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- Table of Contents
SYK antibody (A00490-3) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of RT4 cells using anti-SYK antibody (A00490-3).
Overlay histogram showing RT4 cells stained with A00490-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SYK Antibody (A00490-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing RT4 cells stained with A00490-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SYK Antibody (A00490-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-SYK Antibody Picoband®
SYK antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
SYK antibody was positively detected by WB in:
human THP-1 whole cell, human HEL whole cell, human A431 whole cell, human RT4 whole cell, rat spleen tissue, mouse RAW264.7 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SYK antibody was positively detected by IHC in:
human lymphoma tissue, mouse spleen tissue, rat spleen tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SYK antibody was positively detected by FC in:
RT4 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Human
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-SYK Antibody Picoband®
View all SYK Antibodies
View all SYK Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SYK recombinant protein (Position: A10-N635).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated Molecular Weight
72.1 kDa
Background of SYK
Spleen tyrosine kinase, also known as Syk, is an enzyme which in humans is encoded by the SYK gene. It is mapped to 9q22.2. This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Cite This Product
Anti-SYK Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00490-3)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Rabbit
93%
High Homology
Dog
93%
High Homology
Mouse
93%
High Homology
Rat
92%
High Homology
Cow
92%
High Homology
Sheep
92%
High Homology
Zebrafish
70%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SYK antibody
Immunohistochemistry (IHC) protocol for SYK antibody
Flow Cytometry (FC) protocol for SYK antibody
Review
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1 Review For Anti-SYK Antibody Picoband®
This antibody is highly specific and efficient, suitable for Western blot detection of Anti-SYK Antibody protein in HT22 cells, with only minor nonspecific bands observed.
| SKU | A00318-1 |
|---|---|
| Application | Western Blot |
| Sample | Mouse hippocampal tissue |
| Sample Processing Description | Mouse brain tissues were lysed in RIPA buffer containing protease inhibitors at 4°C for 2 hours, followed by centrifugation to collect the supernatant. Protein concentration was then determined, and after adjusting to the desired concentration, samples were mixed with 5× protein loading buffer and denatured by heating for 10 minutes. Fifteen microliters of each sample were loaded per lane for electrophoresis. |
| Other Reagents | Blocking buffer |
| Primary Antibody | PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband® |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP Goat Anti-Rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | The figure shows a schematic of the WB results for PIK3R1 and the loading control β-actin in brain tissues from normal mice, model mouse, and mouse treated with high and low doses of AB drug. Although the expression differences between experimental groups are not obvious, the WB results with this antibody are still clear and well-defined. |
Yu Tian, Institute of Materia Medica, Beijing
Verified customer
Submitted 2025-12-25
Product Promise
We promise Anti-SYK Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00490-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026