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- Table of Contents
IL32 antibody (A03286-2) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence Validation of IL-32 in Human Spleen Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen cells labeling IL-32 with A03286-2 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen cells labeling IL-32 with A03286-2 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Anti-IL-32 Antibody
IL32 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
WB: 0.5-4 μg/mL; IHC: 2-10 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-IL-32 Antibody
View all IL32 Antibodies
View all IL32 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
IL-32 antibody can be stored at 4°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
IL-32 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-IL-32 antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human IL-32. The immunogen is located within the last 50 amino acids of IL-32.
Cross-Reactivity
This antibody detects all isoforms of IL-32.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
26.7 kDa
Background of IL32
Interleukin-32 (IL-32) was initially identified as a transcript (NK4) that is selectively expressed in lymphocytes and NK cells and whose expression is increased following activation by IL-2. It was later re-isolated from an IL-18-treated lung carcinoma cell line and re-named IL-32. IL-32 is unusual in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four differentially spliced isoforms. Because treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-α, IL-8 and MIP-2, and because it is also induced in human peripheral lymphocyte cells after mitogen stimulation and in epithelial cells by IFNγ, it has been suggested that IL-32 may play a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.
Cite This Product
Anti-IL-32 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03286-2)
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Product Promise
We promise Anti-IL-32 Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A03286-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 21, 2026