Anti-Beta-nerve growth factor NGF Antibody

Boster Bio Anti-Beta-nerve growth factor NGF Antibody catalog # RP1025. Tested in ELISA, WB applications. This antibody reacts with Human. Cited in 11 publication(s).

Product Info Summary

SKU: RP1025
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-Beta-nerve growth factor NGF Antibody

See all beta-NGF products

SKU/Catalog Number

RP1025

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Beta-nerve growth factor NGF Antibody catalog # RP1025. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Beta-nerve growth factor NGF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1025)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Immunogen

E. coli-derived human NGFB recombinant protein (Position: S122-A241).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

RP1025 is reactive to NGF in Human

Applications

RP1025 is guaranteed for ELISA, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, -
ELISA , 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For NGF (Source: Uniprot.Org, NCBI)

Uniprot ID

P01138

Gene ID

4803

Gene Name

NGF

Full Name

Beta-nerve growth factor

Weight

26.959kDa

Superfamily

NGF-beta family

Alternative Names

Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428 NGF Beta-NGF, HSAN5B, NGF nerve growth factor beta-nerve growth factor|nerve growth factor (beta polypeptide)|nerve growth factor, beta subunit|pro-nerve growth factor long

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NGF, check out the NGF Infographic

NGF infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NGF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

RP1025 has been cited in 11 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Serum EGF and NGF levels of patients with brain injury and limb fracture

Urinary brain-derived neurotrophic factor: a potential biomarker for objective diagnosis of overactive bladder

Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury

Effects of minocycline on the expression of NGF and HSP70 and its neuroprotection role following intracerebral hemorrhage in rats

Associations between proinflammatory cytokines in the synovial fluid and radiographic grading and pain-related scores in 47 consecutive patients with osteoarthritis of the knee

Sun Fj, Guo W, Zheng Dh, Zhang Cq, Li S, Liu Sy, Yin Q, Yang H, Shu Hf. J Mol Neurosci. 2013 Jan;49(1):182-93. Doi: 10.1007/S12031-012-9878-2. Epub 2012 Aug 31. Increased Expression Of Trpv1 In The Cortex And Hippocampus From Patients With Mesial ...

Huang S, Zhang X, Xu L, Li Q, Zhao Q. Exp Ther Med. 2014 Oct;8(4):1237-1240. Epub 2014 Jul 21. Expression Of Nerve Growth Factor In The Prostate Of Male Rats In Response To Chronic Stress And Sympathetic Denervation.

Li Jf, Shu Jc, Tang Sh, Deng Ym, Fu My, Lv X. Mol Med Rep. 2013 Sep;8(3):813-7. Doi: 10.3892/Mmr.2013.1590. Epub 2013 Jul 16. ??-Nerve Growth Factor Attenuates Hepatocyte Injury Induced By D-Galactosamine In Vitro Via Trka Ngfr.

Luo Q, Liu W, Chen J, Wang M, Zeng W, Chen Z, Cheng A. Neural Regen Res. 2012 Feb 5;7(4):302-7. Doi: 10.3969/J.Issn.1673-5374.2012.04.011. Nerve Growth Factor And Inducible Nitric Oxide Synthase Expression In The Mesencephalon And Diencephalon, As...

Li C, Ma Y, Yi K, Wang C, Li W, Liu Z, Sun L, Chen S, Yu J, Li H, Chen L, Zhou X. Anim Reprod Sci. 2014 Oct;149(3-4):117-23. Doi: 10.1016/J.Anireprosci.2014.06.030. Epub 2014 Jul 2. The Interactions Between Nerve Growth Factor And Gonadotrophins I...

Lu Ql, Liu J, Zhu Xl, Xu Wj. J Huazhong Univ Sci Technolog Med Sci. 2014 Jun;34(3):359-62. Doi: 10.1007/S11596-014-1283-3. Epub 2014 Jun 18. Expression Of Nerve Growth Factor And Hypoxia Inducible Factor-1?? And Its Correlation With Angiogenesis I...

