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pb9071 oncotarget 07 8043 g002 Lentivirus-mediated PLCγ1 shRNA could suppress migration in human gastric adenocarcinoma BGC-823 cells. Cells were transduced with lentivirus-mediated PLCγ1 shRNA2/3 vectors. ( A ) The formation of membrane ruffles was detected using Ruffling assay as described in Materials and Methods. The cell nuclei were stained DAPI (blue) and the membrane ruffles were stained rhodamine-conjugated phalloidin (red). Scale bar = 10 μm. ( B and C ) The migration ability was measured using Transwell assay (B, magnification × 100) and Scratch assay (C, magnification × 400) as described in Materials and Methods. ( D ) The protein levels of MMP2, MMP9, E-cadherin, N-cadherin, snail, slug, and GAPDH were detected with Western blotting analysis, and the pro and active forms of MMP2/9 were observed using gelatin zymography assay as described in Materials and Methods. ( E ) The mRNA levels of PLCG1, MMP2, MMP9, CDHI, CDH2, SNAIL, SLUG, and GAPDH were detected using Real-time PCR analysis as described in Materials and Methods. ( F ) The level of VEGF in extracellular matrix was detected using ELISA as described in Materials and Methods. Data are reported as means ± S.D. of three independent experiments (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, vs respective control).Index in PubMed under a CC BY license. PMID: 26811493
Lentivirus-mediated PLCγ1 shRNA could suppress migration in human gastric adenocarcinoma BGC-823 cells. Cells were transduced with lentivirus-mediated PLCγ1 shRNA2/3 vectors. ( A ) The formation of membrane ruffles was detected using Ruffling assay as described in Materials and Methods. The cell nuclei were stained DAPI (blue) and the membrane ruff... View Full Description
View all 6 images
pb9071 oncotarget 07 8043 g002 Lentivirus-mediated PLCγ1 shRNA could suppress migration in human gastric adenocarcinoma BGC-823 cells. Cells were transduced with lentivirus-mediated PLCγ1 shRNA2/3 vectors. ( A ) The formation of membrane ruffles was detected using Ruffling assay as described in Materials and Methods. The cell nuclei were stained DAPI (blue) and the membrane ruffles were stained rhodamine-conjugated phalloidin (red). Scale bar = 10 μm. ( B and C ) The migration ability was measured using Transwell assay (B, magnification × 100) and Scratch assay (C, magnification × 400) as described in Materials and Methods. ( D ) The protein levels of MMP2, MMP9, E-cadherin, N-cadherin, snail, slug, and GAPDH were detected with Western blotting analysis, and the pro and active forms of MMP2/9 were observed using gelatin zymography assay as described in Materials and Methods. ( E ) The mRNA levels of PLCG1, MMP2, MMP9, CDHI, CDH2, SNAIL, SLUG, and GAPDH were detected using Real-time PCR analysis as described in Materials and Methods. ( F ) The level of VEGF in extracellular matrix was detected using ELISA as described in Materials and Methods. Data are reported as means ± S.D. of three independent experiments (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, vs respective control).Index in PubMed under a CC BY license. PMID: 26811493pb9071 oncotarget 07 8043 g003 Depletion of PLCγ1 suppresses growth and metastasis of gastric adenocarcinoma in a nude mouse tumor xenograft model. ( A ) Volume and weight of tumor samples from nude mice. ( B ) The protein levels of PCNA, cleaved-PARP, PARP, and Bcl-2 in the tumor samples were detected by Immunohistochemistry (Magnificationx100, x400) and Western blotting analyses as described in Materials and Methods. ( C ) The levels of MMP2 and MMP9 in the tumor samples were detected by Immunohistochemistry analysis as described in Materials and Methods (Magnificationx100, x400). The protein and mRNA levels of MMP2, MMP9, E-cadherin(CDH1), N-cadherin(CDH2), snail(SNAIL), and slug(SLUG) in the tumor samples were detected by Western Blotting and Real-time PCR analyses as described in Materials and Methods. ( D ) The protein levels of VEGF and CD34 and the mRNA level of VEGF in tumor samples were detected by Immunohistochemistry and Real-time PCR analysis as described in Materials and Methods. The number of microvessels was accounted under OLYPUS x41 microscope (Magnification x100, x400). ( E ) The lymphoid follicles in inguinal lymph nodes of nude mice were observed under OLYPUS x41microscope, and the protein levels of CD44 and GFP in inguinal lymph nodes of nude mice was detected by Immunohistochemistry analysis as described in Materials and Methods (Magnificationx40, x400). Data are reported as means ± S.D. of three independent experiments (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, vs respective control).Index in PubMed under a CC BY license. PMID: 26811493pb9071 vegfa primary antibodies wb testing 1 Western blot analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: recombinant human VEGF/VEGFA protein 20ng, Lane 2: recombinant human VEGF/VEGFA protein 10ng, Lane 3: human U87 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF/VEGFA antigen affinity purified polyclonal antibody (PB9071) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for VEGF/VEGFA at approximately 18, 27 kDa. The expected band size for VEGF/VEGFA is at 27 kDa.pb9071 vegfa primary antibodies ihc testing 1 IHC analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071). VEGF/VEGFA was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VEGF/VEGFA Antibody (PB9071) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.pb9071 vegfa primary antibodies ihc testing 2 IHC analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071). VEGF/VEGFA was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VEGF/VEGFA Antibody (PB9071) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Validation Summary

