Anti-Il13 Antibody Picoband®

Western blot analysis of Il13 using anti-Il13 antibody (A00077-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Il13 antigen affinity purified polyclonal antibody (Catalog # A00077-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Il13 at approximately 19 kDa. The expected band size for Il13 is at 19 kDa.

RCM-1 prevents type 2 cytokines expression in mice to expel H . nana . A Representative images of IHC with GATA3 (brown highlighted by black arrowheads, scale bars 50 μm). B Representative images of IHC with IL-13 (brown highlighted by red arrowheads, scale bars 100 μm for the upper panel and 50 μm for the lower panel). Percentages of positive-area of GATA3 ( C ) and IL-13 ( D ) were semi-quantified using Image J software. E The protein level of IL-4 (left panel) and the percentage of the relative expression of IL-4 were semi-quantified using Image J software (right panel). The relative quantifications of transcription levels of IL-13 ( F ), IL-4 ( G ), IL-5 ( H ), and IL-9 ( I ) were determined by the 2 −ΔΔCt method normalized to GAPDH . Data are presented as mean + SD for ( C – I ) and the right panel of ( E ), n = 7 per group, ** P < 0.01, *** P < 0.001; ns, not statistically significant
Index in PubMed under a CC BY license. PMID: 40069907

ESPs increase the number of tuft cells and IL-13 in the small intestine of UC mice. A Representative images of IHC for Dclk1 (brown indicated by red arrowheads in the lower panel; scale bars 100 μm for the upper panel and 50 μm for the lower panel) and ( B ) IF for Dclk1 (green) and the nucleus (4′,6-diamidino-2-phenylindole (DAPI), blue) (scale bars 100 μm). Percentages of Dclk1-positive area and the number of tuft cells were semi-quantified using Image J in A and B . The transcription levels of Dclk1 ( C ), IL-25 ( D ), IL-33 ( E ), and IL-13 ( F ) were determined using the 2 −ΔΔCt method normalized to GAPDH . G Representative images of IHC for IL-13 (brown indicated by black arrowheads in the lower panel; scale bars 100 μm for the upper panel and 50 μm for the lower panel); percentage of IL-13-positive area was semi-quantified using Image J. Data are presented as mean + SD for the right panel of A , B , G ) and ( C – F ). Group size of n = 7 per group. * p < 0.05; ** p < 0.01; *** p < 0.001 Full size image
Index in PubMed under a CC BY license. PMID: 40542404

ESPs enhance ISC proliferation and differentiation to promote intestinal epithelial regeneration and alleviate organoid damage. ( A ) WB results for Lgr5 and PCNA (left panel) and the relative expression levels of Lgr5 and PCNA (right panel) were semi-quantified using Image J. ( B ) WB results for Dclk1, Lyz, and MUC2 (left panel); the relative expression levels of Dclk1, Lyz, and MUC2 were semi-quantified using Image J. The transcription levels of Lgr5 ( C ), Dclk1 ( D ), Lyz1 ( E ), MUC2 ( F ), and IL-13 ( H ) were determined using the 2 −ΔΔCt method normalized to GAPDH . ( G ) WB result for IL-13 (left panel) and the relative expression level of IL-13 was semi-quantified using Image J. Data are presented as mean + SD for the right panels of A , B & G and C – F and H . Group size of n = 3 per group. * p < 0.05; ** p < 0.01; *** p < 0.001 Full size image
Index in PubMed under a CC BY license. PMID: 40542404

IHC analysis of Il13 using anti-Il13 antibody (A00077-2).
Il13 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Il13 Antibody (A00077-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

IHC analysis of Il13 using anti-Il13 antibody (A00077-2).
Il13 was detected in a paraffin-embedded section of rat lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Il13 Antibody (A00077-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.