Anti-Interleukin-3 IL3 Antibody Picoband®

Product Name

Anti-Interleukin-3 IL3 Antibody Picoband®

View all Il3 Antibodies

SKU/Catalog Number

RP1046
PB0246 is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Interleukin-3 IL3 Antibody catalog # RP1046. Tested in ELISA, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Interleukin-3 IL3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1046)

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived rat IL-3 recombinant protein (Position: S28-C166). Rat IL-3 shares 32% and 55% amino acid (aa) sequence identity with human and mouse IL-3, respectively.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1046 is reactive to Il3 in Rat

Observed Molecular Weight

37 kDa

Calculated molecular weight

18.6 kDa

Background of Il3

Interleukin 3, also known as IL-3, is a protein that in humans is encoded by the IL3 gene. It is mapped to 5q31.1. IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

RP1046 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA, 0.1-0.5μg/ml, -
Western blot, 0.1-0.5μg/ml, Rat

Positive Control

WB: PC-12 Whole Cell, NRK Whole Cell, RH35 Whole Cell

Validation Images & Assay Conditions

See full target information Il3

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