Anti-Albumin Rabbit Monoclonal Antibody

FCGR2B were up-regulated in hippocampus of DM mice. A qRT-PCR was performed to detect the expression of ALB, AREG and FCGR2B mRNA expression in hippocampus of mice. B Western blot was conducted to detect the ALB, AREG and FCGR2B protein expression in hippocampus of mice. C IHC assay was employed to examine the ALB, AREG and FCGR2B protein expression in hippocampus of mice. D IF staining was utilized to detect the expression of FCGR2B and NeuN in hippocampus of mice. E Western blot was performed to detect the SHC1 protein expression in hippocampus of mice. F IF staining was performed to detect the expression of SHC1 and NeuN in hippocampus of mice. G Western blot was used to detect the p-PI3K and p-AKT protein expression in hippocampus of mice. *** P < 0.001 Full size image
Index in PubMed under a CC BY license. PMID: 40537751

Western blot analysis of Albumin using anti-Albumin antibody (M01245).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat blood lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse blood lysates,
Lane 4: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Albumin antigen affinity purified monoclonal antibody (M01245) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Albumin at approximately 69 kDa. The expected band size for Albumin is at 69 kDa.

Immunohistochemical analysis of paraffin-embedded human liver, using Serum Albumin Antibody.

Western blot analysis of Albumin using anti-Albumin antibody (M01245).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1-2: Human keratinocytes isolated from skin.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Albumin antigen affinity purified monoclonal antibody (M01245) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate with ChemiDoc MP system. A specific band was detected for Albumin at approximately 69 kDa. The expected band size for Albumin is at 69 kDa.