Overview
| Product Name |
Human IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine™
See more IL-1 alpha/IL-1F1 products |
| Catalog# |
EK0389 |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Human IL-1 Alpha ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL1A in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).. Sensitivity: 0.5pg/ml. |
| Cite This Product |
Human IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0389)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). |
| Sensitivity |
<0.5 pg/ml |
| Assay Range |
4.7 pg/ml - 300 pg/ml |
| Immunogen/Standard |
Expression system for standard: E.coli; Immunogen sequence: S113-A271 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For IL1A (Source: Uniprot.org, NCBI)
| Uniprot Id | P01583 |
|---|
| NCBI Gene Id | 3552 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | IL1A |
|---|
| Protein Name | Interleukin-1 alpha |
|---|
| Superfamily | IL-1 family |
|---|
| Alternative Names | IL-1 alpha/IL-1F1|BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha |
|---|
| Molecular Weight | 30607 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IL1A, check out the IL1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for IL1A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the IL1A infographic
Kit Components And Assay QC Data Details Of Human IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0389-CAP |
Anti-Human IL1A Pre-coated 96-well strip microplate | 1 |
| EK0389-ST | Human IL1A Standard | 2 vials, 10 ng/tube |
| EK0389-DA | Human IL1A Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0389 Human IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 4.7 | 9.4 | 18.8 | 37.5 | 75 | 150 | 300 |
| O.D. | 0.041 | 0.097 | 0.162 | 0.367 | 0.582 | 0.866 | 1.335 | 2.133 |
We measured random samples of Human IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 14 | 52 | 99 | 15 | 49 | 103 |
| Standard deviation | 0.98 | 2.49 | 4.35 | 1.06 | 2.4 | 6.07 |
| CV (%) | 7% | 4.8% | 4.4% | 7.1% | 4.9% | 5.9% |
We measured the performance consistency of Human IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 14 | 15 | 15 | 13 | 14 | 0.82 | 5.8% |
| Sample 2 | 52 | 48 | 50 | 48 | 49 | 1.65 | 3.3% |
| Sample 3 | 99 | 98 | 112 | 102 | 102 | 5.53 | 5.4% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 9
| Effect of etomidate on the oxidative stress response and levels of inflammatory factors from ischemia-reperfusion injury after tibial fracture surgery |
PubMed ID 28450928 |
| Sun X, Shi B, Zheng H, Min L, Yang J, Li X, Liao X, Huang W, Zhang M, Xu S, Zhu Z, Cui H, Liu X. Cell Death Dis. 2018 Feb 15;9(3):260. doi: 10.1038/s41419-018-0303-9. Senescence-associated secretory factors induced by cisplatin in melanoma cells p... |
PubMed ID 29449532 |
| Han X, Wang T, Zhang J, Liu X, Li Z, Wang G, Song Q, Pang D, Ouyang H, Tang X. Biol Open. 2015 Apr 2;4(5):661-5. Doi: 10.1242/Bio.201410900. Apolipoprotein Ciii Regulates Lipoprotein-Associated Phospholipase A2 Expression Via The Mapk And Nf??b Pa... |
PubMed ID 25836672 |
| Wang G, Ji Y, Li Z, Han X, Guo N, Song Q, Quan L, Wang T, Han W, Pang D, Ouyang H, Tang X. Sci Rep. 2014 May 9;4:4905. Doi: 10.1038/Srep04905. Nitro-Oleic Acid Downregulates Lipoprotein-Associated Phospholipase A2 Expression Via The P42/P44 Mapk A... |
PubMed ID 24809325 |
| Li J, Huang M, Shen X. J Diabetes Complications. 2014 Sep-Oct;28(5):662-6. Doi: 10.1016/J.Jdiacomp.2014.06.008. Epub 2014 Jun 19. The Association Of Oxidative Stress And Pro-Inflammatory Cytokines In Diabetic Patients With Hyperglycemic Crisis. |
PubMed ID 25044235 |
| Liu H, Yang Lx, Guo Rw, Zhu Gf, Shi Yk, Wang Xm, Qi F, Guo Cm, Ye Js, Yang Zh, Liang X. Int J Cardiol. 2013 Oct 9;168(4):3248-53. Doi: 10.1016/J.Ijcard.2013.04.141. Epub 2013 May 1. Functional Blockage Of Emmprin Ameliorates Atherosclerosis In Apo... |
PubMed ID 23642811 |
| Lin Y, Liu Hl, Fang J, Yu Ch, Xiong Yk, Yuan K. Food Chem Toxicol. 2014 Jun;68:290-6. Doi: 10.1016/J.Fct.2014.03.026. Epub 2014 Mar 29. Anti-Fatigue And Vasoprotective Effects Of Quercetin-3-O-Gentiobiose On Oxidative Stress And Vascular Endotheli... |
PubMed ID 24685824 |
| Duan F, Liao J, Huang Q, Nie Y, Wu K. Clin Dev Immunol. 2012;2012:192791. Doi: 10.1155/2012/192791. Epub 2012 Apr 9. Hsv-1 Mir-H6 Inhibits Hsv-1 Replication And Il-6 Expression In Human Corneal Epithelial Cells In Vitro. |
PubMed ID 22550533 |
| Pan Zy, Wang H. Int Immunopharmacol. 2014 May;20(1):229-37. Doi: 10.1016/J.Intimp.2014.03.004. Epub 2014 Mar 19. Synergistic Interaction Between Choline And Aspirin Against Acute Inflammation Induced By Carrageenan And Lipopolysaccharide. |
PubMed ID 24656779 |
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0389 Human IL-1 Alpha/IL-1F1/IL1A ELISA Kit PicoKine™
12 Related Questions
Question
Dear Technical Support: I purchased Human IL-1 Alpha ELISA Kit PicoKine and would like to ask what the nm value to which the absorbance takes place is?
Verified Customer
Asked: 2020-04-29
Answer
The Human IL-1 Alpha ELISA Kit PicoKine states that absorbance of each well on a spectrophotometer using 450 nm as the primary wavelength and optionally 620 nm (610 nm to 650 nm is acceptable) as the reference wavelength.
Boster Scientific Support
Answered: 2020-04-29
Question
Q: is it okay to use citrate plasma as samples in Human IL-1 alpha Picokine® ELISA Kit (Catalog # EK0389)?
Verified Customer
Asked: 2020-10-07
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IL-1 alpha causing disruption of its protein structure. IL-1 alpha may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IL-1 alpha ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-10-07
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
N. Huang
Asked: 2020-10-03
Boster Scientific Support
Answered: 2020-10-03
Question
Q: Can IL-1 alpha ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
R. Turner
Asked: 2020-06-04
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-06-04
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Asked: 2019-12-23
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-12-23
Question
Q: how to thaw whole blood sample for IL-1 alpha ELISA after freezing?
A. Gandhi
Asked: 2019-03-29
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-1 alpha for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-03-29
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of IL-1 alpha in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-1 alpha ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-11-01
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Asked: 2018-10-11
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-10-11
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement IL-1 alpha using the EK0389 Human IL-1 alpha Picokine® ELISA Kit?
J. Mehta
Asked: 2018-04-17
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2018-04-17
Question
Q: if the enzyme conjugated IL-1 alpha antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-1 alpha antigen?
Verified Customer
Asked: 2016-12-07
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-12-07
Question
Q: how do I analyze ELISA data? I have performed IL-1 alpha level in plasma.
H. Jones
Asked: 2016-03-05
Boster Scientific Support
Answered: 2016-03-05
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
P. Kapoor
Asked: 2015-09-20
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2015-09-20
