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Product Info Summary
| SKU: | RP1047 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Interleukin-13 IL13 Antibody Picoband®
SKU/Catalog Number
RP1047
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Interleukin-13 IL13 Antibody catalog # RP1047. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Interleukin-13 IL13 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1047)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human IL-13 recombinant protein (Position: L25-N146). Human IL-13 shares 57% and 62% amino acid (aa) sequence identity with mouse and rat IL-13, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1047 is reactive to IL13 in Human
Observed Molecular Weight
37 kDa
Calculated molecular weight
15.8 kDa
Background of IL13
IL-13, Interleukin 13, is a protein that in humans is encoded by the IL-13 gene. IL-13 is a kind of cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, which is an important mediator of allergic inflammation and disease. The IL-13 gene is mapped to 5q23-q31. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains. This gene acts more prominently as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thereby altering physiological function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1047 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Validation Images & Assay Conditions
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Boster Kit Box
Specific Publications For Anti-Interleukin-13 IL13 Antibody Picoband® (RP1047)
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Customer Q&As
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7 Customer Q&As for Anti-Interleukin-13 IL13 Antibody Picoband®
Question
Is this RP1047 anti-IL13 antibody reactive to the isotypes of IL13?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
The immunogen of RP1047 anti-IL13 antibody is E. coli-derived human IL-13 recombinant protein (Position: L25-N146). Human IL-13 shares 57% and 62% amino acid (aa) sequence identity with mouse and rat IL-13, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-21
Question
We purchased anti-IL13 antibody for WB on blood in a previous experiment. I am using human, and I plan to use the antibody for ELISA next. Our lab want to know about examining blood as well as left testis in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
I took a look at the website and datasheets of our anti-IL13 antibody and it seems that RP1047 has been validated on human in both WB and ELISA. Thus RP1047 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-06
Question
We have observed staining in human left testis. Any tips? Is anti-IL13 antibody supposed to stain left testis positively?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
According to literature left testis does express IL13. According to Uniprot.org, IL13 is expressed in left testis, blood, peripheral blood lymphocyte, among other tissues. Regarding which tissues have IL13 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7721105
Peripheral blood lymphocyte, Pubmed ID: 1408833
Boster Scientific Support
Answered: 2019-08-26
Question
Our lab were well pleased with the WB result of your anti-IL13 antibody. However we have observed positive staining in peripheral blood lymphocyte secreted. using this antibody. Is that expected? Could you tell me where is IL13 supposed to be expressed?
M. Singh
Verified customer
Asked: 2019-08-20
Answer
From what I have seen in literature, peripheral blood lymphocyte does express IL13. Generally IL13 expresses in secreted. Regarding which tissues have IL13 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7721105
Peripheral blood lymphocyte, Pubmed ID: 1408833
Boster Scientific Support
Answered: 2019-08-20
Question
We are currently using anti-IL13 antibody RP1047 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
The anti-IL13 antibody (RP1047) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-01
Question
I was wanting to use your anti-IL13 antibody for ELISA for human peripheral blood lymphocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human peripheral blood lymphocyte identification?
K. Collins
Verified customer
Asked: 2015-07-07
Answer
You can see on the product datasheet, RP1047 anti-IL13 antibody has been validated for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human peripheral blood lymphocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-07-07
Question
you antibody to test anti-IL13 antibody RP1047 on human peripheral blood lymphocyte for research purposes, then I may be interested in using anti-IL13 antibody RP1047 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Anderson
Verified customer
Asked: 2014-04-22
Answer
The products we sell, including anti-IL13 antibody RP1047, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-04-22
