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Product Info Summary
| SKU: | M01816-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-IKBKE Mouse Monoclonal Antibody [Clone ID: OTI3C5]
SKU/Catalog Number
M01816-1
Size
100 µl
Description
Boster Bio IKBKE mouse monoclonal antibody, clone OTI3C5 (formerly 3C5). Catalog# M01816-1. Tested in IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IKBKE Mouse Monoclonal Antibody [Clone ID: OTI3C5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01816-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3C5
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 474-716 of human IKBKE (NP_054721) produced in E.coli.
Reactive Species
M01816-1 is reactive to IKBKE in Human
Calculated molecular weight
80.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01816-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IHC 1:150
Validation Images & Assay Conditions
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IKBKE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IKBKE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IKBKE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-IKBKE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-IKBKE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IKBKE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-IKBKE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IKBKE mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IKBKE (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IKBKE (1:2000).
Specific Publications For Anti-IKBKE Mouse Monoclonal Antibody [Clone ID: OTI3C5] (M01816-1)
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