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- Table of Contents

ALB antibody (M01245) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
ALB antibody was positively detected by WB in:
rat blood, rat liver tissue, mouse blood, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ALB antibody was positively detected by IHC in:
human liver tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
69.4 kDa
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| SKU | M01245 |
|---|---|
| Application | Western Blot |
| Sample | Human keratinocytes isolated from skin |
| Sample Processing Description | Keratinocytes isolated from normal skin tissue. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | Albumin Rabbit Monoclonal Antibody |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP Goat Anti-Rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | Human serum albumin (ALB) is the most abundant protein in plasma, with key functions including maintaining colloid osmotic pressure, transporting substances, providing antioxidant activity, and regulating inflammation. Clinically, ALB is widely used to treat hypoalbuminemia, shock, burns, and other conditions. ALB was long thought to be secreted exclusively by hepatocytes, but recent studies suggest that other cell types may also be capable of synthesizing it. The purpose of this experiment was to verify whether human skin keratinocytes can synthesize ALB. The results show that skin keratinocytes are indeed capable of producing ALB. |
Xianyong Zhou, Hangzhou Jiexiao Testing Technology
Verified customer
Submitted 2025-12-08
We promise Anti-Albumin Rabbit Monoclonal Antibody performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.