Product Info Summary
SKU: | A00027Y570 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-JAK2 (phospho-Y570) Antibody
SKU/Catalog Number
A00027Y570
Size
100ul
Form
Liquid
Description
Boster Bio Anti-JAK2 (phospho-Y570) Antibody catalog # A00027Y570. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-JAK2 (phospho-Y570) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00027Y570)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Clone Number
PI9-17
Isotype
IgG
Immunogen
Synthesized peptide derived from human JAK2 around the phosphorylation site of Y221.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Weakly cross-reacts with dog p53.
Reactive Species
A00027Y570 is reactive to JAK2 in Human, Mouse, Rat
Applications
A00027Y570 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
130.674kDa
Background of JAK2
Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.
Saltzman A., Biochem. Biophys. Res. Commun. 246:627-633(1998).
Dalal I., Blood 91:844-851(1998).
Peeters P., Blood 90:2535-2540(1997)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blotting validation for Anti-JAK2 (phospho-Y570) Antibody A00027Y570
Western blot (WB) analysis of p-JAK2 (Y570) polyclonal antibody at 1:500 dilutionLane1:Hela cell lysate
Lane2:NIH-3T3 cell lysate
Lane3:Rat kidney tissue lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For JAK2 (Source: Uniprot.org, NCBI)
Gene Name
JAK2
Full Name
Tyrosine-protein kinase JAK2
Weight
130.674kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.10; EC 2.7.10.2; Jak2; JAK-2; Janus kinase 2Janus kinase 2 (a protein tyrosine kinase); JTK10; tyrosine-protein kinase JAK2 JAK2 JTK10 Janus kinase 2 tyrosine-protein kinase JAK2|JAK-2|Janus kinase 2 (a protein tyrosine kinase)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on JAK2, check out the JAK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for JAK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-JAK2 (phospho-Y570) Antibody (A00027Y570)
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No publications found for A00027Y570
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