Human IGF-1 ELISA Kit PicoKine™

Human IGF-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IGF1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. Cited in 8 publication(s).

Product Info Summary

SKU: EK0376
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Human IGF-1 ELISA Kit PicoKine™

See all IGF-I/IGF-1 products

SKU/Catalog Number



96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?


Human IGF-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IGF1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human IGF-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0376)

Clonality of Antibodies

See Datasheet for details


Expression system for standard: E.coli; Immunogen sequence: G49-A118


<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with IGF-2.

Reactive Species

EK0376 is reactive to IGF1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK0376 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0376-CAP Anti-Human IGF1 Pre-coated 96-well strip microplate 1
EK0376-ST Human IGF1 Standard 2 vials, 10 ng/tube
EK0376-DA Human IGF1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IGF-I/IGF-1, Dilution ratio of 1:4, concentration in serum is around 2-6ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IGF-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Mean (pg/ml)2151065356628411544861
Standard deviation8.8245.80164.0414.4868.09325.69
CV (%)4.1%4.3%4.6%5.1%5.9%6.7%


We ensure reproducibility by testing three samples with differing concentrations of IGF-I/IGF-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 121521119118820111.885.9%
Sample 21065109111341073109026.682.4%
Sample 335663691336933073483153.394.4%
*number of samples for each test n=16.

Gene/Protein Information For IGF1 (Source: Uniprot.Org, NCBI)

Uniprot ID


Gene ID


Gene Name


Full Name

Insulin-like growth factor I




insulin family

Alternative Names

IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C IGF1 IGF, IGF-I, IGFI, MGF insulin like growth factor 1 insulin-like growth factor I|insulin-like growth factor 1 (somatomedin C)|insulin-like growth factor IB|mechano growth factor|somatomedin-C

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IGF1, check out the IGF1 Infographic

IGF1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IGF1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0376 has been cited in 8 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

IGF-1 promotes the development and cytotoxic activity of human NK cells
Species: Human

Dorneles GP,da Silva IM,Santos MA,Elsner VR,Fonseca SG,Peres A,Romão PRT. Immunoregulation induced by autologous serum collected after acute exercise in obese men: a randomized cross-over trial.Sci Rep. 2020 Dec 10;10(1):21735.doi:10.1038/s41598-020-78750
Species: Human

Insulin-like growth factor-1 promotes the proliferation and odontoblastic differentiation of human dental pulp cells under high glucose conditions

IGF-1 promotes the development and cytotoxic activity of human NK cells

Regulation of signaling pathways downstream of IGF-I/insulin by androgen in skeletal muscle of glucocorticoid-treated rats

Resistance to multikinase inhibitor actions mediated by insulin like growth factor-1

Cai B, Li X, Wang Y, Liu Y, Yang F, Chen H, Yin K, Tan X, Zhu J, Pan Z, Wang B, Lu Y. PLoS One. 2013 May 7;8(5):e63561. doi: 10.1371/journal.pone.0063561. Print 2013. Apoptosis of bone marrow mesenchymal stem cells caused by homocysteine via acti...

Chai H, Guo L, Wang X, Fu Y, Guan J, Tan L, Ren L, Yang K. J Mater Sci Mater Med. 2011 Nov;22(11):2525-35. Doi: 10.1007/S10856-011-4427-Z. Epub 2011 Aug 26. Antibacterial Effect Of 317L Stainless Steel Contained Copper In Prevention Of Implant-Rel...

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10 Customer Q&As for Human IGF-1 ELISA Kit PicoKine™


Q: how many samples can be assayed in a Picokine® ELISA Kit?

J. Bhasin

Verified customer

Asked: 2020-11-13


A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-11-13


Would it would work with their bear samples?

Verified customer

Asked: 2020-09-25


The animal species trying to measure IGF1 concentrations is the Asiatic black bear (Ursus thibetanus Japonicus), and the exact protein sequencing has not been reported. In the case of closely related species, brown and polar bears have genomic information available to the public.

Boster Scientific Support

Answered: 2020-10-25


Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

S. Anand

Verified customer

Asked: 2020-07-23


A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: You can also consult our article on ELISA data analysis:

Boster Scientific Support

Answered: 2020-07-23


I am doing a research about human IGF-1. I would like to know whether EK0376 Human IGF-1 ELISA Kit can detect free of total IGF-1. I really need your help. Thank you

Verified Customer

Verified customer

Asked: 2020-05-08


The EK0376 Human IGF-1 ELISA Kit is designed to measure free IGF1. IGF-1 is usually bound to IGFBPs in the serum/plasma, so if you would like to measure total IGF1, it is advisable to release the analytes by performing an extraction procedure such as acid-ethanol treatment.

Boster Scientific Support

Answered: 2020-05-08


I am interested purchasing EK0376 Human IGF-1 ELISA Kit for serum or plasma samples. I read the protocol calls for pre-treatment of samples to separate IGF1 from the IGF1 Binding Proteins in order to accurately measure total IGF1. Is it possible not performing the extraction step and only measure free IGF1 (not initially bound to IGF1BPs) in serum or plasma? ? or have you ever tried it?

Verified Customer

Verified customer

Asked: 2019-12-23


Hello; the purpose of the extraction step is to increase the signal, as the antibody EK0376 Human IGF-1 ELISA Kit recognition is apparently blocked by the binding protein interfering with the epitopes that one or both of the antibodies bind to. Probably some signal will be obtained even without the extraction step, but the EK0376 Human IGF-1 ELISA Kit will not be able to differentiate signal contributed by free EGF1 from the signal contributed by EGF1 in complex with binding protein.

Boster Scientific Support

Answered: 2019-12-23


Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement IGF-I using the EK0376 Human IGF-I Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-10-08


A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2018-10-08


Q: can you recommend the dilution ratio of serum samples for detection of IGF-I in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

J. Babu

Verified customer

Asked: 2018-03-31


A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IGF-I ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here:
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-03-31


Q: how to proceed with the analysis of ELISA data? I have performed IGF-I level in plasma.

Verified Customer

Verified customer

Asked: 2017-08-22


A: please read this article on ELISA data analysis. Boster also provides a free tool that you can use to analyze ELISA data.

Boster Scientific Support

Answered: 2017-08-22


Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2015-12-02


A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2015-12-02


Q: if the enzyme conjugated IGF-I antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IGF-I antigen?

Verified Customer

Verified customer

Asked: 2015-03-28


A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2015-03-28


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