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VEGFA antibody (PB9071) performs well in Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 53 Publications

pb9071 vegfa primary antibodies fcm testing 1

Flow Cytometry analysis of K562 cells using anti-VEGF/VEGFA antibody (PB9071).
Overlay histogram showing K562 cells stained with PB9071 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-VEGF/VEGFA Antibody (PB9071, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-VEGF/VEGFA Antibody Picoband®

VEGFA antibody

53 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IHC, WB

Tested Applications

VEGFA antibody was positively detected by WB in:

human U87 whole cell, human K562 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

VEGFA antibody was positively detected by IHC in:

human ovarian serous adenocarcinoma tissue, human lung cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

VEGFA antibody was positively detected by FC in:

K562 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Mouse

Tested

Product Information

Product Name

Anti-VEGF/VEGFA Antibody Picoband®
View all VEGFA Antibodies

Form

Lyophilized

Uniprot

P15692

Gene ID

7422

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human VEGF recombinant protein (Position: A27-R191). Human VEGF shares 78% amino acid (aa) sequence identity with both mouse and rat VEGF.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

18, 27 kDa

Calculated Molecular Weight

27.0 kDa

Background of VEGFA

VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. And this gene also can regulate haematopoietic stem cell survival by an internal autocrine loop mechanism. What’s more, it also stimulates neurogenesis in vitro and in vivo.

Cite This Product

Anti-VEGF/VEGFA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9071)

Protocols

Western Blot protocol for VEGFA antibody

Review Protocol

Immunohistochemistry (IHC) protocol for VEGFA antibody

Review Protocol

Flow Cytometry (FC) protocol for VEGFA antibody

Review Protocol

Publications

Expired apheresis platelet-derived exosomes promote cutaneous wound healing

Published: 2026-08-03

Journal: Scientific Reports

Application: WB

Impact Factor: 4.90

Species: Human

PMID: 42686879

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Anti-VEGF/VEGFA Antibody Picoband®

VEGFA antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Expired apheresis platelet-derived exosomes promote cutaneous wound healing

Photocrosslinked organic-inorganic protein hydrogel with built-in nanoclusters for spatial biomineralization and enhanced osteogenesis

The Effects of Filgrastim and Hyaluronic Acid on the Endometrium in Experimentally Induced Asherman Syndrome Rat Models: A Prospective Laboratory Study

Inhibition of Ovarian Cancer Growth, Metastasis and Reverse the Tumor Microenvironment by Dual Drug-Loaded Polymer Micelle Targeting Tumor Microenvironment

The Effects of Methylprednisolone and Hyaluronic Acid on the Endometrium in Experimentally Induced Asherman Syndrome Rat Models: A Prospective Laboratory Study

Methotrexate-modified docetaxel liposome targeting with ginsenoside Rh2 as a membrane stabilizer for the treatment of ovarian cancer

Multifunctional Drug- and AuNRs-Loaded ROS-Responsive Selenium-Containing Polyurethane Nanofibers for Smart Wound Healing

Chondroitin sulfate-modified antiangiogenic peptide conjugate induces cell apoptosis via the mitochondria-mediated pathway to perform antitumor activity

VEGF overexpression in transplanted NSCs promote recovery of neurological function in rats with cerebral ischemia by modulating the Wnt signal transduction pathway

A microfluidic lung-on-a-chip based on biomimetic hydrogel membrane

Study of the mechanism of sonodynamic therapy in a rat glioma model

An oxidation responsive nano-radiosensitizer increases radiotherapy efficacy by remolding tumor vasculature

Construction of an AuHQ nano-sensitizer for enhanced radiotherapy efficacy through remolding tumor vasculature

Curcumin Derivative Cur20 Attenuated Cerebral Ischemic Injury by Antioxidant Effect and HIF-1α/VEGF/TFEB-Activated Angiogenesis

Hydrogel microfluidic-based liver-on-a-chip: Mimicking the mass transfer and structural features of liver

A Comparison of Ramipril and Bevacizumab to Mitigate Radiation-Induced Brain Necrosis: An Experimental Study

Anti-inflammatory effects of the root, stem and leaf extracts of Chloranthus serratus on adjuvant-induced arthritis in rats

Synergic effect of PD-1 blockade and endostar on the PI3K/AKT/mTOR-mediated autophagy and angiogenesis in Lewis lung carcinoma mouse model