Summary of antibody validation, including methods and results to ensure specificity and affinity.

Tested Applications & Dilutions

List of tested applications (e.g., WB, IHC, ELISA) and recommended dilution ranges.

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Property & Storage

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Target Data (AI Summarized)

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Anti-VEGF/VEGFA Antibody Picoband®

antibody

Boster Bio Anti-VEGF/VEGFA Antibody Picoband® catalog # PB9071. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in publication(s). Independently reviewed in review(s).

pb9071 oncotarget 07 8043 g002 Lentivirus-mediated PLCγ1 shRNA could suppress migration in human gastric adenocarcinoma BGC-823 cells. Cells were transduced with lentivirus-mediated PLCγ1 shRNA2/3 vectors. ( A ) The formation of membrane ruffles was detected using Ruffling assay as described in Materials and Methods. The cell nuclei were stained DAPI (blue) and the membrane ruffles were stained rhodamine-conjugated phalloidin (red). Scale bar = 10 μm. ( B and C ) The migration ability was measured using Transwell assay (B, magnification × 100) and Scratch assay (C, magnification × 400) as described in Materials and Methods. ( D ) The protein levels of MMP2, MMP9, E-cadherin, N-cadherin, snail, slug, and GAPDH were detected with Western blotting analysis, and the pro and active forms of MMP2/9 were observed using gelatin zymography assay as described in Materials and Methods. ( E ) The mRNA levels of PLCG1, MMP2, MMP9, CDHI, CDH2, SNAIL, SLUG, and GAPDH were detected using Real-time PCR analysis as described in Materials and Methods. ( F ) The level of VEGF in extracellular matrix was detected using ELISA as described in Materials and Methods. Data are reported as means ± S.D. of three independent experiments (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, vs respective control).Index in PubMed under a CC BY license. PMID: 26811493
Lentivirus-mediated PLCγ1 shRNA could suppress migration in human gastric adenocarcinoma BGC-823 cells. Cells were transduced with lentivirus-mediated PLCγ1 shRNA2/3 vectors. ( A ) The formation of membrane ruffles was detected using Ruffling assay as described in Materials and Methods. The cell nuclei were stained DAPI (blue) and the membrane ruff... View Full Description
View all 6 images
pb9071 oncotarget 07 8043 g002 Lentivirus-mediated PLCγ1 shRNA could suppress migration in human gastric adenocarcinoma BGC-823 cells. Cells were transduced with lentivirus-mediated PLCγ1 shRNA2/3 vectors. ( A ) The formation of membrane ruffles was detected using Ruffling assay as described in Materials and Methods. The cell nuclei were stained DAPI (blue) and the membrane ruffles were stained rhodamine-conjugated phalloidin (red). Scale bar = 10 μm. ( B and C ) The migration ability was measured using Transwell assay (B, magnification × 100) and Scratch assay (C, magnification × 400) as described in Materials and Methods. ( D ) The protein levels of MMP2, MMP9, E-cadherin, N-cadherin, snail, slug, and GAPDH were detected with Western blotting analysis, and the pro and active forms of MMP2/9 were observed using gelatin zymography assay as described in Materials and Methods. ( E ) The mRNA levels of PLCG1, MMP2, MMP9, CDHI, CDH2, SNAIL, SLUG, and GAPDH were detected using Real-time PCR analysis as described in Materials and Methods. ( F ) The level of VEGF in extracellular matrix was detected using ELISA as described in Materials and Methods. Data are reported as means ± S.D. of three independent experiments (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, vs respective control).Index in PubMed under a CC BY license. PMID: 26811493pb9071 oncotarget 07 8043 g003 Depletion of PLCγ1 suppresses growth and metastasis of gastric adenocarcinoma in a nude mouse tumor xenograft model. ( A ) Volume and weight of tumor samples from nude mice. ( B ) The protein levels of PCNA, cleaved-PARP, PARP, and