Anti-Interleukin-4 IL4 Antibody

Boster Bio Anti-Interleukin-4 IL4 Antibody catalog # RP1009. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. Cited in 4 publication(s).

Product Info Summary

SKU: RP1009
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-Interleukin-4 IL4 Antibody

See all IL-4 products

SKU/Catalog Number

RP1009

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Interleukin-4 IL4 Antibody catalog # RP1009. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Interleukin-4 IL4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1009)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Immunogen

E. coli-derived human IL-4 recombinant protein (Position: H25-S153).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1009 is reactive to IL4 in Human

Applications

RP1009 is guaranteed for ELISA, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For IL4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P05112

Gene ID

3565

Gene Name

IL4

Full Name

Interleukin-4

Weight

17.492kDa

Superfamily

IL-4/IL-13 family

Alternative Names

B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra IL4 BCGF-1, BCGF1, BSF-1, BSF1, IL-4 interleukin 4 interleukin-4|B cell growth factor 1|B_cell stimulatory factor 1|binetrakin|interleukin 4 variant 2|lymphocyte stimulatory factor 1|pitrakinra

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL4, check out the IL4 Infographic

IL4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

RP1009 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Upregulated protein arginine methyltransferase 1 by IL-4 increases eotaxin-1 expression in airway epithelial cells and participates in antigen-induced pulmonary %u2026

Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.

Wang Yz, Feng Xg, Shi Qg, Hao Yl, Yang Y, Zhang Am, Kong Qx. Inflammation. 2013 Apr;36(2):337-45. Doi: 10.1007/S10753-012-9551-5. Silencing Of Mir155 Promotes The Production Of Inflammatory Mediators In Guillain-Barr?? Syndrome In Vitro.

Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...

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4 Customer Q&As for Anti-Interleukin-4 IL4 Antibody

Question

My boss were content with the WB result of your anti-IL4 antibody. However we have observed positive staining in oocyte secreted. using this antibody. Is that expected? Could you tell me where is IL4 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-12-30

Answer

According to literature, oocyte does express IL4. Generally IL4 expresses in secreted. Regarding which tissues have IL4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-12-30

Question

We bought anti-IL4 antibody for ELISA on oocyte a few years ago. I am using human, and We are going to use the antibody for IHC next. you antibody examining oocyte as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2018-04-16

Answer

I have checked the website and datasheets of our anti-IL4 antibody and it appears that RP1009 has been validated on human in both ELISA and IHC. Thus RP1009 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-04-16

Question

We have been able to see staining in human blood. Are there any suggestions? Is anti-IL4 antibody supposed to stain blood positively?

Verified Customer

Verified customer

Asked: 2017-11-23

Answer

Based on literature blood does express IL4. Based on Uniprot.org, IL4 is expressed in oocyte, blood, among other tissues. Regarding which tissues have IL4 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-11-23

Question

We are currently using anti-IL4 antibody RP1009 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?

K. Krishna

Verified customer

Asked: 2014-06-19

Answer

The anti-IL4 antibody (RP1009) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-06-19

Conjugation

Size

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RP1009
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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