Product Info Summary
| SKU: | PA1728 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Interleukin-3 IL3 Antibody Picoband®
SKU/Catalog Number
PA1728
BA4807 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interleukin-3 IL3 Antibody catalog # PA1728. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-3 IL3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1728)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse IL-3.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1728 is reactive to Il3 in Mouse
Observed Molecular Weight
14 kDa
Calculated molecular weight
18.5 kDa
Background of Il3
Interleukin 3, also known as IL-3, is a protein that in humans is encoded by the IL3 gene. Ot is mapped to 5q31.1. IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1728 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-IL-3 antibody, PA1728, Western blotting
All lanes: Anti IL-3 (PA1728) at 0.5ug/ml
Lane 1: Recombinant Mouse IL-3 Protein 10ng
Lane 2: Recombinant Mouse IL-3 Protein 5ng
Lane 3: Recombinant Mouse IL-3 Protein 2.5ng
Predicted bind size: 14KD
Observed bind size: 14KD
Specific Publications For Anti-Interleukin-3 IL3 Antibody Picoband® (PA1728)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Interleukin-3 IL3 Antibody Picoband®
Question
I was wanting to use your anti-IL3 antibody for WB for mouse right lobe of liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse right lobe of liver identification?
S. Edwards
Verified customer
Asked: 2020-03-10
Answer
It shows on the product datasheet, PA1728 anti-IL3 antibody has been validated for WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse right lobe of liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-10
Question
Does anti-IL3 antibody PA1728 work for WB with right lobe of liver?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
According to the expression profile of right lobe of liver, IL3 is highly expressed in right lobe of liver. So, it is likely that anti-IL3 antibody PA1728 will work for WB with right lobe of liver.
Boster Scientific Support
Answered: 2019-12-11
Question
See attached the WB image, lot number and protocol we used for right lobe of liver using anti-IL3 antibody PA1728. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-02-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-06
Question
Is this PA1728 anti-IL3 antibody reactive to the isotypes of IL3?
R. Bhatt
Verified customer
Asked: 2016-12-28
Answer
The immunogen of PA1728 anti-IL3 antibody is A synthetic peptide corresponding to a sequence in the middle region of mouse IL-3(122-140aa, RDLDDFRKKLRFYMVHLND). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-12-28
Question
Can you help my question with product PA1728, anti-IL3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Brown
Verified customer
Asked: 2014-03-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1728 anti-IL3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-03-07
Question
Do you have a BSA free version of anti-IL3 antibody PA1728 available?
J. Wu
Verified customer
Asked: 2013-12-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL3 antibody PA1728 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-12-10


