Anti-Fibroblast growth factor 2 FGF2 Antibody
Rabbit IgG polyclonal antibody for Fibroblast growth factor 2(FGF2) detection. Tested with WB, IHC-P, ELISA in Human. Cited in 6 publication(s).
Anti-Fibroblast growth factor 2 FGF2 Antibody Info At A Glance
|Applications:||ELISA, IHC, WB|
|Product Name||Anti-Fibroblast growth factor 2 FGF2 Antibody
See all FGF basic/FGF2/bFGF primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for FGF basic/FGF2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. FGF basic/FGF2 information: Molecular Weight: 30770 MW; Subcellular Localization: Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57; Tissue Specificity: Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue.|
|Cite This Product||Anti-Fibroblast growth factor 2 FGF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1006)|
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|Anti-Fibroblast growth factor 2 FGF2 Antibody may replace the following items: sc 1360|sc 136255|sc 1390|sc 1390 R|sc 16811|sc 1884|sc 27173|sc 271847|sc 292583|sc 365106|sc 50293|sc 74412|sc 74413|sc 79|sc 79 G|sc 7911.|
|Applications||ELISA, IHC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||E.coli-derived human FGF2 recombinant protein(Position: P143-S288).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For FGF2 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||2247|
|Species Of This Entry||Human|
|Protein Name||Fibroblast growth factor 2|
|Superfamily||heparin-binding growth factors family|
|Alternative Names||FGF basic/FGF2/bFGF|basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Glycosylation; Dephosphorylation; Methylation; Acetylation; Biotinylation; Reduction; Oxidation; Sulphation; Gpi Anchoring; Ubiquitination; Iodination; Farnesylation; Demethylation; Phosphorothioation; Hydroxylation; Deacetylation; Palmitoylation; Deglycosylation|
|Subcellular Localization||Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.|
|Gene Location||4q28.1, on Chromasome 4, gene sequence: NC_000004.12 (122826708..122898236)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For FGF2 (Source: Uniprot.org, NCBI)
|Protein Function||Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:8663044, PubMed:28302677). Functions as a potent mitogen in vitro (PubMed:3732516, PubMed:3964259). Can induce angiogenesis (PubMed:23469107, PubMed:28302677).|
|Tissue Specificity||Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue.|
|Interacting Proteins||CASP1; FGFBP1; FGFR1; FGFR2; PTX3|
|Related Diseases||Tumor Angiogenesis; Neoplasms; Malignant Neoplasms; Pathologic Neovascularization; Tissue Adhesions; Carcinoma; Nervousness; Malignant Paraganglionic Neoplasm; Hyperplasia; Mammary Neoplasms; Craniosynostosis; Inflammation; Neoplasm Metastasis; Hypoxia; Hypertrophy; Malignant Neoplasm Of Breast; Leukemia; Ischemia; Cell Transformation, Neoplastic|
|Related Pathways||Angiogenesis; Cell Proliferation; Regeneration; Cell Growth; Secretion; Cell Cycle; Neurogenesis; Cell Death; Localization; Cell Differentiation; Cell Migration; Wound Healing; Cell Adhesion; Pathogenesis; Endothelial Cell Proliferation; Tube Formation; Osteoblast Differentiation; Ossification; Sulfation; Brain Development|
|Background||FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor(FGF) family. Fibroblast growth factors(FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.|
|Scientific References||PMID: 3472745 by Abraham J.A., et al. Human basic fibroblast growth factor: nucleotide sequence, genomic organization, and expression in mammalian cells.|
PMID: 3780670 by Abraham J.A., et al. Human basic fibroblast growth factor: nucleotide sequence and genomic organization.
Our Boster Quality Guarantee for Anti-Fibroblast growth factor 2 FGF2 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti FGF2(RP1006) at 0.5ug/ml
Lane 1: Recombinant Human FGF2 Protein 10ng
Lane 2: Recombinant Human FGF2 Protein 5ng
Lane 3: Recombinant Human FGF2 Protein 2.5ng
Predicted bind size: 17KD
Observed bind size: 17KD
FGF2 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FGF2 Antibody (RP1006) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 6
|Wang J, Wang Z, Yao Y, Wu J, Tang X, Gu T, Li G. Tuberculosis (Edinb). 2015 Jul;95(4):505-14. Doi: 10.1016/J.Tube.2015.04.006. Epub 2015 May 9. The Fibroblast Growth Factor-2 Arrests Mycobacterium Avium Sp. Paratuberculosis Growth And Immunomodula...||PubMed ID 25999285|
|Hu C, Zhang T, Ren B, Deng Z, Cai L, Lei J, Ping A. Med Sci Monit. 2015 Apr 27;21:1200-6. Doi: 10.12659/Msm.892939. Effect Of Vacuum-Assisted Closure Combined With Open Bone Grafting To Promote Rabbit Bone Graft Vascularization.||PubMed ID 25913359|
|Zhou B, Ma R, Si W, Li S, Xu Y, Tu X, Wang Q. Cancer Lett. 2013 Jun 10;333(2):159-69. Doi: 10.1016/J.Canlet.2013.01.028. Epub 2013 Jan 22. Microrna-503 Targets Fgf2 And Vegfa And Inhibits Tumor Angiogenesis And Growth.||PubMed ID 23352645|
|Advanced glycation end-products stimulate basic fibroblast growth factor expression in cultured M?ller cells||PubMed ID 23129015|
|Astragalosides promote angiogenesis via vascular endothelial growth factor and basic fibroblast growth factor in a rat model of myocardial infarction||PubMed ID 26352430|
|Effect of CPU-XT-008, a combretastatin A-4 analogue, on the proliferation, apoptosis and expression of vascular endothelial growth factor and basic fibroblast growth factor in human umbilical vein endothelial cells||PubMed ID 26870239|
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Note: sample size takes longer to process, average 5-7 days.
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