Anti-IL7 Antibody Picoband®

IL7 antibody

Boster Bio Anti-IL7 Antibody Picoband® catalog # PB9521. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9521
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-IL7 Antibody Picoband®

View all IL7 Antibodies

SKU/Catalog Number

PB9521
PB0519 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IL7 Antibody Picoband® catalog # PB9521. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IL7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9521)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human IL7 recombinant protein (Position: D26-H177). Human IL7 shares 56.4% and 57% amino acid (aa) sequence identity with mouse and rat IL7, respectively.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9521 is reactive to IL7 in Human

Observed Molecular Weight

75 kDa

Calculated molecular weight

20.2 kDa

Background of IL7

IL-7, Interleukin 7, is a protein that in humans is encoded by the IL-7 gene. IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells but is not produced by lymphocytes. By the combination of approaches, IL-7 is located on 8q12-q13. IL-7 is critical for early T-cell development and homeostasis of naive and memory CD8-positive T cells. It signals through the IL-7 receptor complex, which consists of the IL7R-alpha chain and the common IL2R-gamma chain is critical for early T-cell development and homeostasis of naive and memory CD8 -positive T cells. It signals through the IL-7 receptor complex, which consists of the IL7R-alpha chain and the common IL2R-gamma chain.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9521 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, -
ELISA, 0.1-0.5μg/ml, -

Positive Control

WB: K562 Whole Cell

Validation Images & Assay Conditions

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5 Customer Q&As for Anti-IL7 Antibody Picoband®

Question

We were satisfied with the WB result of your anti-IL7 antibody. However we have seen positive staining in pancreas secreted. using this antibody. Is that expected? Could you tell me where is IL7 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

From what I have seen in literature, pancreas does express IL7. Generally IL7 expresses in secreted. Regarding which tissues have IL7 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-05-01

Question

My lab would like using your anti-IL7 antibody for positive regulation of cell population proliferation studies. Has this antibody been tested with western blotting on k562 whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

Thank you for your inquiry. This PB9521 anti-IL7 antibody is tested on k562 whole cell lysate. It is guaranteed to work for ELISA, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-13

Question

We have been able to see staining in human pancreas. Do you have any suggestions? Is anti-IL7 antibody supposed to stain pancreas positively?

Verified Customer

Verified customer

Asked: 2020-01-27

Answer

From literature pancreas does express IL7. From Uniprot.org, IL7 is expressed in sperm, pancreas, among other tissues. Regarding which tissues have IL7 expression, here are a few articles citing expression in various tissues:
Pancreas, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-01-27

Question

We purchased anti-IL7 antibody for WB on sperm a few months ago. I am using human, and We intend to use the antibody for ELISA next. I would like examining sperm as well as pancreas in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2020-01-10

Answer

I viewed the website and datasheets of our anti-IL7 antibody and I see that PB9521 has been tested on human in both WB and ELISA. Thus PB9521 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-01-10

Question

We are currently using anti-IL7 antibody PB9521 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

The anti-IL7 antibody (PB9521) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-17

Order DetailsPrice
PB9521

100μg

$370
PB9521-Fluoro488

100 μg Fluoro488 conjugated (liquid)

$570
PB9521-Fluoro550

100 μg Fluoro550 conjugated (liquid)

$570
PB9521-Fluoro594

100 μg Fluoro594 conjugated (liquid)

$570
PB9521-Fluoro647

100 μg Fluoro647 conjugated (liquid)

$670
PB9521-APC

100 μg APC conjugated (liquid)

$820
PB9521-FITC

100 μg FITC conjugated (liquid)

$570
PB9521-Cy3

100 μg Cy3 conjugated (liquid)

$570
PB9521-Biotin

100 μg Biotin conjugated (liquid)

$570
PB9521-HRP

100 μg HRP conjugated (liquid)

$570
PB9521-PE

100 μg PE conjugated (liquid)

$820
PB9521-carrier-free

Carrier Free

$370
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Free Secondary Antibody

FREE  $95.00
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$40.00  $80.00
$65.00  $130.00
$67.50  $135.00
$75.00  $150.00
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$75.00  $150.00
$55.00  $110.00
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PB9521
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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Boster Guarantee

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Apr 15, 2026