SKU | EK0389 |
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Size | 96wells/kit, with removable strips. |
Reactivity | Human |
Sample Type | cell culture supernates, serum and plasma( heparin, EDTA, citrate). |
Sensitivity | <0.5pg/ml |
Assay Range | 4.7pg/ml-300pg/ml |
Overview
Product Name | Human IL-1 Alpha PicoKine™ ELISA Kit |
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SKU/Catalog Number | EK0389 |
Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
Size | 96wells/kit, with removable strips. |
Description | Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-1 alpha. 96wells/kit, with removable strips. |
Cite This Product | Human IL-1 Alpha PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0389) |
Sample Type | cell culture supernates, serum and plasma( heparin, EDTA, citrate)..
Anticoagulant(s): heparin, EDTA or citrate
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants thata are not specified above or contact us to check for feasibility. |
Sensitivity | <0.5pg/ml |
Assay Range | 4.7pg/ml-300pg/ml |
Immunogen | Expression system for standard: E.coli, Immunogen sequence: S113-A271 |
Reactivity | Human |
Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
Antibody Clonalities | Capture Antibody | Detection Antibody: monoclonal antibody from mouse|polyclonal antibody from goat |
Assay Details
Kit Components
Catalog number | Description | Quantity |
EK0389-CAP | 96-well plate precoated with anti-Human IL1A antibody | 1 |
EK0389-ST | lyophilized recombinant Human IL1A standard | 10ng/tube |
EK0389-DA | biotinylated anti-Human IL1A antibody | 130ul |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
AR1106-1 | sample diluent buffer | 30ml |
AR1106-2 | antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Materials Required But Not Provided
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Typical Data Obtained from Human IL-1 Alpha PicoKine™ ELISA Kit
(TMB reaction incubate at 37°C for 15-20min)
Concentration(pg/ml) | 0 | 4.7 | 9.4 | 18.8 | 37.5 | 75 | 150 | 300 |
O.D. | 0.041 | 0.097 | 0.162 | 0.367 | 0.582 | 0.866 | 1.335 | 2.133 |
Intra/Inter Assay Precision
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 14 | 52 | 99 | 15 | 49 | 103 |
Standard deviation | 0.98 | 2.49 | 4.35 | 1.06 | 2.4 | 6.07 |
CV(%) | 7% | 4.8% | 4.4% | 7.1% | 4.9% | 5.9% |
Reproducibility
Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 14 | 15 | 15 | 13 | 14 | 0.82 | 5.8% |
Sample 2 | 52 | 48 | 50 | 48 | 49 | 1.65 | 3.3% |
Sample 3 | 99 | 98 | 112 | 102 | 102 | 5.53 | 5.4% |
*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.
Target Info
Protein Target Info (Source: Uniprot.org)
Uniprot Id | P01583 |
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Gene Name | IL1A |
Protein Name | Interleukin-1 alpha |
Alternative Names | Interleukin-1 alpha;IL-1 alpha;Hematopoietin-1;IL1A;IL1F1; |
Subcellular Localization | Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. |
Molecular Weight | 30607 MW |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
Protein Function | Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. |
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Research Areas | Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology *You can search these to find other products in these research areas. |
Background | Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant human IL-1alpha with the molecular mass of 18kDa. |
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Customer Q&As
• Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed.
• If no signal is produced, then you can work on the tissue sample by using the kit.
The 1XTBS can be used if the pH value falls in the range of 7.2-7.6.
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