SKU RP1025
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications ELISA, WB

Overview

Product Name Anti-NGF Antibody
SKU/Catalog Number RP1025
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Beta-nerve growth factor(NGF) detection. Tested with WB, ELISA in Human.
Cite This Product Anti-NGF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1025)
Host Rabbit
Immunogen E. coli-derived human NGFB recombinant protein(Position: S122-A241).
Reactivity Human

Assay Details

Assay Dilutions Overview

Western blot, 0.1-0.5μg/ml, Human, -
ELISA , 0.1-0.5μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

/antibody/rp1025 1 WB anti ngf ngf beta antibody.jpg

Anti-human NGF antibody, RP1025, Western blotting
Lane 1: Recombinant Human NGFB Protein 10ng
Lane 2: Recombinant Human NGFB Protein 5ng
Lane 3: Recombinant Human NGFB Protein 2

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P01138
Gene Name NGF
Protein Name Beta-nerve growth factor
Alternative Names Beta-nerve growth factor;Beta-NGF;NGF;NGFB;
Subcellular Localization Secreted.
Molecular Weight 26959 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). .
Background Nerve growth factor is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. the nucleotide sequence of human and mouse beta-NGF are very similar. the beta-subunits of nerve growth factor(NGFB) have been assigned to mouse chromosome 3 and human chromosome 1p22. The human gene for the beta subunit of nerve growth factor is located on the proximal short arm of chromosome 1. A mutation in the nerve growth factor beta gene(NGFB) causes loss of pain perception.

Order Product (RP1025)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1054 $240
100ug+Free Biotin Secondary BA1003 $240

USD $240

Ships in 5-7 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

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Publications

Polyaniline promotes peripheral nerve regeneration by enhancement of the brain-derived neurotrophic factor and ciliary neurotrophic factor expression and activation of the ERK1/2/MAPK signaling pathway
Nerve growth factor and inducible nitric oxide synthase expression in the mesencephalon and diencephalon, as well as visual- and auditory-related nervous tissues, in a macaque model of type 2 diabetes
Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury
Expression of nerve growth factor in the prostate of male rats in response to chronic stress and sympathetic denervation
Effects of minocycline on the expression of NGF and HSP70 and its neuroprotection role following intracerebral hemorrhage in rats
Associations between proinflammatory cytokines in the synovial fluid and radiographic grading and pain-related scores in 47 consecutive patients with osteoarthritis of the knee
Sun Fj, Guo W, Zheng Dh, Zhang Cq, Li S, Liu Sy, Yin Q, Yang H, Shu Hf. J Mol Neurosci. 2013 Jan;49(1):182-93. Doi: 10.1007/S12031-012-9878-2. Epub 2012 Aug 31. Increased Expression Of Trpv1 In The Cortex And Hippocampus From Patients With Mesial ...
Huang S, Zhang X, Xu L, Li Q, Zhao Q. Exp Ther Med. 2014 Oct;8(4):1237-1240. Epub 2014 Jul 21. Expression Of Nerve Growth Factor In The Prostate Of Male Rats In Response To Chronic Stress And Sympathetic Denervation.
Li Jf, Shu Jc, Tang Sh, Deng Ym, Fu My, Lv X. Mol Med Rep. 2013 Sep;8(3):813-7. Doi: 10.3892/Mmr.2013.1590. Epub 2013 Jul 16. ??-Nerve Growth Factor Attenuates Hepatocyte Injury Induced By D-Galactosamine In Vitro Via Trka Ngfr.
Luo Q, Liu W, Chen J, Wang M, Zeng W, Chen Z, Cheng A. Neural Regen Res. 2012 Feb 5;7(4):302-7. Doi: 10.3969/J.Issn.1673-5374.2012.04.011. Nerve Growth Factor And Inducible Nitric Oxide Synthase Expression In The Mesencephalon And Diencephalon, As...
Li C, Ma Y, Yi K, Wang C, Li W, Liu Z, Sun L, Chen S, Yu J, Li H, Chen L, Zhou X. Anim Reprod Sci. 2014 Oct;149(3-4):117-23. Doi: 10.1016/J.Anireprosci.2014.06.030. Epub 2014 Jul 2. The Interactions Between Nerve Growth Factor And Gonadotrophins I...
Lu Ql, Liu J, Zhu Xl, Xu Wj. J Huazhong Univ Sci Technolog Med Sci. 2014 Jun;34(3):359-62. Doi: 10.1007/S11596-014-1283-3. Epub 2014 Jun 18. Expression Of Nerve Growth Factor And Hypoxia Inducible Factor-1?? And Its Correlation With Angiogenesis In...

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to nerve growth factor receptor antibody, ngfr antibody, p75ntr antibody