Product Info Summary
SKU: | A01245-2 |
---|---|
Size: | 1mg |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Dot blot |
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Product info
Product Name
Anti-Human Serum Albumin Antibody Fluorescein Conjugated
SKU/Catalog Number
A01245-2
Size
1mg
Form
Lyophilized
Description
Boster Bio Anti-Human Serum Albumin Antibody Fluorescein Conjugated (Catalog # A01245-2). Tested in Dot blot applications. This antibody reacts with Human.
Storage & Handling
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Cite This Product
Anti-Human Serum Albumin Antibody Fluorescein Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01245-2)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Polyethylene Glycol (PEG-8000), 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Human Serum Albumin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01245-2 is reactive to ALB in Human
Applications
A01245-2 is guaranteed for Dot blot Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
69.367kDa
Background of ALB
Anti-Human serum Fluorescein detects Human serum proteins. Serum proteins are those proteins remaining in portion of plasma after coagulation of blood, during which process the plasma protein fibrinogen is converted to fibrin and remains behind in the clot. Anti-Human serum is ideal for investigators involved in Cell Signaling, Cellular Biology and Signal Transduction research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Fluorochrome Protein Value: 3.7
IF Microscopy: User optimized
WB: User optimized
Validation Images & Assay Conditions
Click image to see more details
Dot Blot results of Rabbit Anti-Human Serum Albumin Antibody Fluorescein Conjugate.Dots are Human Serum Albumin: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none.Secondary Antibody: Rabbit Anti-Human Serum Albumin Antibody FITC at 1ug/mL in 1% Fish Gel 1hr RT.Imaged with BioRad ChemiDoc, Fluorescein filter.
Protein Target Info & Infographic
Gene/Protein Information For ALB (Source: Uniprot.org, NCBI)
Gene Name
ALB
Full Name
Serum albumin
Weight
69.367kDa
Superfamily
ALB/AFP/VDB family
Alternative Names
ALB; Albumin; cell growth inhibiting protein 42; DKFZp779N1935; growth-inhibiting protein 20; PRO0883; PRO0903; PRO1341; serum albumin ALB HSA, PRO0883, PRO0903, PRO1341 albumin albumin|serum albumin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALB, check out the ALB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human Serum Albumin Antibody Fluorescein Conjugated (A01245-2)
Hello CJ!
A01245-2 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Pro%u2011angiogenic microRNA%u2011296 upregulates vascular endothelial growth factor and downregulates Notch1 following cerebral ischemic injury
The Healing Effects of Conditioned Medium Derived from Mesenchymal Stem Cells on Radiation-Induced Skin Wounds in Rats
Resveratrol attenuates the progress of liver fibrosis via the Akt/nuclear factor-%u03BAB pathways
VTIQ evaluates antitumor effects of NET%u20111 siRNA by UTMD in HCC xenograft models
Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion
Non-tumor tissue derived interleukin-17B activates IL-17RB/AKT/%u03B2-catenin pathway to enhance the stemness of gastric cancer
Adipose-derived stem cells from the breast
Clinicopathologic and prognostic significance of VEGF, JAK2 and STAT3 in patients with nasopharyngeal carcinoma
Overexpression of estrogen receptor beta alleviates the toxic effects of beta-amyloid protein on PC12 cells via non-hormonal ligands
Disrupted fibroblastic reticular cells and interleukin%u20117 expression in tumor draining lymph nodes
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-Human Serum Albumin Antibody Fluorescein Conjugated
Question
I see that the anti-Human Serum Albumin antibody Fluorescein Conjugated A01245-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-04
Question
Would A01245-2 anti-Human Serum Albumin antibody Fluorescein Conjugated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
You can see on the product datasheet, A01245-2 anti-Human Serum Albumin antibody Fluorescein Conjugated as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-02
Question
Do you have a BSA free version of anti-Human Serum Albumin antibody Fluorescein Conjugated A01245-2 available?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human Serum Albumin antibody Fluorescein Conjugated A01245-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-24
Question
Is a blocking peptide available for product anti-Human Serum Albumin antibody Fluorescein Conjugated (A01245-2)?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
We do provide the blocking peptide for product anti-Human Serum Albumin antibody Fluorescein Conjugated (A01245-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-23
Question
I was wanting to use your anti-Human Serum Albumin antibody Fluorescein Conjugated for WB for human colon carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human colon carcinoma identification?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
You can see on the product datasheet, A01245-2 anti-Human Serum Albumin antibody Fluorescein Conjugated has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human colon carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-18
Question
See attached the WB image, lot number and protocol we used for colon carcinoma using anti-Human Serum Albumin antibody Fluorescein Conjugated A01245-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-23
Question
Would anti-Human Serum Albumin antibody Fluorescein Conjugated A01245-2 work on goat WB with liver?
T. Jha
Verified customer
Asked: 2017-02-06
Answer
Our lab technicians have not tested anti-Human Serum Albumin antibody Fluorescein Conjugated A01245-2 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Human Serum Albumin antibody Fluorescein Conjugated A01245-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-02-06