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Product Info Summary
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Anti-IL-32A Antibody Peroxidase Conjugated
Boster Bio Anti-IL-32A Antibody Peroxidase Conjugated catalog # A03286-1. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Cite This Product
Anti-IL-32A Antibody Peroxidase Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03286-1)
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-32A protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
A03286-1 is reactive to IL32 in Human
A03286-1 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western Blot: 1:1,000 - 1:5,000
Immunohistochemistry: 1:500 - 1:2,500
Other Dilution: User Optimized
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL32 using anti-IL32 antibody (A03286-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL32 antigen affinity purified polyclonal antibody (Catalog # A03286-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for IL32.
Gene/Protein Information For IL32 (Source: Uniprot.Org, NCBI)
IL32; IL-32; IL-32alpha; IL-32beta; interleukin 32; interleukin-32 theta; interleukin-32; natural killer cell transcript 4; Natural killer cells protein 4; NK4; NK4IL-32delta; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; TAIFIL-32gamma; Tumor necrosis factor alpha-inducing factor IL32 IL-32alpha, IL-32beta, IL-32delta, IL-32gamma, NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd interleukin 32 interleukin-32|interleukin-32 eta|interleukin-32 small|interleukin-32 theta|natural killer cell transcript 4|natural killer cells protein 4|tumor necrosis factor alpha-inducing factor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IL32, check out the IL32 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL32: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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