Anti-IL-32A Antibody Peroxidase Conjugated

Product Name

Anti-IL-32A Antibody Peroxidase Conjugated

View all IL32 Antibodies

SKU/Catalog Number

A03286-1

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-IL-32A Antibody Peroxidase Conjugated (Catalog # A03286-1). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)

Cite This Product

Anti-IL-32A Antibody Peroxidase Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03286-1)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, antibody formulated with stabilizing protein (10 mg/mL) - Immunoglobulin and Protease free, 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

IgG

Immunogen

This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-32A protein.

Reactive Species

A03286-1 is reactive to IL32 in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

26.7 kDa

Background of IL32

IL-32A (also known as Natural killer cells protein 4, Tumor necrosis factor alpha-inducing factor, IL32α, Interleukin-32 and IL-32 isoform 4) is a member of the cytokine family. IL-32a is a secreted protein selectively expressed in lymphocytes and plays a role in innate and adaptive immune responses. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFα and IL-8. It induces typical cytokine pathways of NF-kB and p38 MAPK. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03286-1 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IHC: 1:500 - 1:2,500
WB: 1:1,000 - 1:5,000
This purified antibody has been tested in western blotting and suitable for ELISA. By western blot a band approximately 15 kDa in size corresponding to native human IL-32α protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Validation Images & Assay Conditions

See full target information IL32

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