Anti-IPO-38 HMGN1 Monoclonal Antibody

Product Name

Anti-IPO-38 HMGN1 Monoclonal Antibody

View all HMGN1 Antibodies

SKU/Catalog Number

M03051

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-IPO-38 HMGN1 Monoclonal Antibody (Catalog # M03051). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-IPO-38 HMGN1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03051)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: IPO-38 IgM kappa

Isotype

IgM kappa

Immunogen

A BALB/c mouse was immunized with spleen cells of a human patient with hairy cell leukemia.

Reactive Species

M03051 is reactive to HMGN1 in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

10.2 kDa

Background of HMGN1

The specificity of mAb IPO-38 was demonstrated in different human and murine lymphoid and non-lymphoid cell lines. It does not react with mononuclear cells and granulocytes of healthy donors but does react with nuclei of blood cells of patients with different forms of leukemia and with cells from lymph nodes of patients with Hodgkin's disease and non-hodgkin's lymphoma. Also a positive reaction in nuclei of breast and colorectal tumor cells was demonstrated. IPO-38 may be a good marker of cellular proliferation during monitoring of tumor progression.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03051 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IHC: 10 µg/mL
WB: 1:500- 1:2,000
Anti-IPO-38 has been tested by immunohistochemistry (frozen and formalin/paraffin) and western blot analysis on the Raji cell line and reacts with a 14 -16 kDa protein. This antibody is suitable for ELISA and immunoprecipitation.  The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells (paraformaldehyde fixed, Triton X-100 permeabilized).  An increase of 400% is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemid).  IPO-38 does not block the binding of the Ki-67 antibody.   Tonsillar tissue is typically used as a positive control.

Validation Images & Assay Conditions

See full target information HMGN1

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