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Human IL-1 Alpha ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-1 alpha. 96wells/kit, with removable strips. Cited in 9 publication(s).
Human IL-1 Alpha ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0389 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Human |
| Sample Type: | cell culture supernates, serum and plasma( heparin, EDTA, citrate). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <0.5pg/ml |
| Assay Range: | 4.7pg/ml-300pg/ml |
Overview
| Product Name | Human IL-1 Alpha ELISA Kit PicoKine™
See all IL1A primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0389 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Human IL1A with <0.5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma( heparin, EDTA, citrate). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IL1A tissue specificity: |
| Cite This Product | Human IL-1 Alpha ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0389) |
Product Specs
| Reactivity/Species | Human |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, serum and plasma( heparin, EDTA, citrate). |
| Sensitivity | <0.5pg/ml |
| Assay Range | 4.7pg/ml-300pg/ml |
| Immunogen | Expression system for standard: E.coli, Immunogen sequence: S113-A271 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P01583 |
|---|---|
| Gene Name | IL1A |
| Protein Name | Interleukin-1 alpha |
| Subcellular Localization | Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Human IL-1 Alpha ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0389-CAP | 96-well plate precoated with anti-Human IL1A antibody | 1 |
| EK0389-ST | lyophilized recombinant Human IL1A standard | 10ng/tube |
| EK0389-DA | biotinylated anti-Human IL1A antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0389 Human IL-1 Alpha ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 4.7 | 9.4 | 18.8 | 37.5 | 75 | 150 | 300 |
| O.D. | 0.041 | 0.097 | 0.162 | 0.367 | 0.582 | 0.866 | 1.335 | 2.133 |
We measured random samples of Human IL-1 Alpha ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 14 | 52 | 99 | 15 | 49 | 103 |
| Standard deviation | 0.98 | 2.49 | 4.35 | 1.06 | 2.4 | 6.07 |
| CV(%) | 7% | 4.8% | 4.4% | 7.1% | 4.9% | 5.9% |
We measured the performance consistency of Human IL-1 Alpha ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 14 | 15 | 15 | 13 | 14 | 0.82 | 5.8% |
| Sample 2 | 52 | 48 | 50 | 48 | 49 | 1.65 | 3.3% |
| Sample 3 | 99 | 98 | 112 | 102 | 102 | 5.53 | 5.4% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 9
| Pan Zy, Wang H. Int Immunopharmacol. 2014 May;20(1):229-37. Doi: 10.1016/J.Intimp.2014.03.004. Epub 2014 Mar 19. Synergistic Interaction Between Choline And Aspirin Against Acute Inflammation Induced By Carrageenan And Lipopolysaccharide. | PubMed ID 24656779 |
| Duan F, Liao J, Huang Q, Nie Y, Wu K. Clin Dev Immunol. 2012;2012:192791. Doi: 10.1155/2012/192791. Epub 2012 Apr 9. Hsv-1 Mir-H6 Inhibits Hsv-1 Replication And Il-6 Expression In Human Corneal Epithelial Cells In Vitro. | PubMed ID 22550533 |
| Lin Y, Liu Hl, Fang J, Yu Ch, Xiong Yk, Yuan K. Food Chem Toxicol. 2014 Jun;68:290-6. Doi: 10.1016/J.Fct.2014.03.026. Epub 2014 Mar 29. Anti-Fatigue And Vasoprotective Effects Of Quercetin-3-O-Gentiobiose On Oxidative Stress And Vascular Endotheli... | PubMed ID 24685824 |
| Liu H, Yang Lx, Guo Rw, Zhu Gf, Shi Yk, Wang Xm, Qi F, Guo Cm, Ye Js, Yang Zh, Liang X. Int J Cardiol. 2013 Oct 9;168(4):3248-53. Doi: 10.1016/J.Ijcard.2013.04.141. Epub 2013 May 1. Functional Blockage Of Emmprin Ameliorates Atherosclerosis In Apo... | PubMed ID 23642811 |
| Li J, Huang M, Shen X. J Diabetes Complications. 2014 Sep-Oct;28(5):662-6. Doi: 10.1016/J.Jdiacomp.2014.06.008. Epub 2014 Jun 19. The Association Of Oxidative Stress And Pro-Inflammatory Cytokines In Diabetic Patients With Hyperglycemic Crisis. | PubMed ID 25044235 |
| Wang G, Ji Y, Li Z, Han X, Guo N, Song Q, Quan L, Wang T, Han W, Pang D, Ouyang H, Tang X. Sci Rep. 2014 May 9;4:4905. Doi: 10.1038/Srep04905. Nitro-Oleic Acid Downregulates Lipoprotein-Associated Phospholipase A2 Expression Via The P42/P44 Mapk A... | PubMed ID 24809325 |
| Han X, Wang T, Zhang J, Liu X, Li Z, Wang G, Song Q, Pang D, Ouyang H, Tang X. Biol Open. 2015 Apr 2;4(5):661-5. Doi: 10.1242/Bio.201410900. Apolipoprotein Ciii Regulates Lipoprotein-Associated Phospholipase A2 Expression Via The Mapk And Nf??b Pa... | PubMed ID 25836672 |
| Sun X, Shi B, Zheng H, Min L, Yang J, Li X, Liao X, Huang W, Zhang M, Xu S, Zhu Z, Cui H, Liu X. Cell Death Dis. 2018 Feb 15;9(3):260. doi: 10.1038/s41419-018-0303-9. Senescence-associated secretory factors induced by cisplatin in melanoma cells p... | PubMed ID 29449532 |
| Effect of etomidate on the oxidative stress response and levels of inflammatory factors from ischemia-reperfusion injury after tibial fracture surgery | PubMed ID 28450928 |
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Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
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| (888) 466-3604 |
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