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Product Info Summary
| SKU: | A02652-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-IFIT1 Antibody
SKU/Catalog Number
A02652-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IFIT1 Antibody (Catalog # A02652-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
IFIT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-IFIT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02652-1)
Host
Rabbit
Contents
IFIT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
IFIT1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human IFIT1. The immunogen is located within amino acids 290 - 340 of IFIT1.
Cross-reactivity
IFIT1 antibody is human, mouse and rat reactive. At least three isoforms of IFIT1 are known to exist. This antibody is predicted to not cross-react with other members of the IFIT protein family.
Reactive Species
A02652-1 is reactive to IFIT1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
55.4 kDa
Background of IFIT1
The interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) protein is a member of a family of tetratricopeptide repeat-containing proteins whose transcription is upregulated by interferons, virus infection, and molecular patterns such as dsRNA or lipopolysaccharides (1,2). These proteins have been suggested to induce anti-viral cellular activities in response to infection (2). Together with the interferon-induced transmembrane protein 1 (IFITM1), IFIT1 inhibits the replication of the Hepatitis C virus, suggesting that it may be useful in the development of therapeutic treatments (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02652-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IFIT1 antibody can be used for detection of IFIT1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of IFIT1 in rat small intestine tissue lysate with IFIT1 antibody at 1 μg/ml.
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Immunohistochemistry of IFIT1 in human small intestine tissue with IFIT1 antibody at 5 μg/mL.
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Immunofluorescence of IFIT1 in human small intestine tissue with IFIT1 antibody at 20 μg/mL.
Specific Publications For Anti-IFIT1 Antibody (A02652-1)
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