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Product Info Summary
| SKU: | A03016 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IL-15R alpha Antibody
SKU/Catalog Number
A03016
Size
100μl
Form
Liquid
Description
Boster Bio Anti-IL-15R alpha Antibody catalog # A03016. Tested in WB, ELISA applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL-15R alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03016)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human IL15RA. AA range:99-148
Reactive Species
A03016 is reactive to IL15RA in Human, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
28.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03016 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot analysis of COLO cells using IL-15Rα Polyclonal Antibody
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Western blot analysis of lysates from COLO cells, using IL15RA Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HeLa cells using IL15RA antibody.
Specific Publications For Anti-IL-15R alpha Antibody (A03016)
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4 Customer Q&As for Anti-IL-15R alpha Antibody
Question
Is a blocking peptide available for product anti-IL-15R alpha antibody (A03016)?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
We do provide the blocking peptide for product anti-IL-15R alpha antibody (A03016). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-18
Question
Would anti-IL-15R alpha antibody A03016 work on feline WB with trachea?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
Our lab technicians have not validated anti-IL-15R alpha antibody A03016 on feline. You can run a BLAST between feline and the immunogen sequence of anti-IL-15R alpha antibody A03016 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline trachea in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-31
Question
Would A03016 anti-IL-15R alpha antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Bhatt
Verified customer
Asked: 2019-04-03
Answer
As indicated on the product datasheet, A03016 anti-IL-15R alpha antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-03
Question
Does anti-IL-15R alpha antibody A03016 work for WB with trachea?
Verified Customer
Verified customer
Asked: 2017-10-03
Answer
According to the expression profile of trachea, IL15RA is highly expressed in trachea. So, it is likely that anti-IL-15R alpha antibody A03016 will work for WB with trachea.
Boster Scientific Support
Answered: 2017-10-03
