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Product Info Summary
| SKU: | A01144 |
|---|---|
| Size: | 1mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-IL-1 Alpha Antibody
SKU/Catalog Number
A01144
Size
1mg
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-IL-1 Alpha Antibody (Catalog # A01144). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-IL-1 Alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01144)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1a produced in E.coli. The MW of the recombinant IL-1a was 17,000. This is the cleavage site generated by the IL-1b converting enzyme (ICE, capase-1).
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
A01144 is reactive to IL1A in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
30.6 kDa
Background of IL1A
Interleukin 1 alpha is produced by activated macrophages. Anti-IL-1 alpha antibody is ideal for investigators involved in Immunology research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01144 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:1,000 - 1:5,000
Flow Cytometry: User optimized
IHC: 1:100 to 1:200
WB: 1:500 - 1:2,000
Neutralization: User optimized
This IgG fraction antibody of anti-Human IL-1a has been tested for use in immunoblotting. This antibody is suitable for use in ELISA, neutralizations, immunohistochemistry, and flow cytometry. It recognizes the 17,000 MW mature IL-1a. Reactivity in other immunoassays is unknown.
Validation Images & Assay Conditions
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Western Blot of Rabbit Anti-Human IL-1 alpha Antibody. Lane 1: Opal Prestained Molecular Weight Ladder . Lane 2: IL-1 alpha [50ng]. Lane 3: MEF WC lysate [10ng] . Lane 4: MEF LPS stimulated [10ng]. Blocking: BlockOut Buffer for 30min at RT. Primary Antibody: Anti-IL-1a at 1µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP at 1:70, 000 for 30min at RT.
Specific Publications For Anti-IL-1 Alpha Antibody (A01144)
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4 Customer Q&As for Anti-IL-1 Alpha Antibody
Question
We are currently using anti-IL-1 Alpha antibody A01144 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
The anti-IL-1 Alpha antibody (A01144) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-06
Question
I have attached the WB image, lot number and protocol we used for smooth muscle tissue using anti-IL-1 Alpha antibody A01144. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for smooth muscle tissue using anti-IL-1 Alpha antibody A01144. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-05-14
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-14
Question
I have a question about product A01144, anti-IL-1 Alpha antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Parker
Verified customer
Asked: 2018-02-22
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01144 anti-IL-1 Alpha antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-22
