Mouse IL-3 ELISA Kit PicoKine®

IL-3 ELISA kit for Mouse

Mouse IL-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il3 in cell culture supernatants and serum. Sensitivity: 1pg/ml. Cited in 7 publication(s).

Product Info Summary

SKU: EK0403
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Mouse IL-3 ELISA Kit PicoKine®

View all IL-3 ELISA kits

SKU/Catalog Number

EK0403

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IL-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il3 in cell culture supernatants and serum. Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse IL-3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0403)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A27-C166

Sensitivity

<1 pg/ml

Assay Range

7.8 pg/ml - 500 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0403 is reactive to Il3 in Mouse samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK0403 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL-3

The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3 (IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5. The standard product used in this kit is recombinant mouse IL-3, consisting of 135 amino acids with the molecular mass of 15KDa.

Kit Components

Catalog Number Description Quantity
EK0403-CAP Anti-Mouse Il3 Pre-coated 96-well strip microplate 1
EK0403-ST Mouse Il3 Standard 2 vials, 10 ng/tube
EK0403-DA Mouse Il3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)07.815.631.362.5125250500
O.D.0.0290.0780.1430.2340.4240.8461.2891.909

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-3, Dilution ratio of 1:1.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IL-3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)161012781598298
Standard deviation1.157.2517.791.145.4824.43
CV (%)7.2%5.2%6.4%7.6%5.6%8.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 116171617160.53.1%
Sample 2101102103941003.533.5%
Sample 327825425723125516.656.5%
*number of samples for each test n=16.

Gene/Protein Information For Il3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P01586

Gene ID

16187

Gene Name

Il3

Full Name

Interleukin-3

Weight

18.54kDa

Superfamily

IL-3 family

Alternative Names

Hematopoietic growth factor; IL3; IL-3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGF; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor Il3|B, BPA, Csfm, Csfmu, HCGF, Il, Il-3, MCGF, P, PSF|interleukin 3|interleukin-3|P-cell-stimulating factor|hematopoietic growth factor|mast cell growth factor|multipotential colony-stimulating factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il3, check out the Il3 Infographic

Il3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0403 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Antiasthmatic Effects of Sanglong Pingchuan Decoction through Inducing a Balanced Th1/Th2 Immune Response

Astrocytic interleukin-3 programs microglia and limits Alzheimer’s disease

Toll-like receptors 2 and 4 mediate the capacity of mesenchymal stromal cells to support the proliferation and differentiation of CD34+ cells

Systemic delivery of TNF-armed myxoma virus plus immune checkpoint inhibitor eliminates lung metastatic mouse osteosarcoma

Self-reactive CD4+ IL-3+ T cells amplify autoimmune inflammation in myocarditis by inciting monocyte chemotaxis
Species: Mouse

Wu Z, Ming Y, Zhang Z, Wu J, Wu Z, Yao S. Chin Med J (Engl). 2014;127(5):968-70. Edaravone Rescues The Lung By Inhibiting Lipid Peroxidation And Pro-Inflammatory Cytokines In A Rat Model.

Guida F, Luongo L, Aviello G, Palazzo E, De Chiaro M, Gatta L, Boccella S, Marabese I, Zjawiony Jk, Capasso R, Izzo Aa, De Novellis V, Maione S. Mol Pain. 2012 Aug 23;8:60. Doi: 10.1186/1744-8069-8-60. Salvinorin A Reduces Mechanical Allodynia And...

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5 Customer Q&As for Mouse IL-3 ELISA Kit PicoKine®

Question

Q: if the enzyme conjugated IL-3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-3 antigen?

Verified Customer

Verified customer

Asked: 2020-09-30

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-09-30

Question

Q: how to thaw whole blood sample for IL-3 ELISA after freezing?

S. Anand

Verified customer

Asked: 2019-11-25

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-3 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-11-25

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

B. Jha

Verified customer

Asked: 2018-12-17

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-12-17

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-11-08

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-11-08

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2014-03-27

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2014-03-27

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