Anti-Interleukin-13 IL13 Antibody

IL-13 antibody

Boster Bio Anti-Interleukin-13 IL13 Antibody catalog # RP1047. Tested in ELISA, WB applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: RP1047
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-Interleukin-13 IL13 Antibody

View all IL-13 Antibodies

SKU/Catalog Number

RP1047

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Interleukin-13 IL13 Antibody catalog # RP1047. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Interleukin-13 IL13 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1047)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human IL-13 recombinant protein (Position: L25-N146). Human IL-13 shares 57% and 62% amino acid (aa) sequence identity with mouse and rat IL-13, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1047 is reactive to IL13 in Human

Applications

RP1047 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

37 kDa

Calculated molecular weight

15.816kDa

Background of IL-13

IL-13, Interleukin 13, is a protein that in humans is encoded by the IL-13 gene. IL-13 is a kind of cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, which is an important mediator of allergic inflammation and disease. The IL-13 gene is mapped to 5q23-q31. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains. This gene acts more prominently as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thereby altering physiological function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For IL13 (Source: Uniprot.org, NCBI)

Gene Name

IL13

Full Name

Interleukin-13

Weight

15.816kDa

Superfamily

IL-4/IL-13 family

Alternative Names

ALRHMGC116789; BHR1interleukin-13; IL13; IL-13; IL-13MGC116788; interleukin 13; MGC116786; NC30; P600 IL13 IL-13, P600 interleukin 13 interleukin-13

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IL13, check out the IL13 Infographic

IL13 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IL13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

RP1047 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

CD68 and interleukin 13, prospective immune markers for esophageal squamous cell carcinoma prognosis prediction

Li J, Li L, Zuo H, Ke C, Yan B, Wen H, Zhang Y, Yang X. Plos One. 2014 Feb 3;9(2):E87887. Doi: 10.1371/Journal.Pone.0087887. Ecollection 2014. T-Helper Type-2 Contact Hypersensitivity Of Balb/C Mice Aggravated By Dibutyl Phthalate Via Long-Term De...

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7 Customer Q&As for Anti-Interleukin-13 IL13 Antibody

Question

Is this RP1047 anti-IL13 antibody reactive to the isotypes of IL13?

Verified Customer

Verified customer

Asked: 2020-02-21

Answer

The immunogen of RP1047 anti-IL13 antibody is E. coli-derived human IL-13 recombinant protein (Position: L25-N146). Human IL-13 shares 57% and 62% amino acid (aa) sequence identity with mouse and rat IL-13, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-21

Question

We purchased anti-IL13 antibody for WB on blood in a previous experiment. I am using human, and I plan to use the antibody for ELISA next. Our lab want to know about examining blood as well as left testis in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2019-11-06

Answer

I took a look at the website and datasheets of our anti-IL13 antibody and it seems that RP1047 has been validated on human in both WB and ELISA. Thus RP1047 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-11-06

Question

We have observed staining in human left testis. Any tips? Is anti-IL13 antibody supposed to stain left testis positively?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

According to literature left testis does express IL13. According to Uniprot.org, IL13 is expressed in left testis, blood, peripheral blood lymphocyte, among other tissues. Regarding which tissues have IL13 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7721105
Peripheral blood lymphocyte, Pubmed ID: 1408833

Boster Scientific Support

Answered: 2019-08-26

Question

Our lab were well pleased with the WB result of your anti-IL13 antibody. However we have observed positive staining in peripheral blood lymphocyte secreted. using this antibody. Is that expected? Could you tell me where is IL13 supposed to be expressed?

M. Singh

Verified customer

Asked: 2019-08-20

Answer

From what I have seen in literature, peripheral blood lymphocyte does express IL13. Generally IL13 expresses in secreted. Regarding which tissues have IL13 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7721105
Peripheral blood lymphocyte, Pubmed ID: 1408833

Boster Scientific Support

Answered: 2019-08-20

Question

We are currently using anti-IL13 antibody RP1047 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2017-12-01

Answer

The anti-IL13 antibody (RP1047) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-12-01

Question

I was wanting to use your anti-IL13 antibody for ELISA for human peripheral blood lymphocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human peripheral blood lymphocyte identification?

K. Collins

Verified customer

Asked: 2015-07-07

Answer

You can see on the product datasheet, RP1047 anti-IL13 antibody has been validated for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human peripheral blood lymphocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-07-07

Question

you antibody to test anti-IL13 antibody RP1047 on human peripheral blood lymphocyte for research purposes, then I may be interested in using anti-IL13 antibody RP1047 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

A. Anderson

Verified customer

Asked: 2014-04-22

Answer

The products we sell, including anti-IL13 antibody RP1047, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-04-22

Order DetailsPrice
RP1047

100μg

$370
RP1047-10ug

10μg sample (liquid)

$99
RP1047-Biotin

100 μg Biotin conjugated

$570
RP1047-Cy3

100 μg Cy3 conjugated

$570
RP1047-Dylight488

100 μg Dylight488 conjugated

$570
RP1047-Dylight550

100 μg Dylight550 conjugated

$570
RP1047-Dylight594

100 μg Dylight594 conjugated

$570
RP1047-FITC

100 μg FITC conjugated

$570
RP1047-HRP

100 μg HRP conjugated

$570
RP1047-APC

100 μg APC conjugated

$670
RP1047-PE

100 μg PE conjugated

$670
RP1047-carrier-free

Carrier Free

$370

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RP1047
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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