Bcl-2 in the tumor samples were detected by Immunohistochemistry (Magnificationx100, x400) and Western blotting analyses as described in Materials and Methods. ( C ) The levels of MMP2 and MMP9 in the tumor samples were detected by Immunohistochemistry analysis as described in Materials and Methods (Magnificationx100, x400). The protein and mRNA levels of MMP2, MMP9, E-cadherin(CDH1), N-cadherin(CDH2), snail(SNAIL), and slug(SLUG) in the tumor samples were detected by Western Blotting and Real-time PCR analyses as described in Materials and Methods. ( D ) The protein levels of VEGF and CD34 and the mRNA level of VEGF in tumor samples were detected by Immunohistochemistry and Real-time PCR analysis as described in Materials and Methods. The number of microvessels was accounted under OLYPUS x41 microscope (Magnification x100, x400). ( E ) The lymphoid follicles in inguinal lymph nodes of nude mice were observed under OLYPUS x41microscope, and the protein levels of CD44 and GFP in inguinal lymph nodes of nude mice was detected by Immunohistochemistry analysis as described in Materials and Methods (Magnificationx40, x400). Data are reported as means ± S.D. of three independent experiments (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, vs respective control).Index in PubMed under a CC BY license. PMID: 26811493pb9071 vegfa primary antibodies wb testing 1 Western blot analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: recombinant human VEGF/VEGFA protein 20ng, Lane 2: recombinant human VEGF/VEGFA protein 10ng, Lane 3: human U87 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF/VEGFA antigen affinity purified polyclonal antibody (PB9071) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for VEGF/VEGFA at approximately 18, 27 kDa. The expected band size for VEGF/VEGFA is at 27 kDa.pb9071 vegfa primary antibodies ihc testing 1 IHC analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071). VEGF/VEGFA was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VEGF/VEGFA Antibody (PB9071) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.pb9071 vegfa primary antibodies ihc testing 2 IHC analysis of VEGF/VEGFA using anti-VEGF/VEGFA antibody (PB9071). VEGF/VEGFA was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-VEGF/VEGFA Antibody (PB9071) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Product Info Summary

SKU: PB9071
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, WB

Product Name

Anti-VEGF/VEGFA Antibody Picoband®

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SKU/Catalog Number

PB9071
is an alternative SKU for this antibody, used in previous lots.

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-VEGF/VEGFA Antibody Picoband® catalog # PB9071. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-VEGF/VEGFA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9071)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

Isotype

Rabbit IgG

Immunogen

E.coli-derived human VEGF recombinant protein (Position: A27-R191). Human VEGF shares 78% amino acid (aa) sequence identity with both mouse and rat VEGF.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9071 is reactive to VEGFA in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

18, 27 kDa

Calculated molecular weight

Background of

VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. And this gene also can regulate haematopoietic stem cell survival by an internal autocrine loop mechanism. What’s more, it also stimulates neurogenesis in vitro and in vivo.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9071 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human

Positive Control

WB: human U87 whole cell, human K562 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
IHC: human ovarian serous adenocarcinoma tissue, human lung cancer tissue
FCM: K562 cell

Validation Images & Assay Conditions

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This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Apr 10, 2026