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16 Customer Q&As for Anti-Beta-nerve growth factor NGF Antibody

Question

Our lab used your anti-NGF antibody for WB on brain in a previous experiment. I am using human, and We intend to use the antibody for ELISA next. We want examining brain as well as eye in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2020-04-27

Answer

I viewed the website and datasheets of our anti-NGF antibody and I see that RP1025 has been tested on human in both WB and ELISA. Thus RP1025 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-04-27

Question

Is this RP1025 anti-NGF antibody reactive to the isotypes of NGF?

Verified Customer

Verified customer

Asked: 2020-04-27

Answer

The immunogen of RP1025 anti-NGF antibody is E. coli-derived human NGFB recombinant protein(Position: S122-A241). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-27

Question

Can you help my question with product RP1025, anti-NGF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1025 anti-NGF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-04

Question

We are currently using anti-NGF antibody RP1025 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-21

Answer

The anti-NGF antibody (RP1025) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-21

Question

Here is the WB image, lot number and protocol we used for eye using anti-NGF antibody RP1025. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-28

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-NGF antibody RP1025. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-02

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-02

Question

I was wanting to use your anti-NGF antibody for ELISA for human eye on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human eye identification?

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

It shows on the product datasheet, RP1025 anti-NGF antibody has been validated for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human eye in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-07-03

Question

My boss were well pleased with the WB result of your anti-NGF antibody. However we have observed positive staining in eye secreted. using this antibody. Is that expected? Could you tell me where is NGF supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

From literature, eye does express NGF. Generally NGF expresses in secreted. Regarding which tissues have NGF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2374737
Eye, Pubmed ID: 15489334
Leukocyte, Pubmed ID: 2025430

Boster Scientific Support

Answered: 2019-06-27

Question

We have observed staining in human leukocyte. What should we do? Is anti-NGF antibody supposed to stain leukocyte positively?

S. Gonzalez

Verified customer

Asked: 2019-04-12

Answer

Based on literature leukocyte does express NGF. Based on Uniprot.org, NGF is expressed in cartilage tissue, brain, eye, leukocyte, among other tissues. Regarding which tissues have NGF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2374737
Eye, Pubmed ID: 15489334
Leukocyte, Pubmed ID: 2025430

Boster Scientific Support

Answered: 2019-04-12

Question

I see that the anti-NGF antibody RP1025 works with ELISA, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-06-19

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-06-19

Question

Would anti-NGF antibody RP1025 work for ELISA with eye?

Verified Customer

Verified customer

Asked: 2017-11-09

Answer

According to the expression profile of eye, NGF is highly expressed in eye. So, it is likely that anti-NGF antibody RP1025 will work for ELISA with eye.

Boster Scientific Support

Answered: 2017-11-09

Question

Would anti-NGF antibody RP1025 work on horse WB with leukocyte?

Verified Customer

Verified customer

Asked: 2017-09-13

Answer

Our lab technicians have not tested anti-NGF antibody RP1025 on horse. You can run a BLAST between horse and the immunogen sequence of anti-NGF antibody RP1025 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse leukocyte in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-09-13

Question

I was wanting to use to test anti-NGF antibody RP1025 on human eye for research purposes, then I may be interested in using anti-NGF antibody RP1025 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-08-15

Answer

The products we sell, including anti-NGF antibody RP1025, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-08-15

Question

Is a blocking peptide available for product anti-NGF antibody (RP1025)?

E. Baker

Verified customer

Asked: 2014-08-11

Answer

We do provide the blocking peptide for product anti-NGF antibody (RP1025). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2014-08-11

Question

Would RP1025 anti-NGF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

K. Jha

Verified customer

Asked: 2014-02-11

Answer

You can see on the product datasheet, RP1025 anti-NGF antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-02-11

Question

Is there a BSA free version of anti-NGF antibody RP1025 available?

B. Mangal

Verified customer

Asked: 2013-11-20

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-NGF antibody RP1025 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-11-20

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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RP1025
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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