The effect of Metapanax delavayi leaf extract on testosterone‐induced benign prostatic hyperplasia in rats

Ginsenoside protects against AKI via activation of HIF‑1α and VEGF‑A in the kidney‑brain axis

MiR-203a-3p inhibits retinal angiogenesis and alleviates proliferative diabetic retinopathy in oxygen-induced retinopathy (OIR) rat model via targeting VEGFA and HIF-1α

Effects of CO2 fractional laser on hair growth in C57BL/6 mice and potential underlying mechanisms

Targeted Delivery of Zoledronate to Tumor-Associated Macrophages for Cancer Immunotherapy

Effects of hypoxia on the biological behavior of MSCs seeded in demineralized bone scaffolds with different stiffness

Liquiritin from Glycyrrhiza uralensis Attenuating Rheumatoid Arthritis via Reducing Inflammation, Suppressing Angiogenesis, and Inhibiting MAPK Signaling Pathway

Recombinant human growth hormone (rhGH) treatment of MKN-45 xenograft mice improves nutrition status and strengthens immune function without promoting tumor growth

High thoracic sympathetic block improves coronary microcirculation disturbance in rats with chronic heart failure

Retracted: Curcumin derivative L6H4 inhibits proliferation and invasion of gastric cancer cell line BGC-823

LncRNA MIAT facilitated BM-MSCs differentiation into endothelial cells and restored erectile dysfunction via targeting miR-200a in a rat model of erectile dysfunction

Synthetic E-selectin prevents postoperative vascular restenosis by inhibiting nuclear factor κB in rats

An Experimental Study on Repeated Brief Ischemia in Promoting Sciatic Nerve Repair and Regeneration in Rats

The earlier, the better: the effects of different administration timepoints of sorafenib in suppressing the carcinogenesis of VEGF in rats

The Involvement of β-Catenin/COX-2/VEGF Axis in NMDA-Caused Retinopathy

Effect of tetramethylpyrazine combined with cisplatin on VEGF, KLF4 and ADAMTS1 in Lewis lung cancer mice

Effects of Chronic Exposure to Sodium Arsenite on Expressions of VEGF and VEGFR2 Proteins in the Epididymis of Rats

A macrophage-activating, injectable hydrogel to sequester endogenous growth factors for in situ angiogenesis

Synergistic effects of G-CSF and bone marrow stromal cells on nerve regeneration with acellular nerve xenografts

Ilexgenin A exerts anti-inflammation and anti-angiogenesis effects through inhibition of STAT3 and PI3K pathways and exhibits synergistic effects with Sorafenib on hepatoma growth

Enhanced antitumor activity and attenuated cardiotoxicity of Epirubicin combined with Paeonol against breast cancer

A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis

Buyanghuanwu decoction promotes angiogenesis after cerebral ischemia/reperfusion injury: mechanisms of brain tissue repair

Lentivirus-mediated PLCγ1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma

Expression of TP53, BCL-2, and VEGFA Genes in Esophagus Carcinoma and its Biological Significance

Overexpression of Astrocyte Elevated Gene-1 (AEG-1) in Cervical Cancer and its Correlation with Angiogenesis

Integration of donor mesenchymal stem cell-derived neuron-like cells into host neural network after rat spinal cord transection

Effect of vascular endothelial growth factor on retinal ganglion cells of rats with chronic intraocular hypertension

Accumulation of FLT3+ CD11c+ dendritic cells in psoriatic lesions and the anti-psoriatic effect of a selective FLT3 inhibitor

Correlations Between CT Perfusion Parameters and Vascular Endothelial Growth Factor Expression and Microvessel Density in Implanted VX2 Lung Tumors

Sciatic nerve repair by acellular nerve xenografts implanted with BMSCs in rats xenograft combined with BMSCs

Effects of panax notoginoside on the nephropathy in rats with type 1 diabetes mellitus

Decreased TIP30 Expression Promotes Tumor Metastasis in Lung Cancer

Extract of Ginkgo Biloba Promotes the Expression of VEGF Following Subarachnoid Hemorrhage in Rats

Induction stage-dependent expression of vascular endothelial growth factor and aquaporin-1 in diethylstilbestrol-treated rat pituitary

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