|Application:||ELISA, IHC, WB|
Data & Images
|Product Name||Anti-human FGF2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Fibroblast growth factor 2(FGF2) detection. Tested with WB, IHC-P, ELISA in Human.|
|Cite This Product||Anti-human FGF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1006)|
|Replacement Item||This antibody may replace the following items: sc-1360|sc-136255|sc-1390|sc-1390-R|sc-16811|sc-1884|sc-27173|sc-271847|sc-292583|sc-365106|sc-50293|sc-74412|sc-74413|sc-79|sc-79-G|sc-7911 from Santa Cruz Biotechnology.|
|Application||ELISA, IHC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||E.coli-derived human FGF2 recombinant protein(Position: P143-S288).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Fibroblast growth factor 2|
|Molecular Weight||30770 MW|
|Protein Function||Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. .|
|Tissue Specificity||Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue. .|
|Sequence Similarities||Belongs to the heparin-binding growth factors family.|
|Subcellular Localization||Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.|
|Alternative Names||Fibroblast growth factor 2;FGF-2;Basic fibroblast growth factor;bFGF;Heparin-binding growth factor 2;HBGF-2;FGF2;FGFB;|
|Research Areas|||cardiovascular|angiogenesis|growth factors|fgf| signal transduction|growth factors/hormones| neuroscience|neurology process|neurogenesis| stem cells|neural stem cells|extracellular| cancer|invasion/microenvironment|angiogenic growth factors| developmental biology|organogenesis|excretory system development|kidney development||
Background for Fibroblast growth factor 2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-human FGF2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-human FGF2 Antibody Images
Click the images to enlarge.
All lanes: Anti FGF2(RP1006) at 0.5ug/ml
Lane 1: Recombinant Human FGF2 Protein 10ng
Lane 2: Recombinant Human FGF2 Protein 5ng
Lane 3: Recombinant Human FGF2 Protein 2.5ng
Predicted bind size: 17KD
Observed bind size: 17KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,