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Product Info Summary
| SKU: | M09979 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-KCTD5 Mouse Monoclonal Antibody [Clone ID: OTI3C8]
SKU/Catalog Number
M09979
Size
100 µl
Description
Boster Bio Anti-KCTD5 mouse monoclonal antibody, clone OTI3C8 (formerly 3C8). Catalog# M09979. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-KCTD5 Mouse Monoclonal Antibody [Clone ID: OTI3C8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09979)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3C8
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human KCTD5 (NP_061865) produced in HEK293T cell.
Reactive Species
M09979 is reactive to KCTD5 in Human, Mouse, Rat
Calculated molecular weight
26.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09979 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100~400
WB: 1:200
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KCTD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KCTD5.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KCTD5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-KCTD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-KCTD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-KCTD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either pCMV6-ENTRY KCTD5 (Red) or empty vector control plasmid (Blue) were immunostained with anti-KCTD5 mouse monoclonal (M09979)
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Anti-KCTD5 mouse monoclonal antibody (M09979) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KCTD5.
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Immunofluorescent staining of HeLa cells using anti-KCTD5 mouse monoclonal antibody (M09979).
Specific Publications For Anti-KCTD5 Mouse Monoclonal Antibody [Clone ID: OTI3C8] (M09979)
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