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Product Info Summary
| SKU: | A03671-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ICAD DFFA Antibody
View all DFF45/ICAD Antibodies
SKU/Catalog Number
A03671-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ICAD DFFA Antibody catalog # A03671-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ICAD DFFA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03671-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
5A6
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human ICAD. at AA rangle: 120-200
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03671-1 is reactive to DFFA in Human, Mouse, Rat
Applications
A03671-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
36.522kDa
Background of DFF45/ICAD
CAND1 is also known as TIP120A, and TATA-binding protein-interacting protein 120A. The SCF complex consists of the invariable components Skp1, Cul1, and Rbx1 as well as a variable F-box protein, and functions as an E3 ubiquitin ligase. E3 ubiquitin ligases regulate various physiological processes. CAND1 binds to Cul1 and potentially regulates the SCF complex. CAND1 physically associates with Cul1 in the nucleus and this interaction is mediated by a central region of Cul1 distinct from its binding sites for Skp1 and Rbx1. CAND1 selectively binds to unneddylated CUL1 and is dissociated by CUL1 neddylation. CAND1 forms a ternary complex with CUL1 and ROC1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of 3T3 cells using ICAD Polyclonal antibody.
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Western Blot (WB) analysis of specific cells using ICAD Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For DFFA (Source: Uniprot.org, NCBI)
Gene Name
DFFA
Full Name
DNA fragmentation factor subunit alpha
Weight
36.522kDa
Alternative Names
DFF1; DFF1DNA fragmentation factor 45 kDa subunit; DFF45; DFF45DNA fragmentation factor subunit alpha; DFF-45DNA fragmentation factor, 45 kD, alpha polypeptide; DFFA; DNA fragmentation factor, 45kDa, alpha polypeptide; ICAD; Inhibitor of CAD DFFA DFF-45, DFF1, ICAD DNA fragmentation factor subunit alpha DNA fragmentation factor subunit alpha|DFF45|DNA fragmentation factor 45 kDa subunit|DNA fragmentation factor, 45kDa, alpha polypeptide|inhibitor of CAD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DFFA, check out the DFFA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DFFA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ICAD DFFA Antibody (A03671-1)
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