Closure Combined With Open Bone Grafting To Promote Rabbit Bone Graft Vascularization.
Wang J, Wang Z, Yao Y, Wu J, Tang X, Gu T, Li G. Tuberculosis (Edinb). 2015 Jul;95(4):505-14. Doi: 10.1016/J.Tube.2015.04.006. Epub 2015 May 9. The Fibroblast Growth Factor-2 Arrests Mycobacterium Avium Sp. Paratuberculosis Growth And Immunomodula...

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.