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Product Info Summary
| SKU: | A01931-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-KGF/FGF7 Antibody Picoband®
SKU/Catalog Number
A01931-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KGF/FGF7 Antibody Picoband® catalog # A01931-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KGF/FGF7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01931-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human KGF/FGF7 recombinant protein (Position: R54-K178).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01931-1 is reactive to FGF7 in Human
Observed Molecular Weight
22 kDa
Calculated molecular weight
22.5 kDa
Background of FGF7
Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01931-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human U20S whole cell
Validation Images & Assay Conditions
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Western blot analysis of KGF/FGF7 using anti-KGF/FGF7 antibody (A01931-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U20S whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KGF/FGF7 antigen affinity purified polyclonal antibody (Catalog # A01931-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KGF/FGF7 at approximately 22KD. The expected band size for KGF/FGF7 is at 22KD.
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The inductive ability of DPCs. (A) The intact DPC markers ALP, BMP2, and FGF7 were detected by Western blot. (B–F) HaCaT cells were cultured with conditioned medium from DPCs treated with virus expressing Tcf4, Twist1, and siTwist1. Differentiation markers KRT40, MSX2, KRT5, and KRT15 were detected by Western blot. (C–F) Quantitative analysis of (B) . The expression levels of target genes were standardized to that of GAPDH. * P < 0.05 when compared with the control group. ** P < 0.01 when compared with the control group. *** P < 0.001 when compared with the control group.
Index in PubMed under a CC BY license. PMID: 32974352
Specific Publications For Anti-KGF/FGF7 Antibody Picoband® (A01931-1)
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